The leaf area index (LAI) is of great significance for crop growth monitoring. Recently, unmanned aerial systems (UASs) have experienced rapid development and can provide critical data support for crop LAI monitoring. This study investigates the effects of combining spectral and texture features extracted from UAS multispectral imagery on maize LAI estimation. Multispectral images and in situ maize LAI were collected from test sites in Tongshan, Xuzhou, Jiangsu Province, China. The spectral and texture features of UAS multispectral remote sensing images are extracted using the vegetation indices (VIs) and the gray-level co-occurrence matrix (GLCM), respectively. Normalized texture indices (NDTIs), ratio texture indices (RTIs), and difference texture indices (DTIs) are calculated using two GLCM-based textures to express the influence of two different texture features on LAI monitoring at the same time. The remote sensing features are prescreened through correlation analysis. Different data dimensionality reduction or feature selection methods, including stepwise selection (ST), principal component analysis (PCA), and ST combined with PCA (ST_PCA), are coupled with support vector regression (SVR), random forest (RF), and multiple linear regression (MLR) to build the maize LAI estimation models. The results reveal that ST_PCA coupled with SVR has better performance, in terms of the VIs + DTIs (R² = 0.876, RMSE = 0.239) and VIs + NDTIs (R² = 0.877, RMSE = 0.236). This study introduces the potential of different texture indices for maize LAI monitoring and demonstrates the promising solution of using ST_PCA to realize the combining of spectral and texture features for improving the estimation accuracy of maize LAI.