In this study, a low-cost, self-balancing crop spectral reflectance sensor (CSRS) was designed for real-time, nondestructive monitoring of the spectral reflectance and vegetation index of crops such as tomato and rapeseed. The sensor had a field of view of 30°, and a narrow-band filter was used for light splitting. The filter’s full width at half-maximum was 10 nm, and the spectral bands were 710 nm and 870 nm. The sensor was powered by a battery and used WiFi for communication. Its software was based on the Contiki operating system. To make the sensor work in different light intensity conditions, the photoelectric conversion automatic gain circuit had a total of 255 combinations of amplification. The gimbal of the sensor was mainly composed of an inner ring and an outer ring. Under the gravity of the sensor, the central axis of the sensor remained vertical, such that the up-facing and down-facing photosensitive units stayed in the horizontal position. The mechanical components of the sensor were designed symmetrically to facilitate equal mass distribution and to meet the needs of automatic balancing. Based on the optical signal transmission process of the sensor and the dark-current characteristics of the photodetector, a calibration method was theoretically deduced, which improved the accuracy and stability of the sensor under different ambient light intensities. The calibration method is also applicable for the calibration of other crop growth information sensors. Next, the standard reflectance gray scale was taken as the measurement variable to test the accuracy of the sensor, and the results showed that the root mean square error of the reflectance measured by the sensor at 710 nm and 870 nm was 1.10% and 1.27%, respectively; the mean absolute error was 0.95% and 0.89%, respectively; the relative error was below 4% and 3%, respectively; and the coefficient of variation was between 1.0% and 2.5%. The reflectance data measured by the sensor under different ambient light intensities suggested that the absolute error of the sensor was within ±0.5%, and the coefficients of variation at the two spectral bands were 1.04% and 0.39%, respectively. With tomato and rapeseed as the monitoring targets, the proposed CSRS and a commercial spectroradiometer were used to measure at the same time. The results showed that the reflectance measured by the two devices was very close, and there was a linear relationship between the normalized difference vegetation index of the CSRS and that of the commercial spectroradiometer. The coefficient of determination (R²) for tomato and rapeseed were 0.9540 and 0.9110, respectively.

The Hongze Lake is the fourth largest freshwater lake in China and an important lake for the East Route of the South-to-North Water Diversion Project. The water quality of the lake affects social development and the lives of residents. To assess the impacts of environmental changes and human activities on the distribution of the total suspended matter (TSM) in the Hongze Lake, we developed an algorithm that utilizes the near-infrared (NIR) band to estimate TSM based on in situ measurements. The algorithm was applied to Landsat images to derive TSM distribution maps from 1984 to 2019, revealing significant inter-annual, seasonal, and spatial variability. The relationship between TSM, precipitation, and wind speed was analyzed, and we found that: (1) The estimation model of TSM concentration in the Hongze Lake constructed for TM and OLI has a high accuracy, and it can be used to jointly monitor TSM concentration in the Hongze Lake for long-term series; (2) From 1984 to 2019, the concentration of TSM in the Hongze Lake showed a trend of first rising and then falling, with the maximum value in 2010 at 100.18 mg/L mainly caused by sand mining activities. Precipitation and wind speed weakly influence the inter-annual variation of TSM concentration; (3) The concentration of TSM in the Hongze Lake in summer is easily affected by flooding in the Huai River, and the concentration of TSM in other seasons is significantly negatively correlated with precipitation; (4) TSM is highest in the Huaihe Bay, followed by the Lihe Bay and Chengzi Bay. The main reason for this is that the input of the Huaihe Bay flows directly into this lake area and is also the main navigation channel. The results of this study are of great significance for the protection and management of the water environment of the Hongze Lake.

Phosphorus is the most important nutrient associated with lake eutrophication and changes in cyanobacterial blooms, and particulate phosphorus (PP) is the main form of phosphorus found in highly turbid inland waters. Therefore, it is urgent to monitor PP concentrations in inland water bodies. In this study, we take Hongze Lake as the research area and establish a semianalytical model to estimate PP concentrations based on the total particle absorption coefficient (a_p); the mean absolute percentage error (MAPE) and root-mean-square error (RMSE) values, which indicate the model accuracy, were 14.90% and 0.009 mg/L, respectively. In addition, the construction process and parameter selection criteria of the remote sensing-based PP concentration estimation model were derived using remote sensing data obtained at different spectral resolutions. Sentinel 3 Ocean and Land Color Instrument (OLCI) and Landsat 9 Operational Land Imager version 2 (OLI-2) data were selected as representatives to verify the accuracy of the model; compared to these two datasets, the MAPE values of the models were 16.32% and 26.84%, respectively, while the RMSE values were 0.010 mg/L and 0.014 mg/L, respectively. Finally, the models were applied to Sentinel 3 OLCI and Landsat 9 OLI-2 images obtained on 16 January 2022. The results show that the spatiotemporal distributions of PP concentrations in Hongze Lake estimated from these two images were relatively consistent, but the OLI data reflected overestimations and underestimations in some areas. These research results provide a new methodology for estimating PP concentrations through remote sensing methods and help to further improve the accuracy of remotely sensed PP concentration estimations in inland water bodies.

Macrolide pollution has attracted a great deal of attention because of its ecotoxic effects on microalgae, but the role of phycospheric bacteria under antibiotic stress remains unclear. This study explored the toxic effects of erythromycin (ERY) on the growth and nitrogen metabolism of Auxenochlorella pyrenoidosa; then, it analyzed and predicted the effects of the composition and ecological function of phycospheric bacteria on microalgae under ERY stress. We found that 0.1, 1.0, and 10 mg/L ERY inhibited the growth and chlorophyll of microalgae, but the microalgae gradually showed enhanced growth abilities over the course of 21 days. As the exposure time progressed, the nitrate reductase activities of the microalgae gradually increased, but remained significantly lower than that of the control group at 21 d. NO₃⁻ concentrations in all treatment groups decreased gradually and were consistent with microalgae growth. NO₂⁻ concentrations in the three treatment groups were lower than those in the control group during ERY exposure over 21 d. ERY changed the community composition and diversity of phycospheric bacteria. The relative abundance of bacteria, such as unclassified-f-Rhizobiaceae, Mesorhizobium, Sphingopyxis, Aquimonas, and Blastomonas, varied to different degrees. Metabolic functions, such ABC transporters, the microbial metabolism in diverse environments, and the biosynthesis of amino acids, were significantly upregulated in the treatments of higher concentrations (1.0 and 10 mg/L). Higher concentrations of ERY significantly inhibited nitrate denitrification, nitrous oxide denitrification, nitrite denitrification, and nitrite and nitrate respiration. The findings of this study suggest that phycospheric bacteria alleviate antibiotic stress and restore the growth of microalgae by regulating nitrogen metabolism in the exposure system.

Hyperspectral data are important for water color remote sensing. The inevitable noise will devalue its application. In this study, we developed a 1-D denoising method for water hyperspectral data, based on sparse representing. The denoising performance was compared with three commonly used methods in simulated and real datasets. The results indicate that: (1) sparse representing can successfully decompose the hyperspectral water-surface reflectance signal from random noises; (2) the proposed method exhibited better performance compared with the other three methods in different input signal-to-noise ratio (SNR) levels; (3) the proposed method effectively erased abnormal spectral vibrations of field-measured and remote-sensing hyperspectral data; (4) whilst the method is built in 1-D, it can still control the salt-and-pepper noise of PRISMA hyperspectral image. In conclusion, the proposed denoising method can improve the hyperspectral data of an optically complex water body and offer a better data source for the remote monitoring of water color.