High portability and environmental safety (“green”) are two of the most important objectives pursued by microfluidic methods. However, there remain many challenges for the design of portable and visual microfluidic methods (e.g., chip electrophoresis) due to use of a cumbersome pump, power supply and detector. Herein, a facile double inner standard plot (DISP) model of electrophoresis titration (ET) was proposed for portable and visual assay of proteins in test milk samples without use of a pump, power supply or detector based on a moving reaction boundary (MRB) chip. The DISP-ET model predicted that: (i) by setting the upper limit (UL) and lower limit (LL) of double inner standard milk protein contents, points U and L were, respectively, achieved in the relationship D = −aC + b (D: MRB motion distance; C: protein content); and (ii) the two points divided both the C-axis and D-axis into “poor”, “eligible” and “superior” rulers scaled for quantitative assay of test samples. To demonstrate the model of DISP-ET, an original portable device (120 mm × 78 mm × 30 mm, 341 g) was designed, which had a chip (25 mm × 25 mm × 4 mm) of three channels (15 mm × 200 μm × 80 μm), platinum electrodes, a lithium cell and touch screen. A series of experiments were undertaken based on the developed portable device. The relevant experiments demonstrated systemically the validity of the DISP-ET model, theory and method. In particular, the experiments clearly showed the advantages of the DISP-ET chip: portability, visuality, green use, rapidity, and flexibility for real-life use. Finally, the device was applied for a portable and visual assay of fresh milk from a cow on a dairy farm. The DISP-ET model opens a window for designing portable and visual quantitative methods of food-safety control and clinical diagnoses.
Magnetostrictive linear position sensors (MLPS) are high-precision sensors used in the industrial field for measuring the propagation time of ultrasonic signals in a waveguide. To date, MLPS have attracted widespread attention for their accuracy, reliability, and cost-efficiency in performing non-contact, multiple measurements. However, the sensor, with its traditional structure, is susceptible to electromagnetic interference, which affects accuracy. In the present study, we propose a novel structure of MLPS that relies on two differential waveguides to improve the signal-to-noise ratio, common-mode rejection ratio, and accuracy of MLPS. The proposed sensor model can depict sensor performance and the relationship of sensor parameters. Experimental results with the new sensor indicate that the new structure can improve accuracy to ±0.1 mm higher than ±0.2 mm with a traditional structure. In addition, the proposed sensor shows a considerable improvement in temperature characteristics.
A novel three-point method using a grating eddy current absolute position sensor (GECS) for bridge deflection estimation is proposed in this paper. Real spatial positions of the measuring points along the span axis are directly used as relative reference points of each other rather than using any other auxiliary static reference points for measuring devices in a conventional method. Every three adjacent measuring points are defined as a measuring unit and a straight connecting bar with a GECS fixed on the center section of it links the two endpoints. In each measuring unit, the displacement of the mid-measuring point relative to the connecting bar measured by the GECS is defined as the relative deflection. Absolute deflections of each measuring point can be calculated from the relative deflections of all the measuring units directly without any correcting approaches. Principles of the three-point method and displacement measurement of the GECS are introduced in detail. Both static and dynamic experiments have been carried out on a simple beam bridge model, which demonstrate that the three-point deflection estimation method using the GECS is effective and offers a reliable way for bridge deflection estimation, especially for long-term monitoring.