Purpose: The soil microbial community is an important bioactive component of terrestrial ecosystems. Its structural and functional diversity directly affects carbon and nitrogen processes. This study aimed to investigate the variations in the diversity and composition of soil bacterial communities in a wetland with different nitrogen deposition conditions. Methods: A long-term simulated nitrogen deposition experiment was conducted in the Ecological Locating Research Station of the Institute of Nature and Ecology of Heilongjiang Academy of Sciences. Three different treatments were evaluated, including low nitrogen (LK; 40 kg N·hm^-2·a^-1), high nitrogen (HN; 80 kg N·hm^-2·a^-1), and control (CK; 0 kg N·hm^-2·a^-1). Bacterial 16S rDNA was then sequenced and analyzed using the next-generation sequencing technology. Result: Higher levels of N deposition resulted in an α-diversity increase followed by a decrease, with significant reductions in the HN treatment. Simulated nitrogen deposition resulted in changes in the structure and abundance of bacterial communities in wetland soils. The dominant phyla in all three plots were Proteobacteria and Acidobacteria. Compared with CK, the relative abundance of Chloroflexi increased significantly under the HN treatment (P < 0.05), whereas the relative abundance of Firmicutes and Bacteroidetes decreased significantly (P < 0.05). Nitrogen input changed the composition and relative abundance of the bacterial community, which was possibly due to N-induced soil acidification. Conclusion: This study thus provides a theoretical basis for predicting the effects of atmospheric nitrogen deposition on soil microorganisms, as well as changes in the wetland ecosystem in Sanjiang Plain.

Research Highlights: Understanding of the spatial variation of root exudation on a regional scale can help understand the response of plant physiological activities to environmental changes. Background and Objectives: Although root exudation has become an important topic in belowground ecology, its relationship with root traits and environmental factors is poorly understood. Our objective was to explore how root traits and environmental factors influence root exudation. Materials and Methods: We used a multi-factorial design consisting of three tree species spanning across sites located at three latitudes to assess root exudation dynamics, which was measured using a syringe-basis incubation system. Results: The strongest and clearest effect observed in our study was a decrease in root exudation rates of Korean pine (Pinus koraiensis Sieb. et Zucc.) and larch (Larix gmelinii (Rupr.) Kuze.) at sites located in higher latitudes. Root exudation rates were positively related to mean annual temperature, mean annual precipitation, and negatively related to soil total organic carbon. Conclusions: Root exudation in coniferous species decreased at sites located in higher latitudes. Despite differences in root exudation rate among sites located at different latitudes and species with suitable variation in root morphological traits and environmental factors, we could not identify consistent influencing factors on root exudation rates.

To clarify the characteristics of soil aggregate breakdown and transportation by rainfall, the cultivated land (0–10 cm) of the black soil region of Northeast China was taken as the research object, with rainfall intensities of 78 and 127 mm·h⁻¹ (RI₇₈, RI₁₂₇), to analyze the differences in the mass transported, fraction size distribution, mean weight diameter (MWD) and enrichment rate of aggregates before and after runoff generation. Before runoff generation, the total mass of transported aggregates, the total mass percentage of the aggregate fraction size < 1 mm and the MWD of the transported aggregates were not significantly different at rainfall intensities of 78 and 127 mm·h⁻¹. After runoff generation, the mass of transported aggregates was greater than that before runoff generation, and the mass of transported aggregates under RI₇₈ was significantly higher than that under RI₁₂₇, by 15.6%. The mass percentage of the aggregate fraction size < 0.053 mm after runoff generation was significantly reduced by 8.4% and 19.4% compared to that before runoff generation. After runoff generation, compared with before runoff generation, the MWD of the transported aggregates was significantly reduced by 50.9% and 50.3% under RI₇₈ and RI₁₂₇, respectively. Compared with before runoff generation, the mass percentage of small macroaggregates increased gradually with the increase in the transport distance. The aggregate fraction size > 0.25 mm was lost, and the fraction size < 0.25 mm was enriched, before and after runoff generation. A comparative study on the characteristics of black soil aggregate breakdown and transportation before and after runoff generation can provide a theoretical basis for the mechanism of soil erosion and the transportation of cultivated soil in the black soil region of Northeast China.

The soil microbial community structure is critical to the cycling of carbon and nitrogen in forest soils. As afforestation practices increasingly promote different functional traits of tree species, it has become critical to understand how they influence soil microbial community structures, which directly influence soil biogeochemical processes. We used fungi ITS and bacteria 16S rDNA to investigate soil microbial community structures in three monoculture plantations consisting of a non-native evergreen conifer (Pinus sibirica), a native deciduous conifer (Larix gmelinii), and a native deciduous angiosperm (Betula platyphylla) and compared them with two 1:1 mixed-species plantations (P. sibirica and L. gmelinii, P. sibirica and B. platyphylla). The fungal community structure of the conifer–angiosperm mixed plantation was similar to that of the non-native evergreen conifer, and the bacterial community structure was similar to that of the angiosperm monoculture plantation. Fungal communities were strongly related to tree species, but bacterial communities were strongly related to soil nitrogen. The co-occurrence networks were more robust in the mixed plantations, and the microbial structures associated with soil carbon and nitrogen were significantly increased. Our results provide a comparative study of the soil microbial ecology in response to afforestation of species with different functional traits and enhance the understanding of factors controlling the soil microbial community structure.

Roots anchor plants firmly to the soil, enabling them to effectively resist soil erosion and shear failure. Vegetation restoration has been acknowledged as one of the most useful measures for controlling soil loss; however, which root system characteristics were most beneficial for plant anchoring in the soil remains unclear. In the black soil region of northeastern China, which frequently experiences serious soil erosion, pullout tests were carried out on six species of soil and water conservation woody plants with different growth habits (deciduous shrubs, deciduous trees and evergreen trees), and the root geometry and topology of each species were determined. The results showed that the maximum uprooting force and activation displacement (the displacement at the maximum peak in the relationship curve between pulling force and displacement) of shrubs were significantly greater than those of trees, while deciduous trees were significantly greater than evergreen trees. Therefore, the ability of the whole root system to anchor the soil was the largest for shrubs, followed by deciduous trees, and the smallest for evergreen trees. The uprooting force and activation displacement were mainly affected by the root topological index, total root length and the number of inclined roots. The total root length had the greatest influence on the maximum uprooting force, and the root topology had the greatest influence on the activation displacement, both of which can be used as important predictors of plant root anchorage strength. In addition, the plants with the R-type root structure may have a greater ability to anchor the soi, and can be prioritized for vegetation restoration with black soils. These findings provide references and implications for identifying the effective plant strategies for eroded soil restoration in the black soil region of northeastern China and other areas with similar soil types and bioclimates.