The frequency shift of multipath sound rays induced by the motion of a sound source in an ocean waveguide environment is a crucial factor affecting the detection capabilities of both active and passive sonar systems, as well as the quality of underwater communication. Therefore, investigating the sound field characteristics of a moving sound source in the SOFAR channel is of significant importance. By comparing the spectra of continuous-wave (CW) signals with pulse widths of 1 s and 15 s received by a vertical array in SOFAR channel, it was observed that the sound field of the moving source exhibits a stable spectral splitting characteristic. Two frequency shift bright lines in the vertical direction were identified, corresponding to two sets of sound ray paths. One set of sound ray paths corresponds to the direct sound and the first surface-reflected sound, and the other set of sound ray paths corresponds to the first seabed-reflected sound and the first surface- and seabed-reflected sound. This study revealed that the spectral splitting of the moving sound source’s sound field displays a distribution trend in a depth direction similar to that of the multipath delay structure. A multipath sound ray frequency shift calculation model, based on ray theory, was developed to explain and predict the vertical distribution pattern of spectral splitting in the sound field of a moving sound source. By combining the model with measured data, it was found that the spectral splitting arises from the frequency shift differences corresponding to multipath sound ray paths. Additionally, the frequency shifts for the D&S and B&SB ray paths are generally proportional to the cosine values of the initial grazing angles of the sound waves at the emission source and the cosine values of the horizontal azimuthal angle between the source motion direction and the receiver.