Understanding the genetic characteristics of Aeromonas jandaei in Brazilian aquaculture is crucial for developing effective control strategies against this fish pathogen. The present study conducted a genomic analysis of Brazilian A. jandaei strains with the objective of investigating their virulence potential and resistance profiles. Four Brazilian isolates were subjected to sequencing, and comparative genomic analyses were conducted in conjunction with 48 publicly available A. jandaei genomes. The methods employed included quality assessment, de novo assembly, annotation, and analyses of antimicrobial resistance and virulence factors. The results demonstrated the presence of fluoroquinolone resistance genes within the core genome. Notably, these antibiotics are not authorized for use in aquaculture in Brazil, suggesting that their resistance determinants may originate from other selective pressures or horizontal gene transfer unrelated to aquaculture practices. The analysis identified significant virulence mechanisms, including T2SS, T3SS, and notably T6SS (vgrG3 gene), which was more prevalent in Brazilian isolates. Additionally, genes associated with motility, adhesion, and heavy metal resistance were identified. These findings highlight the enhanced adaptability of Brazilian A. jandaei strains and raise concerns about antimicrobial resistance in aquaculture, emphasizing the need for improved regulatory oversight and control strategies.

Anais do Simpósio Estadual de Produtos Naturais (978-65-5941-203-7) - Mapeamento Biotecnológico Do Alecrim-Pimenta

Anais do Simpósio Estadual de Produtos Naturais (978-65-5941-203-7) - Potencial Biotecnológico Do Gênero Desmodesmus

Klebsiella pneumoniae is an important nosocomial pathogen, considered a critical threat to public health, but its genomic data are scarce in Brazil. Therefore, the aim of this study was to identify the mechanisms of virulence and antimicrobial resistance and to evaluate the molecular epidemiology of K. pneumoniae isolates from an outbreak in a university hospital in Brazil. The DNA of the 25 isolates was sequenced, the functional annotation of the genome was performed in Prokka, for virulence and resistance analyses PanViTa was used, and for Multilocus Sequence Typing analyses pyMLST was used. The isolates presented several virulence genes such as those of the fim operon, ecp operon, and T6SS (identified in all isolates), which are related to adhesion, biofilm formation, and toxin delivery. Other predicted virulence genes involve nutritional/metabolic factors, effector delivery systems, and immune modulation, while resistance genes mainly involve efflux (AcrAB-TolC, KpnGH-TolC, OqxAB, and KpnEF), antibiotic target alteration, reduced permeability, and antibiotic inactivation. In addition, the resistance genes identified in K. pneumoniae isolates are related to a total of 28 antimicrobials from different classes. The sequence types (ST) 11, 273, 395, 5209 and 636 were identified, this being the first report of the ST636 in Brazil. Genomic analysis confirmed the presence of complex virulence and antimicrobial resistance mechanisms, highlighting the threat these pathogens pose and raising concerns about the introduction of ST636 into Brazil, emphasizing the complexity of local strains and the need for continuous surveillance.

Anais do Simpósio Estadual de Produtos Naturais (978-65-5941-203-7) - Mapeamento De Tecnologias Envolvendo A Macaxeira

Internacional de Saúde Única (Interface Mundial) (978-85-5722-461-2) - Diagnóstico E Tratamento Da Doença De Chagas: Uma Sondagem Patentária Dos Últimos 10 Anos