This experiment investigated the role of the gut microbiota in the growth and development of Antheraea pernyi, isolated gut bacteria related to lignin degradation in oak trees, and preliminarily verified their lignin degradation ability, providing a new tool for the field of biodegradation. The changes in the structure of the gut microbial community of the oak silkworm Antheraea pernyi at different developmental stages were analyzed via high-throughput sequencing of the 16SrDNA genes of the gut microbiota of fifth-instar larvae. A series of biological analyses were conducted, including amplicon sequence variant (ASV), microbial community diversity, microbial community composition, species difference, and evolutionary analyses. Ralstonia, Achromobacter, Pseudomonas, Cupriavidus, Bacteroidetes, Enterococcus, and Bacillus were the dominant bacterial groups in the gut microbiota of the larvae, with Ralstonia comprising the highest proportion, thereby providing a reference basis for a deeper understanding of the functions of the gut microbiota in insect growth and development. In this study, we activated and identified the MYZ028 strain isolated from the fifth-instar larvae of Antheraea pernyi through molecular biology, and we determined that the strain belonged to Bacillus subtilis. In terms of performance, the strain exhibited a high lignin-degrading ability and could effectively degrade lignin in a short time and simple environment. Due to the complex and difficult nature of degrading the lignin structure, research on the degradation of lignin by composite strains can be explored in the field of biodegradation.

Antheraea pernyi is a large silk-producing insect belonging to the family Saturniidae of the order Lepidoptera