The effects of NaCl and Na2SO4 on photosynthetic pigments, malondialdehyde (MDA), Rubisco activity and superoxide dismutase (SOD) activity were investigated in Kalidium foliatum(Pall.) Moq., which...

Salinity stress is a major abiotic factor that adversely affects plant growth and development. This study investigated the physiological and molecular responses of Brassica rapa L. ssp. Pekinensis to salinity stress by subjecting seedlings to varying concentrations of NaCl. Physiological analysis revealed significant wilting, chlorosis, and a marked reduction in chlorophyll and carotenoid contents in NaCl-treated seedlings, indicating impaired photosynthetic efficiency and oxidative stress mitigation. RNA-seq analysis identified extensive transcriptional reprogramming, with 6693 and 10,280 differentially expressed genes (DEGs) in Z150 and Z300 treatments, respectively, compared to the control group. DEGs were clustered into six expression trends, with sustained up-regulation in Clusters 2 and 6 and down-regulation in Cluster 3. Gene Ontology (GO) enrichment analysis highlighted the involvement of these DEGs in stress responses. Key DEGs encoding heat shock proteins, peroxidases, glutathione S-transferases, and transcription factors were significantly induced under salinity stress, suggesting their roles in stress adaptation. Furthermore, GO and KEGG enrichment analyses revealed significant down-regulation of genes associated with photosynthesis and carbon metabolism, indicating disruption of these critical pathways. Weighted Gene Co-expression Network Analysis (WGCNA) identified hub genes, such as histidine synthase and low-density receptor-like protein, potentially central to salinity stress responses. Additionally, carotenoid metabolism was significantly inhibited, with down-regulation of key genes in the carotenoid biosynthesis pathway. RT-qPCR validation confirmed the reliability of the RNA-seq data. Collectively, these findings provide comprehensive insights into the physiological and molecular mechanisms underlying response of B. rapa L. ssp. Pekinensis to salinity stress, highlighting potential targets for improving salinity tolerance in crops.

Suaeda salsa is a succulent halophyte belonging to Suaedoideae; sodium, chloride and sulfate ions or combination of the ions were useful to its growth. The seed germination is important event in...

Cynanchum thesioides (C. thesioides) is a sand-dwelling edible and medicinal plant whose fruit softens rapidly after harvest, limiting its storage life. In this study, we investigated the efficacy and underlying mechanisms of alternating magnetic field (AMF) treatment as a non-thermal and eco-friendly preservation method for C. thesioides fruit. Freshly harvested fruits were subjected to AMF at varying field intensities (1.07–1.54 mT) and exposure durations (5–25 min). We monitored the physiological indicators (respiration rate, membrane permeability, and firmness) during storage to determine the optimal conditions and performed transcriptome sequencing to identify differentially expressed genes, with qRT-PCR validation of two key cell wall-degrading genes (β-glucosidase (BG) and polygalacturonase (PG)). The results showed that AMF treatment at 1.28 mT for 15 min best maintained the postharvest quality, significantly reducing respiration and membrane leakage while delaying firmness loss. Transcriptomic analysis identified 2480 differentially expressed genes enriched in hormone signaling and cell wall metabolism pathways, and qRT-PCR confirmed that AMF downregulated BG and PG expression, suggesting suppressed cell wall degradation and delayed softening. In conclusion, AMF treatment effectively prolonged the shelf life of C. thesioides by modulating the expression of cell wall-related genes. These findings provide novel insight into magnetic field-induced fruit preservation and support AMF as a green non-thermal postharvest technology.