Background: The Signal Transducer and Activator of Transcription 1 (STAT1) gene is an essential component of the JAK-STAT signaling pathway. This pathway plays a pivotal role in the regulation of different cellular processes, including immune responses, cell growth, and apoptosis. Mutations in the STAT1 gene contribute to a variety of immune system dysfunctions. Objectives: We aim to identify disease-susceptible single-nucleotide polymorphisms (SNPs) in STAT1 gene and predict structural changes associated with the mutations that disrupt normal protein–protein interactions using different computational algorithms. Methods: Several in silico tools, such as SIFT, Polyphen v2, PROVEAN, SNAP2, PhD-SNP, SNPs&GO, Pmut, and PANTHER, were used to determine the deleterious nsSNPs of the STAT1. Further, we evaluated the potentially deleterious SNPs for their effect on protein stability using I-Mutant, MUpro, and DDMUT. Additionally, we predicted the functional and structural effects of the nsSNPs using MutPred. We used Alpha-Missense to predict missense variant pathogenicity. Moreover, we predicted the 3D structure of STAT1 using an artificial intelligence system, alphafold, and the visualization of the 3D structures of the wild-type amino acids and the mutant residues was performed using ChimeraX 1.9 software. Furthermore, we analyzed the structural and conformational variations that have resulted from SNPs using Project Hope, while changes in the biological interactions between wild type, mutant amino acids, and neighborhood residues was studied using DDMUT. Conservational analysis and surface accessibility prediction of STAT1 was performed using ConSurf. We predicted the protein–protein interaction using STRING database. Results: In the current study, we identified six deleterious nsSNPs (R602W, I648T, V642D, L600P, I578N, and W504C) and their effect on protein structure, function, and stability. Conclusions: These findings highlight the potential of approaches to pinpoint pathogenic SNPs, providing a time- and cost-effective alternative to experimental approaches. To the best of our knowledge, this is the first comprehensive study in which we analyze STAT1 gene variants using both bioinformatics and artificial-intelligence-based model tools.

Burn prevention programs can effectively reduce morbidity and mortality rates. In this article, we present the findings of our investigation of the knowledge, attitudes, and practices of the Saudi Arabian population regarding electrical burns. Our study was a cross-sectional online survey that used a five-part questionnaire to assess the participant’s demographic information, knowledge of electrical burns, attitudes toward electrical injuries, and practices related to electrical burns and their prevention. Overall, 2314 individuals responded to the survey (males: 41.2%; females: 58.8%). A total of 839 participants (36%) had a personal or family history of electrical burns. Approximately ≥90% of the responses to questions on electrical burn-related knowledge were correct; relatively less responses to questions on the extent of tissue damage from electrical burns and arcs were correct (74% and 29%, respectively). Only 54% of the respondents knew that applying first aid to the burn-affected areas at home could lead to a better outcome; 27% and 19% did not know the correct answer and thought that this would not lead to a better outcome, respectively. The most common source of information was school or college (38.9%), followed by social media (20.8%) and internet websites (16.3%). Enhancing community awareness and practices related to electrical burns is a cost-effective and straightforward strategy to prevent the morbidity and mortality associated with electrical injuries.

Introduction: While the literature is replete of clinical studies reporting on the Robin sequence (RS), population-based analyses are scarce with significant variability within the literature in terms of reported incidence, demographic parameters, and outcomes. The authors have conducted a 20-year population-based analysis to guide clinical practice. Methods: A birth cohort was created from the available datasets in the Healthcare Cost and Utilization Project—Kids’ Inpatient Database (HCUP-KID; 2000–2019). Robin sequence patients were identified and further stratified by syndromic status. Incidence, demographic parameters, and outcomes including mortality and tracheostomy rates were computed. A subset analysis comparing the isolated and syndromic cohorts was conducted. Data was analyzed through a χ² or t test. Results: The incidence of RS was 5.15:10,000 (95% CI: 4.99–5.31) from a birth cohort of 7.5 million. Overall, 63.3% of the cohort was isolated RS and 36.7% had syndromic RS. Robin sequence patients had a significantly higher rate of cardiac (25.9%) and neurological (8.6%) anomalies compared with the general birth cohort and were most commonly managed in urban teaching hospitals (P<0.0001). The pooled mortality and tracheostomy rates were 6.6% and 3.6%, respectively. Syndromic status was associated with a longer length of hospital stay (27.8 versus 13.6 d), tracheostomy rate (6.2% versus 2.1%), and mortality (14.1% versus 2.2%) compared with isolated RS (P<0.0001). Conclusions: The true incidence of RS is likely higher than previously reported estimates. Isolated RS patients have a low associated mortality and tracheostomy rate and are typically managed in urban teaching hospitals. Syndromic status confers a higher mortality rate, tracheostomy rate, and length of stay compared with nonsyndromic counterparts.

