Artificial intelligence (AI) plays a crucial role in modern healthcare by enhancing disease modeling and outbreak prediction. In this study, we develop an epidemiological model for the Marburg virus, integrating vaccination and treatment strategies while considering vaccine efficacy and treatment failure. The model exhibits mathematical symmetry in its equilibrium analysis, ensuring a balanced assessment of disease dynamics across human and bat reservoir populations. We compute the Marburg-free and endemic equilibrium points, derive the secondary infection threshold, and conduct sensitivity analysis using the PRCC method to identify key disease transmission parameters that are important for disease control. To validate the theory, we optimized a deep neural network (DNN) via grid search and employed it for dynamic analysis, which also validates the cutting-edge application of AI in healthcare. We also compare AI-based predictions with traditional numerical solutions for reproduction number for humans $R_{0h}>1$ and $R_{0h}<1$ for validation and efficacy of the AI approach. The results demonstrate the model’s stability, efficacy, and predictive power, emphasizing the synergy between AI and mathematical epidemiology. This study provides valuable insights for public health interventions and effective disease control strategies by leveraging AI-driven simulations, highlighting AI’s potential to revolutionize and enhance early detection and tailor treatment strategies.