Handling and stability are crucial performance characteristics of motorcycles. However, handling and stability are always in opposition, which leads to difficulty in evaluating the comprehensive performance of a motorcycle. This article proposes an evaluation indicator to assess the comprehensive handling and stability performance of different motorcycles based on slalom experiments. We first confirmed differences in ‘roll factors’ among different motorcycles through slalom simulation. Subsequently, we proposed an evaluation index that took rider factors into account through experimental research. Finally, an objective evaluation based on a fuzzy analytic hierarchy process method was conducted. The result indicates a high degree of consistency between the normalized objective evaluation and subjective evaluation of different motorcycles.