A comprehensive meta-analysis was conducted to quantitatively assess the impact of dietary grape and grape by-products supplementation on growth performance, intestinal histomorphology, immunological response, and antioxidant status in broiler chickens. A systematic search guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 across PubMed, Scopus, and Web of Science up to October 19, 2025, yielded 349 records, of which 21 controlled feeding studies satisfied the inclusion criteria. Data were analyzed using a multilevel random-effects model in R (metafor package). Heterogeneity (I² and τ²) and publication bias were evaluated using Egger's regression and the trim-and-fill method. Pooled estimations indicated that grape supplementation did not significantly influence body weight gain (mean difference (MD) 12.7 g, 95% CI: -14.5 to 39.8, P = 0.360), feed intake (MD = 30.6 g, 95% CI: -51.8 to 113, P = 0.466), or feed conversion ratio (FCR) (MD = 0.01, 95% CI: -0.01 to 0.02, P = 0.565). Significant enhancements were observed in villus height (MD = 59.5 µm, 95% CI: 1.49-117, P = 0.044), reduction in crypt depth (MD = -13.3 µm, 95% CI: -18.7 to -7.95, P < 0.001), and villus height-to-crypt depth ratio (VH:CD) (MD = 1.31, 95% CI: 0.32-2.29, P = 0.009), indicating improved intestinal histomorphology and absorptive capacity. The humoral immune response showed a positive but non-significant effect (standardized mean difference (SMD) = 1.09, 95% CI: -0.69-2.87, P = 0.228). Enzymatic antioxidants (SMD = 0.25, P = 0.697), lipid peroxidation (SMD = -0.50, P = 0.297), and non-enzymatic antioxidant capacity (SMD = 2.45, P = 0.26) were also non-significant. Substantial heterogeneity across outcomes was mainly explained by grape product form, intestinal segment, immunological outcome type, and antioxidant marker type through meta-regression. Overall, grape by-products improved intestinal histomorphology but did not significantly enhance growth performance, immunity, or oxidative balance.

The flavor of chicken meat is formed by a series of complex chemical reactions, and the flavor precursors are affected by regulatory genes. In order to study the differences of muscle flavor precursors between Tengchong Snow chickens and AA broilers, integrated metabolomics and transcriptomics analyses were used to investigate muscle metabolite profiles and the key genes involved in the metabolism of muscle flavor compounds. The results showed that 42 significantly differentially metabolites were detected, and (5-L-Glutamyl)-L-glutamate, gamma-Glutamylalanine, S-Adenosylhomocysteine, Homo-L-arginine and GMP were important flavor metabolites. The key candidate genes with high correlation with flavor precursor metabolites were identified through correlation analysis as NME2 , AMPD1 , GADL1 , ASNS , CARNS1 , and CTH . In addition, the gene-metabolite interaction network for flavor formation in chicken breast muscle was constructed. This study could provide some basic data for the formation mechanism of local chicken excellent meat quality, and provide reference for the development and utilization of local chicken breeds and the selection and breeding of high-quality broilers.

Mycoplasma bovis (M. bovis) is a major pathogen responsible for bovine respiratory disease, mastitis, and arthritis, causing significant economic losses to the cattle industry worldwide. To elucidate the genetic and biological characteristics of M. bovis circulating in Yunnan Province, China, twenty PCR-positive bovine respiratory samples were collected from cattle farms in Kunming; three isolates—M.bo-YNXD-1, A1, and A8—were successfully cultured and identified through colony morphology, biochemical assays, and molecular characterization. Antimicrobial susceptibility testing showed that M.bo-YNXD-1 exhibited multidrug resistance to six antibiotics, including ciprofloxacin and lincomycin, while A1 and A8 were resistant to one or two agents, respectively. Multilocus sequence typing (MLST) analysis revealed that isolates M.bo-YNXD-1 and M.bo-YNXD-A8 belonged to sequence type ST52, whereas isolate M.bo-YNXD-A1 was assigned to ST90, indicating the coexistence of distinct genetic lineages in this region. Virulence gene screening showed that isolate M.bo-YNXD-A8 was positive for VspX and p81, whereas all three isolates were positive for p48 and Vpam. A SYBR Green I-based quantitative PCR (qPCR) assay targeting the oppD/F gene was established, exhibiting high specificity, a detection limit of 10 copies/μL, and intra-/inter-assay variation below 3%. Validation using clinical samples demonstrated superior sensitivity compared with conventional PCR. Taken together, these findings indicate the presence of distinct MLST genotypes and virulence-associated genetic heterogeneity among regional Mycoplasma bovis isolates, and introduce a rapid, sensitive, and reliable qPCR assay for early detection and epidemiological surveillance. This study provides critical insights for rational antimicrobial use and targeted control strategies against M. bovis infections.

Understanding the ecological adaptability of Pennisetum giganteum (JUJUNCAO) and its long-term effects on rhizosphere microecology is critical for vegetation restoration in fragile ecosystems. In this study, we investigated the soil physicochemical properties and microbial community structure and function in the rhizosphere of P. giganteum cultivated for 1 and 3 years (Y1, Y3), alongside pre-planting soil (Y0), in a dry-hot valley in Chuxiong, Yunnan, China. Following three years of cultivation, the soil total carbon (TC), organic carbon (SOC), total nitrogen (TN), nitrate nitrogen (NO₃⁻-N), total phosphorus (TP), and available phosphorus (AP) showed significant increases of 84.18%, 96.09%, 61.32%, 212.47%, 24.71%, and 22.19%, respectively, whereas the soil pH remained stable. Fungal communities showed significant declines in diversity and richness and a fundamental structural shift as early as one year after planting. In contrast, prokaryotic communities showed a relatively stable structure. Soil carbon and nitrogen variables were the factors most strongly associated with microbial community composition, and long-term cultivation concurrently enriched the arbuscular mycorrhizal fungi Septoglomus and genera containing potential plant pathogens such as Fusarium and Nectria. The relative abundance of Nectria was positively correlated with the soil carbon and nitrogen contents (p < 0.05), suggesting a potential ecological trade-off between beneficial symbiosis and disease risk. Predicted functional pathway composition indicated a shift in microbial metabolism from basal pathways of phospholipid and nucleotide synthesis toward carbon-nitrogen metabolism via PWY-3781 and the glyoxylate shunt, with fungal community turnover more pronounced. Consequently, P. giganteum demonstrates considerable potential for ecological restoration in dry-hot valleys; however, long-term cultivation deserves attention due to potential nitrate loss and the accumulation of taxa containing potential pathogens, with fungal communities serving as sensitive bioindicators of soil health.