A 40-year-old female presented with right mandibular swelling. A panoramic radiograph showed a unilocular radiolucency from the mandibular angle to tooth #46. Biopsy confirmed a schwannoma. Surgical resection was performed via a submandibular approach with a reconstruction plate. Teeth 46 and 47 were extracted. Surgery was complication-free, and histopathology confirmed the tumor’s benign nature. The patient was discharged on the second postoperative day. At the 1-year follow-up, she had no paresthesia, normal mouth opening, and full mandibular motion. The reconstruction plate was intact. This case adds to the limited literature on intraosseous schwannomas, emphasizing early detection, thorough radiological assessment, and meticulous surgical planning.

Over-resuscitation is a formidable complication of burn resuscitation and increased morbidity and mortality. Currently, the Advanced Trauma Life Support recommends using a modified Brooke's formula to minimise its incidence; however, supporting evidence is very limited. We aimed to compare the resuscitative and clinical outcomes between the Parkland and modified Brooke's formulas in patients with burn trauma. A retrospective cohort study was conducted through a chart review of patients admitted to the burn unit. The patients were divided into two groups: Group 1 (2017-2019) was resuscitated using Parkland's formula and Group 2 (2020-2022) with modified Brooke's formula. The main outcome measures were mortality rate, length of stay, complication rate exceeding the Ivy index (250 mL/kg) in the first 24 h, and overall fluid administered in the first 24 h. We included 125 patients, of whom fifty-five were resuscitated using Parkland's formula and seventy using the modified Brooke's formula. Patients in group 1 had higher resuscitation volume (5.04 vs. 3.37 mL/kg/total body surface area, P < 0.0001) and were more likely to exceed the Ivy index (250 mL/kg) (32.73% vs. 12.86%, P = 0.0074) in the first 24 h compared to those in group 2. There were no significant differences in clinical outcomes between the two groups. The modified Brooke's formula resulted in lower resuscitative volumes in the first 24 h than Parkland's formula, with no differences in complication rates. Our findings are consistent with currently recommended guidelines.

Background: Age-related changes in the brow region, such as sagging, asymmetry, and deepening of forehead lines, pose significant cosmetic and functional challenges. Various surgical approaches have been developed over the decades to address these issues. This systematic review compared traditional open techniques versus endoscopic and minimally invasive brow lift techniques in terms of aesthetic outcomes and complication rates. Methods: A comprehensive search was conducted in PubMed, MEDLINE, the Cochrane Library, Embase, and ClinicalTrials.gov. Studies published during the past 20 years that met our predefined eligibility criteria were included. The quality of randomized controlled trials was assessed using the Cochrane Risk of Bias 2 tool, and nonrandomized studies were evaluated with risk of bias in non-randomized studies of interventions (ROBINS-I). Data on aesthetic outcomes and complications were extracted and compared across studies. Results: Fifteen studies were included in this review. The evidence suggests that although various open brow lift techniques provide satisfactory elevation, they are generally associated with higher complication rates and less favorable cosmetic results. In contrast, endoscopic and minimally invasive techniques, including the use of Endotine devices and gliding brow lifts, demonstrate comparable or superior aesthetic outcomes with lower complication rates and reduced recovery times. However, heterogeneity in outcome measures and study designs limits the strength of these conclusions. Conclusions: Endoscopic and minimally invasive brow lift procedures seem to offer cosmetically favorable results with lower complication rates compared with traditional open techniques. Nevertheless, further high-quality, prospective studies are needed to definitively establish the long-term efficacy and safety of these approaches.