Background: The rise of antibiotic resistance among methicillin resistant Staphylococcus aureus (MRSA), have caused concerns for the treatment of MRSA infections. Hence, search for an alternative therapy for these infections is inevitable. Folk Indian medicine refers to the use of leaf and stem bark powder of Tabernaemontana alternifolia (Roxb) in treatment of skin infections, but no scientific report establishes its antibacterial activity. Methods: Direct aqueous extracts and sequential aqueous extracts of the stem bark of T. alternifolia (using petroleum ether and ethyl acetate as other solvents) were prepared by soxhlet extraction. The antibiotic sensitivity profiles of the clinical isolates were determined against 18 antibiotics using disc diffusion method. The isolates were identified by 16S rRNA gene sequencing. The methicillin resistance among S. aureus (MRSA) was confirmed by PCR amplification of mecA gene. The disc diffusion method was used to determine the antibacterial activity of the extracts. The micro-dilution method was used to determine the minimum inhibitory concentration (MIC) of the extract against the test organism. To further evaluate the therapeutic potential of the extract, cell cytotoxicity was checked on Vero cells by MTT assay. Chemical profiling of the extract was done by HPTLC method. Results: The aqueous extracts of T. alternifolia stem bark exhibited antibacterial activity against Gram-positive microorganisms, particularly against clinical isolates of MRSA and vancomycin resistant S. aureus (VRSA). The minimum inhibitory concentration (MIC) of extract against the isolates ranged from 600–800 μg/ml. The extract did not exhibit cytotoxic activity against Vero cells even at the concentration of 4 mg/ml. The chemical profiling revealed presence of alkaloids, flavonoids, coumarins, saponins and steroids. Petroleum ether and ethyl acetate extracts did not exhibit antibacterial activity. Conclusion: Our results offer a scientific basis for the traditional use of T. alternifolia in the treatment of skin infections, showing that the plant extract has an enormous potential as a prospective alternative therapy against MRSA skin infections. The present study lays the basis for future studies, to validate the possible use of T. alternifolia as a candidate in the treatment of MRSA infections.

Sacred groves are relict forests dedicated to local deities that have been informally protected and managed by local communities over centuries. To gain greater insights into underlying sociocultural reasons for protection, and possibilities of strengthening such efforts, a study has been undertaken in 66 sacred groves in the Maharashtra State of Western Ghats biodiversity hot-spot in India, which shows different facets of human-nature relationships. Primary data on size, ownership, deity, religious aspects and awareness amongst the local people was collected through site visits, semi-structured interviews, and by participating in village meetings. It was observed that village councils owned 48% of the groves as against temple trusts (41%) and forest department plus individually owned groves (11%). Nearly 52% of the groves have an area less than one-half hectare. Forty-seven percent of the groves have male deities, 29% female deities and 26% were dedicated to spirits. Eighty percent of the local people were aware of cultural and religious aspects associated with the groves, whereas awareness about ecological and utilitarian values declined steeply to less than 20% of the population, necessitating the explanation of scientific conservation values of these critical ecosystems to the local people. The paper also discusses the significance of such studies in connection with the relevant UN Sustainable Development Goals and AICHI Biodiversity targets. In addition, it also discusses links with national biodiversity targets and how legal provisions under India’s Biological Diversity Act (2002) can be used to benefit both the ecosystem and the cultural values of the people, and how such informal institutions can be strengthened under formal protection mechanisms.

The Western Ghats of India support an array of tropical forests ranging from wet evergreen to scrub formations. Several endemic and threatened plant species are located in areas other than protected areas (PAs). There is an urgent need to understand species diversity in areas other than PAs, for effective management of tropical forests. In this context, reserve forests and informal PAs of Amboli from northern Western Ghats have been investigated. Woody species composition, diversity, and stand structure were assessed by laying quadrats and transects (n=46, area=2.575ha) in closed and open canopy forest patches covering habitat heterogeneity and environmental gradient of the area. A total of 2,224 individuals (of 87 species, 68 genera, and 35 families) was enumerated. Memecylon umbellatum, Syzygium cumini, and Diospyros nigrescens were found to be the most dominant species as per importance value index. Melastomataceae was the most dominant family as per family importance value, whereas Euphorbiaceae and Rutaceae were the most speciose. Fourteen IUCN Red List assessed species and 18 species endemic to the Western Ghats were encountered. Endemic species accounted for nearly 20% of the total number of individuals sampled. Demographic profile exhibited reverse ‘J’ pattern. Average basal area was 27.02m2 per hectare. Woody species diversity of Amboli forests was found comparable with other PAs from northern Western Ghats. Amboli and the adjoining area have been proposed as ecologically sensitive and in the wake of anthropogenic and developmental pressures they experience, it calls for urgent conservation attention.

Members of the insect order Odonata are known as good ecological indicators. Many are sensitive to habitat modifications and are easily monitored for use in environmental assessment studies. Rapid assessments rely on efficient sampling techniques. However, there is limited information available on sampling techniques for adult odonates, and protocols require evaluation. To do this, we standardized counting methods during sampling of odonates from August to November 2016 at the Mula River, Pune, India. We used four counting techniques; full-width belt transect (FWBT), full-circle point count (FCPC), half-width belt transect (HWBT), and half-circle point count (HCPC). For HWBT and HCPC areas facing the river were sampled, and for each technique we took multiple temporal replicates. We compared species detected per unit time, species detected per unit area, new species detected per unit time, and new species detected per unit area. Additionally, we compared species estimates. With HCPC we detected the maximum number of species and new species per unit area, whereas FWBT returned maximum coverage of recorded species. We recommend our proposed techniques be considered in the future across various habitats to decide the most suitable sampling strategy for the different habitats or situations.