Objective: Reduced crude protein (CP) diets offer potential benefits such as optimized feed efficiency, reduced expenses, and lower environmental impact. The objective of this study was to evaluate black soldier fly larvae (BSFL) meal on a low-protein diet for duck performance, blood biochemical, intestinal morphology, gastrointestinal development, and litter.Methods: The experiment was conducted for 42 days. A total of 210-day-old male hybrid ducklings (5 replicate pens, 7 ducks per pen) were randomly assigned to 6 dietary treatments (3×2 factorial arrangements) in randomized design. The factors were CP level (18%, 16%, 14%) and protein source feed soybean meals (SBM), black soldier fly larvae meals (BSFLM).Results: Reduced dietary CP levels significantly decreased growth performance, feed intake, the percentage of nitrogen, pH (p<0.05), and tended to suppress ammonia in litter (p = 0.088); increased lipid concentration; and enhanced relative weight of gastrointestinal tracts (p<0.05). In addition, dietary BSFL as a source of protein feed significantly increased lipid concentration and impacted lowering villus height and crypt depth on jejunum (p<0.05).Conclusion: In conclusion, the use of BSFLM in a low-protein diet was found to have a detrimental effect on growth performance. However, the reduction of 2% CP levels in SBM did not have a significant impact on growth performance but decreased nitrogen and ammonia concentrations.

Protected sodium butyrate (PSB) is considered an alternative to antibiotics in broiler diets. The present study investigated the effect of PSB supplementation on growth performance, carcass traits, relative weight of internal organs and intestinal morphology in broilers. A total of 1200...

| Galangal essential oil (EO) has a promising impact as a rumen modifier because it contains 24% of cineole and known to have antimicrobial activity on Gram positive and Gram negative bacteria. Improving the quality of milk composition can be achieved by modification of microbial abundance in the rumen. The study was conducted to measure milk production and composition of Holstein Friesian cow ration, which was supplemented with galangal (EO). The experiment was designed a completely randomized design, 60% of mott elephant grass and 40% of concentrate (dry matter basis) were supplemented by galangal EO as much as 0, 1.25 and 2.50 g/head/day with four replications. The data on milk production were collected every day of milking. Milk composition (lactose, protein, and fat) were analyzed each week for 30 days, and milk fatty acids were analyzed at the end of the research. The results showed no significant difference in milk production, milk composition among rations. Furthermore, milk urea nitrogen (MUN) and blood urea nitrogen (BUN) concentration was also absorbed and galangal EO did not effect on both treatments. Galangal EO at dose of 1.25 g/head/day decreased significantly on blood of volatile fatty acids. The concentration of unsaturated fatty acids (ɣ-linolenic acids) ratio 1.25 was higher than 0 and 250 g/head/day. In conclusion, Galangal EO supplementation increased the polyunsaturated fatty acids ratio at level 1.25 g/head/day of galangal EO, but did not affect milk production, lactose, protein, and fat content.

Black soldier fly larvae (BSFL) oil has gained attention as a potential substitute for vegetable oil in feed formulations designed to improve productivity and product quality. This study aimed to investigate the impact of supplementation BSFL oil calcium salt (BSFLO-SCa) on the egg quality and gene expression involved in fat metabolism in laying hens. A total of sixty 40-wk-old ISA Brown laying hens were divided into three distinct groups: a control group (T0) that received a basic diet, and two treatment groups that were supplemented with 1% (T1) and 2% (T2) BSFLO-SCa. Each group consisted of 5 replicates, with 4 laying hens per replicate. The study indicated that the inclusion of 2% of BSFLO-SCa significantly increased egg albumen weight, height, ratio, eggshell weight, and thickness ( ). The inclusion of 1 and 2% of BSFLO-SCa increased the yolk lightness (L*) and yellowness (b*), but redness (a*) was decreased ( ). Moreover, BSFLO-SCa supplementation with 1 and 2% reduced ( ) egg fat and cholesterol. Analysis of fatty acid profiles showed significantly increased medium-chain fatty acid (MCFA) and polyunsaturated fatty acid (PUFA) ( ), while monosaturated fatty acid (MUFA) reduced ( ) with 2% BSFLO-SCa. Gene expression related to lipid metabolism, such as fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC), as well as cholesterol synthesis gene expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) were downregulated ( ). In conclusion, the inclusion of 2% BSFLO-SCa in the diet improved quality and reduced fat and cholesterol content in eggs from laying hens. This improvement was accompanied by downregulation of key genes involved in lipid metabolism.

Lactic Acid Bacteria (LAB) are bacteria that produce lactic acid and are very useful bacteria, one of which can act as a probiotic because it can produce bacteriocins and produce peptide compounds with high biological activity. Colostrum is the first fluid that comes out of the udder of the mother cattle after giving birth which is very good for newborns because it contains antibodies. This research aimed to isolate, selection and identify the types of LAB found in dairy cow colostrum, especially LAB which have superior proteolytic activity. LAB obtained is expected to have a positive effect on livestock productivity. This research was conducted In-vitro in a laboratory. The research procedure included the isolation of LAB from the colostrum of dairy cows, then the selection procedure by looking at the best protease enzyme activity qualitatively and quantitatively, and testing for resistance to low pH. Lactic acid isolation, selection, and identification were analyzed using descriptive analysis and quantitative analysis using one-way ANOVA for a completely randomized design. The results obtained 6 superior proteolytic LAB isolates based on the protease enzyme activity coded LAB pro 4, LAB pro 3, LAB pro 8, LAB pro 9, LAB pro 21, and LAB pro 20.

Feed efficiency is essential for dairy cows because it can increase production and quality of milk, and reduce methane emissions in the environment. One of the strategies on increasing feed efficiency is using rumen modifier. Essential oils have antimicrobial activities and have been used in rumen modifier in dairy cows. The aim of this paper is to review several studies on the utilization of essential oils as feed additive to enhance production and quality of milk of dairy cow. Based on some research reports, using essential oils which contained thymol, eugenol, cinnamaldehyde and carvacrol in dairy cattle feed decreased the population of protozoa, methanogens, proteolytic, and biohydrogenase bacteria. The impact of engineering of the rumen microbial population has led to optimal rumen fermentation resulted in the efficiency of carbohydrate, protein, and fat utilizations. It can be concluded that essential oils are potential as rumen modifier by inhibit methanogenesis, increase propionate, energy efficiency and ruminal by pass protein. The inconsistent results on milk production and milk fat requires further research to study deeper the effect of dosage and type of essential oils on dairy cows performance.