Virtual reality (VR), among other technologies, is establishing the direction of development in the rehabilitation field. Diagnostic tools in cervical spine movement assessments have been described in many studies. The aim of this study was to present the potential of using VR for diagnosing movement quality disorders in the cervical spine. Additional objectives of the research include determining whether Virtual Reality conditions are reliable and objective for measuring the range of motion of the cervical spine when using the proprietary VR Head Tracking application, as well as assessing the level of satisfaction and tolerance of this type of diagnostic method among the subjects. We reached the following conclusions: the measurements of the cervical spine range of motion conducted using the proprietary VR Head Tracking application are reliable and objective, the level of satisfaction with the diagnostic method, as well as the tolerance of the VR examination, is consistently favorable in adults, the results obtained from the VR application allow for a deeper analysis of the data, beyond just the angles of the range of motion. The use of diagnostic methods in a Virtual Reality environment for assessing the biomechanics of the musculoskeletal system in the cervical spine is valid and can be efficiently and non-invasively carried out.