Chitosan, a naturally occurring biopolymer derived from chitin, has emerged as a highly promising instrument for the production and application of metal nanoparticles. The present review delves into the several functions of chitosan in the development and operation of metal nanoparticles, emphasizing its aptitudes as a green reducing agent, shape-directing agent, size-controlling agent, and stabilizer. Chitosan’s special qualities make it easier to manufacture metal nanoparticles and nanocomposites with desired characteristics. Furthermore, there is a lot of promise for chitosan-based nanocomposites in a number of fields, such as metal removal, water purification, and photoacoustic, photothermal, antibacterial, and photodynamic therapies. This thorough analysis highlights the potential application of chitosan in the advancement of nanotechnology and the development of medicinal and environmental solutions.

Medicinal benefits of honey bee have been recognized in the medical community since ancient times as a remedy for many diseases and infections. This study aimed to investigate the in vitro susceptibility of 11 multidrug-resistant bacterial strains, isolated from urinary tract infections of pregnant women, to six honey samples collected from different localities in the east of Algeria. The evaluation of the antibacterial activity was performed by the well method followed by the broth dilution method using two-fold dilutions of each honey sample ranging from 2.5 to 80% (w/v). The results obtained in this study revealed that all tested honeys exhibited potent antibacterial activity against the tested strains. The diameters of inhibition ranged from 19.67 to 53.33 mm, with minimum inhibitory concentrations (MICs) ranging from 2.5 to 40% (w/v) and minimum bactericidal concentration (MBCs) varied between 2.5 and 80% (w/v). Gram-positive bacteria were found to be more susceptible than Gram-negative bacteria with diameters ranging from 43.33 to 53.33 mm; MIC and MBC values ranged from 2.5 to 5% (w/v). The P. aeruginosa strain was found to be less susceptible than other strains with inhibitory diameters ranging from 19.67 to 27.33 mm; MICs ranged from 20 to 40% and MBCs ranged from 20 to 80% (w/v). This contribution has provided a broad overview of the antibacterial activity of Algerian honey and shown that honey bee has great potential for therapeutic use as an alternative therapy for urinary tract infection treatment which is safe and efficient during pregnancy.

Typha latifolia leaves act as sensitive barometers for trace heavy metal pollution, as revealed by their pronounced anatomical responses in a constructed wetland. Monthly water samples and Typha latifolia leaf tissue were collected over three consecutive months in 2018 from the Burgas Lake wetlands (Taoura), northeast Algeria. While physical and chemical parameters improved after treatment, atomic absorption spectrometry (Perkin Elmer A Analyst 800 AAS) detected persistent trace levels of cadmium, chromium, and lead in both the treated water and leaf tissue, highlighting the need for continued phytoremediation efforts. Microscopic examination of leaf tissue exposed to these metals revealed distinct anatomical adaptations, including shrunken vascular bundles, altered cell shapes, and stomatal closure. These findings underscore Typha latifolia’s effectiveness in accumulating heavy metals and its potential as a highly sensitive biomonitor for persistent pollution in lake ecosystems.

The Oued Zénati, a vital waterway in Algeria, faces severe pollution from urban discharges, hospital wastewater, and agricultural activities, threatening both the ecosystem and public health. This pollution is characterized by high nutrient levels, suspended solids, and fecal contamination indicators, jeopardizing biodiversity and human well-being. To explore natural restoration solutions, this study assessed the purification potential of reeds (Phragmites australis) found in the Oued Zénati riverbed. Water quality was analyzed at three sites: a non-polluted control site (S1), a wastewater discharge area (S2), and a reed-dense area (S3). Results revealed a significant deterioration in water quality at site S2, with high concentrations of nutrients, suspended solids (SS), and fecal contamination indicators. However, a notable improvement in water quality was observed at site S3, downstream of the reed-dense area, with reductions in fecal coliforms (68.5%), fecal streptococci (92.3%), and phosphates (40.3%), and increased levels of dissolved oxygen (DO). These findings suggest that phytoremediation using P. australis could offer a cost-effective, sustainable, and eco-friendly solution for restoring the Oued Zénati. This study recommends establishing phragmifiltration stations, developing artificial wetlands, and enhancing sanitation systems, including hospital wastewater treatment. Public awareness campaigns promoting water and environmental protection are crucial for long-term success. This phytoremediation approach offers economic, ecological, and aesthetic advantages over conventional wastewater treatment techniques.

This study investigated the microbiological and parasitic quality of sewage sludge treated in drying beds in Algeria, aiming to contribute to a better understanding of the factors influencing sludge safety for potential agricultural applications in the Algerian context. The research focused on various sludge types (liquid, semi-solid, and solid) and their behavior across different seasons. Standard microbiological methods were employed to quantify total coliforms, fecal streptococci, E. coli, and Clostridium. Controls were implemented to ensure accuracy, with positive controls validating detection methods using known quantities of microorganisms and parasites, while negative controls confirmed the absence of contamination in the testing environment. Parasitic contamination was assessed through microscopic examination for protozoa and helminths. Results revealed substantial variation in microbial concentrations across sludge types and seasons. Liquid sludge, particularly during summer, exhibited the highest levels of total coliforms (up to 7.021 log10) and E. coli (up to 6.049 log10), while solid sludge showed lower counts. Seasonal trends indicated increased microbial levels during warmer months. Parasitic contamination was prevalent in 81% of samples, with protozoan cysts (e.g., Giardia intestinalis and Endolimax nanus) and helminth eggs detected. Despite reducing microbial loads, drying processes alone were insufficient, leaving significant contamination. Enhanced protocols are needed, such as longer drying periods, chemical disinfectants, or advanced technologies like anaerobic digestion or composting. This highlights the need for locally adapted treatment strategies. Furthermore, this research provides specific recommendations for improving sewage sludge management practices in Algeria, taking into account the unique environmental and agricultural context of the country.

The present study used the adult earthworm Aporrectodea trapezoides as a bioindicator species to look into the possible dangers of ammonium sulfate (AS) fertilizer. Two complementary toxicity tests were conducted to determine the LC50values, growth rate inhibition, morphological alterations, and histopathological texture of worms. The lethality test included four increasing concentrations of AS fertilizer (ranging from 2500 to 7500 mg/kg of dry soil weight (d.w.)), while sub-lethal concentrations were based on 10%, 30%, 40%, and 50% of the 14-day median lethal concentration (LC50), with a control group included for both tests. The LC₍₅₀₎ values for AS fertilizer were significantly higher at 7 days (4831.13 mg/kg d.w.) than at 14 days (2698.67 mg/kg d.w.) of exposure. Notably, earthworms exhibited significant growth rate inhibition under exposure to various concentrations and time durations (14/28 exposure days). Morphological alterations such as clitellar swelling, bloody lesions, whole body coiling and constriction, body strangulation, and fragmentation were accentuated steadily, with higher concentrations. Histopathological manifestations included severe injuries to the circular and longitudinal muscular layers, vacuolation, muscle layer atrophy, degradation of the chloragogenous tissue in the intestine, collapsed digestive epithelium of the pharynx with weak reserve inclusion, and fibrosis of blood vessels. These effects were primarily influenced by increasing concentrations of fertilizer and time exposure. The study highlights the strong relationship between concentration and exposure time responses and underscores the potential of A. trapezoides earthworms as valuable biological control agents against acidic ammonium sulfate fertilizer. Importantly, this research contributes to the use of such biomarkers in evaluating soil toxicity and the biological control of environmental risk assessment associated with chemical fertilizers.