Carnosine is a dipeptide synthesized in the body from β-alanine and L-histidine. It is found in high concentrations in the brain, muscle, and gastrointestinal tissues of humans and is present in all vertebrates. Carnosine has a number of beneficial antioxidant properties. For example, carnosine scavenges reactive oxygen species (ROS) as well as alpha-beta unsaturated aldehydes created by peroxidation of fatty acid cell membranes during oxidative stress. Carnosine can oppose glycation, and it can chelate divalent metal ions. Carnosine alleviates diabetic nephropathy by protecting podocyte and mesangial cells, and can slow down aging. Its component, the amino acid beta-alanine, is particularly interesting as a dietary supplement for athletes because it increases muscle carnosine, and improves effectiveness of exercise and stimulation and contraction in muscles. Carnosine is widely used among athletes in the form of supplements, but rarely in the population of cardiovascular or diabetic patients. Much less is known, if any, about its potential use in enriched food. In the present review, we aimed to provide recent knowledge on carnosine properties and distribution, its metabolism (synthesis and degradation), and analytical methods for carnosine determination, since one of the difficulties is the measurement of carnosine concentration in human samples. Furthermore, the potential mechanisms of carnosine’s biological effects in musculature, metabolism and on immunomodulation are discussed. Finally, this review provides a section on carnosine supplementation in the form of functional food and potential health benefits and up to the present, neglected clinical use of carnosine.

The effects of consumption of n-3 polyunsaturated fatty acids (n-3 PUFAs) enriched hen eggs on endothelium-dependent and endothelium-independent vasodilation in microcirculation, and on endothelial activation and inflammation were determined in young healthy individuals. Control group (N = 21) ate three regular hen eggs/daily (249 mg n-3 PUFAs/day), and n-3 PUFAs group (N = 19) ate three n-3 PUFAs enriched hen eggs/daily (1053 g n-3 PUFAs/day) for 3 weeks. Skin microvascular blood flow in response to iontophoresis of acetylcholine (AChID; endothelium-dependent) and sodium nitroprusside (SNPID; endothelium-independent) was assessed by laser Doppler flowmetry. Blood pressure (BP), body composition, body fluid status, serum lipid and free fatty acids profile, and inflammatory and endothelial activation markers were measured before and after respective dietary protocol. Results: Serum n-3 PUFAs concentration significantly increased, AChID significantly improved, and SNPID remained unchanged in n-3 PUFAs group, while none was changed in Control group. Interferon-γ (pro-inflammatory) significantly decreased and interleukin-10 (anti-inflammatory) significantly increased in n-3 PUFAs. BP, fat free mass, and total body water significantly decreased, while fat mass, interleukin-17A (pro-inflammatory), interleukin-10 and vascular endothelial growth factor A significantly increased in the Control group. Other measured parameters remained unchanged in both groups. Favorable anti-inflammatory properties of n-3 PUFAs consumption potentially contribute to the improvement of microvascular endothelium-dependent vasodilation in healthy individuals.

The research investigates the possibilities of enriching eggs with n-3 polyunsaturated fatty acids, selenium, vitamin E, and lutein. The research was carried out on 100 TETRA SL laying hens divided into two groups (C and E). Hens in treatment C (control group) were fed a standard feeding mixture that contained 5% soybean oil, 0.32 mg/kg organic selenium, 25.20 mg/kg vitamin E, and 20.5 mg/kg lutein (Marigold flower extract). Hens in treatment E (experimental group) were fed a mixture in which soybean oil was replaced by 1.5% fish oil + 1.5% rapeseed oil + 2.0% linseed oil. The content of other nutricines amounted to 0.47 mg/kg organic selenium, 125.2 mg/kg vitamin E, and 120.5 mg/kg lutein. Portions of total n-3 polyunsaturated fatty acids in enriched eggs were significantly increased when compared to conventional eggs (480.65:204.58 mg/100 g; p < 0.001). The content of selenium, vitamin E, and lutein was significantly higher (p < 0.001) in enriched eggs than in conventional eggs. The content of selenium in egg albumen was increased by 1.81 times, and in the yolk, it was increased by 1.18 times. At the same time, the content of vitamin E was 2.74 times higher, and lutein was 8.94 times higher in enriched eggs than in conventional eggs.

