The use of medicinal plants represents an alternative method for bacterial control due to their chemical compositions. This study’s objective was to determine the inhibitory capacity of Larrea tridentata extracts against microbial strains of clinical interest. Four extracts were prepared, their phytochemical profiles were determined, and their antioxidant capacities were quantified. Additionally, the minimum concentrations of hemolysis were determined using human blood erythrocytes. For the extracts’ growth inhibitory capacity, six bacterial and two fungal strains were evaluated using the disk diffusion test. Commercial medications specific for each strain were used as controls. The ethanolic extracts registered the greatest diversity of metabolites related to antibacterial activity. The inhibitory activities of the ethanolic extract and the Cedax^® control were similar for Enterococcus faecalis. A principal component analysis was performed with X² and ANOVA tests to identify the relationships and the effects of the extracts on bacterial inhibition, obtaining p > 0.05 with a confidence level of 95%. This research highlights the potential of L. tridentata extracts as an alternative treatment and to mitigate the growing problem of resistance to traditional antibiotics.