The evolution of reproductive isolation (RI) results in the reduction of interspecific hybridization and the maintenance of species boundaries. Asymmetries in RI, where one species more frequently serves as the maternal or paternal parent in initial F₁ hybrid formation, are commonly observed in plants. Asymmetric introgression, the predominantly unidirectional transfer of genetic material through hybridization and backcrossing, has also been frequently documented in hybridizing plant taxa as well. This study investigates whether asymmetries in total RI measured between species can predict the direction of introgression in naturally hybridizing plant taxa. A meta-analysis was conducted on 19 plant species pairs with published data on both asymmetric total RI, and asymmetric introgression. Species pairs that met these criteria were identified through a comprehensive literature review. A two-tailed binomial test was performed to evaluate whether asymmetric RI was associated with asymmetries in introgression. No significant relationship was found between asymmetries in total RI and the direction of introgression (p = 0.3593). Asymmetric RI largely does not predict the direction of introgression. Rather, introgression patterns may be better understood by examining F₁ and later-generation hybrids in natural settings, focusing on their fitness, mating behaviors, and the ecological and demographic factors that shape hybrid zones.

Limiting harm to organisms caused by genetic sampling is an important consideration for rare species, and a number of non-destructive sampling techniques have been developed to address this issue in freshwater mussels. Two methods, visceral swabbing and tissue biopsies, have proven to be effective for DNA sampling, though it is unclear as to which method is preferable for genotyping-by-sequencing (GBS). Tissue biopsies may cause undue stress and damage to organisms, while visceral swabbing potentially reduces the chance of such harm. Our study compared the efficacy of these two DNA sampling methods for generating GBS data for the unionid freshwater mussel, the Texas pigtoe (Fusconaia askewi). Our results find both methods generate quality sequence data, though some considerations are in order. Tissue biopsies produced significantly higher DNA concentrations and larger numbers of reads when compared with swabs, though there was no significant association between starting DNA concentration and number of reads generated. Swabbing produced greater sequence depth (more reads per sequence), while tissue biopsies revealed greater coverage across the genome (at lower sequence depth). Patterns of genomic variation as characterized in principal component analyses were similar regardless of the sampling method, suggesting that the less invasive swabbing is a viable option for producing quality GBS data in these organisms.

Background/Objectives: The evolution of reproductive isolation (RI) results in the reduction of interspecific hybridization and the maintenance of species boundaries. Asymmetries in RI, where one species more frequently serves as the maternal or paternal parent in initial F1 hybrid for-mation, are commonly observed in plants. Asymmetric introgression, the predominantly unidi-rectional transfer of genetic material through hybridization and backcrossing, has also been fre-quently documented in hybridizing plant taxa as well. This study investigates whether asymme-tries in total RI measured between species can predict the direction of introgression in naturally hybridizing plant taxa. Methods: A meta-analysis was conducted on 19 plant species pairs with published data on both asymmetric total RI, and asymmetric introgression. Species pairs that met these criteria were identified through a comprehensive literature review. A two-tailed binomial test was performed to evaluate whether asymmetric RI was associated with asymmetries in in-trogression. Results: No significant relationship was found between asymmetries in total RI and the direction of introgression (p = 0.3593). Conclusions: Asymmetric RI largely does not predict the direction of introgression. Rather, introgression patterns may be better understood by exam-ining F1 and later-generation hybrids in natural settings, focusing on their fitness, mating behav-iors, and the ecological and demographic factors that shape hybrid zones.