This research elaborates the process of enriching table eggs with n-3 polyunsaturated fatty acids (n-3 PUFA) and presents the effect of such enriched eggs on human health. The experiment was performed on 480 TETRA SL laying hens divided into three groups. Feeding mixtures contained 5% of oils (K = soybean oil, P1 = 3.5% linseed oil + 1.5% fish oil, P2 = 3% linseed oil + 2% fish oil). Referring to the content of α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), eggs of P1 and P2 groups were significantly richer in n-3 PUFA than eggs of the control group (p = 0.001). Atherogenic (AI), thrombogenic (TI), and hypo/hypercholesterolemic (HHI) indexes of egg yolks were more favourable in enriched eggs than in conventional eggs. Fatty acid profiles in the blood of examinees that consumed conventional and enriched eggs (treatments K and P1, respectively) differed significantly in total saturated fatty acids (ΣSFA) (p = 0.041) and in the content of ALA (p = 0.010). The consumption of n-3 PUFA-enriched eggs lowered the Σn-6 PUFA/Σn-3 PUFA ratio in the examinees’ blood serum (27%) and had a favourable effect on some blood biochemical indicators. This research confirmed the assumption that the use of a combination of fish and linseed oil in mixtures for laying hens in an amount of up to 5% will increase the content of omega-3 in table eggs, but it was not confirmed that the consumption of these eggs in a short period of time (21 days) has a positive effect on human health.

The aim of our study was to determine the effects of selenium supplementation to broiler feed on the content of selenium, total fatty acids in lipids and on the oxidative stability of broiler thigh muscle tissue. The experiment involved 40 broilers fattened for 42 days. During the first three weeks, all broilers consumed starter diet containing 22% crude protein. After three weeks, broilers were divided into two groups and fed finisher diets containing 18% crude protein and supplemented with 3% sunflower oil and 3% linseed oil. Group 1 was not administered artificial selenium; Group 2 was supplemented with organic selenium at the amount of 0.5 mg Se/kg of feed. Significantly higher ( P < 0.05) content of selenium was observed in the thigh muscle tissue of Group 2 compared to Group 1 (1.071 and 0.511 mg/kg of dry matter, respectively). The increase of selenium content in feed to 0.5 mg/kg resulted in an increase of total n3 polyunsaturated fatty acids (from 5.27% to 6.25%), and in reduction of total monounsaturated fatty acids (from 39.60% to 36.74%) in the lipids of thigh muscle tissue. The increase in total n-3 polyunsaturated fatty acids induced significant ( P < 0.05) lowering of the n6/n3 polyunsaturated fatty acids ratio (4.62 and 5.01 for the first and second group, respectively). The increase of selenium content in broiler feed led to a decrease of thiobarbituric acid reactive substances ( P > 0.05) and increase of linolenic acid and total n-3 polyunsaturated fatty acids ( P < 0.05) in thigh muscle tissue of broilers. Since selenium and n-3 polyunsaturated fatty acids are nutricines, our results show that the produced broiler meat may be considered as functional food.

This paper investigates the influence of vegetable oils (sunflower oil (SO), rapeseed oil (RO), and linseed oil (LO)) supplemented into broiler diets on the fatty acids profile and quality of lipids in breasts and thighs, expressed through qualitative, nutritional, and metabolic indices. Broilers of the Ross 308 hybrid were divided into three groups and fattened over 6 weeks. During the last three weeks of fattening, broilers were fed a finisher diet that differed in types of supplemented oil. Diets were balanced at the level of 19.80% crude protein and 13.5 MJ ME/kg of feed. Feed and water were offered to broilers ad libitum. Fatty acid profiles were determined in samples of broiler breasts and thighs, based on which lipids health indices were calculated. Quantitative indices (polyunsaturated fatty acids-∑PUFA/saturated fatty acids-∑SFA, ∑PUFA n-6/∑PUFA n-3, linoleic acid-LA/α-linolenic acid-ALA, and eicosapentaenoic acid-EPA+ docosahexaenoic acid-DHA) were influenced by the type of oil (p < 0.001), whereas the type of meat was only important for the index of ∑PUFA/∑SFA (p < 0.001). Nutritional indices (nutritional value index-NVI, atherogenicity index-AI, thrombogenicity index-TI, hypo/hypercholesterolemic index-hHI, health-promoting index-HPI) depended on both factors (p < 0.001). The metabolic indices (elongase index-EI, thioesterase index-THI, ∆9-desaturase, ∆5 + ∆6-desaturase, kinetic activity index-KHI) were significantly affected by the type of meat (p < 0.001), as well as by the feeding treatments (p < 0.05), except for the EI index. This research confirmed that oils supplemented into broiler feed influence the fatty acid profiles in broiler meat. It has also been confirmed that the fatty acid profile affects the lipid quality in meat, which may be beneficial for consumers’ health.