Background/Objective: Periodontal disease is a prevalent inflammatory condition affecting the supporting structures of teeth, with radiographic bone loss (RBL) being a critical diagnostic marker. The accurate and consistent evaluation of RBL is essential for the staging and grading of periodontitis, as outlined by the 2017 AAP/EFP Classification. Advanced tools such as deep learning (DL) technology, including Denti.AI, an FDA-cleared software utilizing convolutional neural networks (CNNs), offer the potential for enhancing diagnostic accuracy. This study evaluated the diagnostic accuracy of Denti.AI for detecting RBL in intraoral radiographs. Methods: A dataset of 39 intraoral radiographs (22 periapical and 17 bitewing), covering 316 tooth surfaces (123 periapical and 193 bitewing), was selected from a de-identified pool of 500 radiographs provided by Denti.AI. RBL was assessed using the 2017 AAP/EFP Classification. A consensus panel of three board-certified dental specialists served as the reference standard. Performance metrics, including sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), accuracy, and mean absolute error (MAE), were calculated. Results: For periapical radiographs, Denti.AI achieved a sensitivity of 76%, specificity of 86%, PPV of 83%, NPV of 80%, and accuracy of 81%, with an MAE of 0.046%. For bitewing radiographs, sensitivity was 65%, specificity was 90%, PPV was 88%, NPV was 70%, and accuracy was 76%, with an MAE of 0.499 mm. Conclusions: Denti.AI demonstrated clinically acceptable performance in detecting RBL and shows potential as an adjunctive diagnostic tool, supporting clinical decision-making. While performance was robust for periapical radiographs, further optimization may enhance its accuracy for bitewing radiographs.