[Purpose] This study examined the effects of moderate-to-heavy exercise training combined with weighted vest and chicken protein supplementation in older adult females.[Methods] Fifty-two female participants, 60–79 yearsold, were randomly divided into three groups: control (non-exercise) (CON), exercise with weighted vest (WV), and exercise with weighted vest and protein supplementation (3 g of protein daily; WVP).A ll participants performed brisk walking and strengthening exercises for 50 min/day, three times/week, for 8 weeks. Muscle mass, bone mineral content, T-score, and physical performance were measured.[Results] The WVP group demonstrated a substantial increase in thoracic spine bone mineral content (8.4 ± 7.7 g, p = 0.001), and total body lean mass (0.2 ± 1.0 kg) compared with that in the other two groups. The CON and WV groups showed a decrease in thoracic spine bone mineral content (CON= -1.8 ± 1.8, WV= -3.9 ± 0.1 g) and total body lean mass (CON= -0.7 ± 0.9, WV= -0.8 ± 0.9 kg) compared with those in the WVP group. The WVP and WV groups had increased T-score (WVP= 0.01 ± 0.16, WV= 0.02 ± 0.18) when compared with that of the CON group (-0.19 ± 0.12). Both the WV and WVP groups displayed improved physical performance compared with that of the CON group.[Conclusion] Combined exercise with either a weighted vest or protein supplementation proved to be effective in protecting against trunk bone and muscle mass loss, and improving physical fitness in older female adults.

Excessive high-fat diet (HFD) intake can precipitate metabolic dysfunction-associated fatty liver disease (MAFLD). Tangeretin is a citrus flavonoid possessing many biological properties. We examined the impact of tangeretin on MAFLD and its underlying mechanism. Rats were given HFD plus 15% fructose solution for four months to produce metabolic syndrome. Metabolic syndrome rats were administered 100 mg/kg of metformin or 25 mg/kg of tangeretin for the last four weeks. HFD-induced increased body weight, liver weight, adipose tissue weight, fasting blood glucose, serum insulin, total triglyceride, total cholesterol, and free fatty acids and reduced adiponectin and high-density lipoprotein cholesterol levels in metabolic syndrome, which were alleviated by tangeretin (p < 0.05). Tangeretin stabilized alanine transaminase activity, liver catalase, and inflammatory and oxidative stress markers in HFD rats compared to untreated HFD rats (p < 0.05). Tangeretin reduced hepatic steatosis induced by HFD. Downregulation of hepatic insulin receptor substrate-1 (IRS-1) and protein kinase B (Akt) protein expression in metabolic syndrome rats was recovered by tangeretin (p < 0.05). Metformin, an antihyperglycemic drug, produced comparable effects to tangeretin. In conclusion, tangeretin attenuates metabolic disorders and fatty liver induced by HFD in rats. The underlying mechanisms involve reducing oxidative stress, and inflammation and enhancing insulin sensitivity.

Low-load resistance training combined with vascular occlusion or hypoxia can increase muscle cross-sectional area (CSA), but the effect of such training on hormonal response and cardiovascular response is less clear. Thirty female netball athletes took part in a 5-week training of knee muscles in which low-load resistance exercise (20% 1-RM) was combined with either an occlusion pressure (KT, n = 10), hypoxic air (HT, n = 10), or no additional stimulus (CT, n = 10). Growth hormones (GHs), cardiovascular parameters, and CSA were measured before and after the training program. Compared to CT, both HT and KT showed a substantial increase in GH release after the first training bout (pre). After 5 weeks of training (post), the release of GH was substantially reduced in all groups. Compared to CT, HT showed a substantial decrease in SP (11.7 ± 11.3%, mean ± 90% CL) over the training period. The reduction in systolic blood pressure (SP) after hypoxic training resulted in a substantial decrease in the rate-pressure product (RPP) by 15.6 ± 9.6%, compared to CT. CSA from HT and KT is likely related to the heightened release of GH found after training. The hypoxic training protocol has a greater cardiovascular benefit than similar resistance training with blood flow restriction.

Background: The growing elderly population faces health problems like osteoporosis, but novel exercises like weighted vests (WV) and whole-body vibration (WBV) may help prevent bone loss. Methods: Thirty-one women aged 60–79 years with osteopenia or osteoporosis (T-score −2.15 ± 0.9) were randomly assigned to three groups: a control group performed exercise only; a whole-body vibration group performed the exercise on a 40 Hz, 2 mm vibration platform; and a weighted vest group performed the exercise while wearing a weighted vest. T-score, bone mineral density (BMD), muscle mass, and physical performance were assessed before and after 8 weeks. Results: The WV showed a greater T-score increase than the CT and WBV groups (WV: 0.08 ± 0.03; CT: −0.18 ± 0.04; WBV: −0.11 ± 0.16; p = 0.01, 95%CI). Leg BMD increased in the WV group (1.75 ± 0.13 to 1.79 ± 0.16 g/cm²; p = 0.02). Leg lean mass also increased in the WV (1.28 ± 0.91 kg) compared to WBV (0.17 ± 0.14 kg) and CT (0.06 ± 0.79 kg, p = 0.01, 95%CI). The WV group showed greater physical performance improvements (5TSTS and 6-MWT). The WBV group showed improved total lean mass compared to the CT group (WBV: 0.32 ± 0.17; CT = −1.20 ± 1.86, p = 0.006, 95%CI). Conclusions: WV exercise improved bone density, leg lean mass, and physical performance in older women. WBV exercise increased total lean mass and skeletal muscle index while reducing fat mass. WV exercise provides an additive effect beyond exercise alone or WBV.

Introduction: Resistance training enhances muscle strength and size; however, high-load exercise (>85% 1RM) may increase the risk of injury. Low-load resistance training with hypoxia or blood flow restriction (BFR) offers safer alternatives, although comparative data are limited. Objective: To examine the effects of hypoxia and BFR on resting growth hormone (GH) levels after five weeks of low-load resistance training. Methods: Thirty male athletes (19–24 years) were assigned to three groups: RT (50% 1RM), RT + HPX (50% 1RM under hypoxia, FiO₂ = 0.137), and RT + BFR (50% 1RM with BFR). Participants performed knee extension and flexion (3 sets × 15 reps, 1-min rest) three times weekly for five weeks. Muscle thickness, strength, resting GH, and blood lactate levels were measured before and after training. Results: After training, RT + HPX and RT + BFR showed significant increases in rectus femoris and biceps femoris thickness. Strength improved in all groups, with knee extension strength higher in the RT+HPX (30.9 ± 16.3%, p = 0.047) than RT (16.1 ± 7.3%). The resting GH levels did not differ significantly between the groups (p > 0.05). Post-exercise lactate increased significantly only in the RT+BFR (68.7 ± 57.2%, p = 0.018). Conclusion: Low-load training with hypoxia or BFR enhances muscle hypertrophy and strength, and hypoxia produces greater strength gain. Resting GH levels appear to be unrelated to these adaptations, suggesting that further studies are needed to clarify the underlying mechanisms.