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Kosakonia cowanii (syn. Enterobacter cowanii) is a highly competitive bacterium that lives with plant, insect, fish, bird, and human organisms. It is pathogenic on some plants and an opportunistic pathogen of human. Nine novel viruses that lyse plant pathogenic strains and/or human strains of K. cowanii were isolated, sequenced, and characterized. Kc166A is a novel kayfunavirus, Kc261 is a novel bonnellvirus, and Kc318 is a new cronosvirus (all Autographiviridae). Kc237 is a new sortsnevirus, but Kc166B and Kc283 are members of new genera within Podoviridae. Kc304 is a new winklervirus, and Kc263 and Kc305 are new myoviruses. The viruses differ in host specificity, plaque phenotype, and lysis kinetics. Some of them should be suitable also as pathogen control agents.
Karel Petrzik; Sára Brázdová; Krzysztof Krawczyk. Novel Viruses That Lyse Plant and Human Strains of Kosakonia cowanii. Viruses 2021, 13, 1418 .
AMA StyleKarel Petrzik, Sára Brázdová, Krzysztof Krawczyk. Novel Viruses That Lyse Plant and Human Strains of Kosakonia cowanii. Viruses. 2021; 13 (8):1418.
Chicago/Turabian StyleKarel Petrzik; Sára Brázdová; Krzysztof Krawczyk. 2021. "Novel Viruses That Lyse Plant and Human Strains of Kosakonia cowanii." Viruses 13, no. 8: 1418.
Two novel dsDNA bacteriophages named Pectobacterium virus CB251 (PcCB251) and Pectobacterium virus CB7V (PcCB7V) targeting plant pathogen Pectobacterium parmentieri have been isolated and sequenced. The PcCB251 genome consists of 40,557 bp with G+C content of 48.6% and contains 47 predicted genes on a single strand. The phage is classified in genus Berlinvirus, family Autographiviridae. The PcCB7V phage has a circular dsDNA genome of 146,054 bp with G+C content of 50.4% and contains 269 predicted protein genes on both strands and 13 tRNA genes. The PcCB7V phage can be classified in genus Certrevirus, subfamily Vequintavirinae. Both novel bacteriophages have narrow host ranges, but they extend the list of candidates for phage-based control of pectolytic bacteria causing soft rot disease of potato.
Karel Petrzik; Martin Kmoch; Sára Brázdová; Rudolf Ševčík. Complete genome sequences of novel Berlinvirus and novel Certrevirus lytic for Pectobacterium sp. causing soft rot and black leg disease of potato. Virus Genes 2021, 57, 302 -305.
AMA StyleKarel Petrzik, Martin Kmoch, Sára Brázdová, Rudolf Ševčík. Complete genome sequences of novel Berlinvirus and novel Certrevirus lytic for Pectobacterium sp. causing soft rot and black leg disease of potato. Virus Genes. 2021; 57 (3):302-305.
Chicago/Turabian StyleKarel Petrzik; Martin Kmoch; Sára Brázdová; Rudolf Ševčík. 2021. "Complete genome sequences of novel Berlinvirus and novel Certrevirus lytic for Pectobacterium sp. causing soft rot and black leg disease of potato." Virus Genes 57, no. 3: 302-305.
Seven novel tailed lytic viruses (Ds3CZ, Ds5CZ, Ds9CZ, Ds16CZ, Ds20CZ, Ds23CZ, Ds25CZ) infecting the bacterium Dickeya solani were isolated in the Czech Republic. Genomes of these viruses are dsDNA, 149,364 to 155,285 bp in length, and the genome arrangement is very similar to that of the type virus Dickeya virus LIMEstone 1. All but the Ds25CZ virus should be regarded as strains of a single species. Most of the sequence differences are due to the presence or absence of homing endonuclease (HE) genes, with 23 HEs found in Ds3CZ, Ds5CZ, and Ds20CZ, 22 in Ds9CZ, 19 in Ds16CZ, 18 in Ds25CZ, and 15 in Ds23CZ.
Karel Petrzik; Josef Vacek; Sára Brázdová; Rudolf Ševčík; Igor Koloniuk. Diversity of limestone bacteriophages infecting Dickeya solani isolated in the Czech Republic. Archives of Virology 2021, 166, 1171 -1175.
AMA StyleKarel Petrzik, Josef Vacek, Sára Brázdová, Rudolf Ševčík, Igor Koloniuk. Diversity of limestone bacteriophages infecting Dickeya solani isolated in the Czech Republic. Archives of Virology. 2021; 166 (4):1171-1175.
Chicago/Turabian StyleKarel Petrzik; Josef Vacek; Sára Brázdová; Rudolf Ševčík; Igor Koloniuk. 2021. "Diversity of limestone bacteriophages infecting Dickeya solani isolated in the Czech Republic." Archives of Virology 166, no. 4: 1171-1175.
Widely distributed in water environments and in soil, cyanobacteria are hosts of lysogenic or lytic bacterioviruses. A novel, probably lysogenic virus (phage) for which the name Arthronema africanum virus TR020 (Aa-TR020) is proposed, has been isolated from filamentous freshwater cyanobacterium Arthronema africanum. The virus formed turbid plaques on plate culture of A. africanum strain 1980/01 but not on other Arthronema strain and other bacterial species. The genome of Aa-TR020 is linear molecule of dsDNA, 44,805 bp in length with 216 bp long terminal repeats and with G + C content of 46%. Fifty-five genes organized on plus and minus strands were predicted there. The genome size, gene arrangement, and selected protein sequences showed relatedness to Phormidium virus Pf-WMP3 and other viruses known to infect cyanobacteria and classified in the family Podoviridae.
Karel Petrzik; Jaromír Lukavský; Igor Koloniuk. Novel Virus on Filamentous Arthronema africanum Cyanobacterium. Microbial Ecology 2020, 81, 454 -459.
AMA StyleKarel Petrzik, Jaromír Lukavský, Igor Koloniuk. Novel Virus on Filamentous Arthronema africanum Cyanobacterium. Microbial Ecology. 2020; 81 (2):454-459.
Chicago/Turabian StyleKarel Petrzik; Jaromír Lukavský; Igor Koloniuk. 2020. "Novel Virus on Filamentous Arthronema africanum Cyanobacterium." Microbial Ecology 81, no. 2: 454-459.
We present a taxonomic proposal for revision of the family Secoviridae, a taxon of plant viruses in the order Picornavirales. We propose the reorganization of the genus Sadwavirus to create three new subgenera and to update the classification of five existing species. The proposed subgenera are "Satsumavirus" (one species: Satsuma dwarf virus), "Stramovirus" (two species: Strawberry mottle virus and Black raspberry necrosis virus) and "Cholivirus" (two species: Chocolate lily virus A and Dioscorea mosaic associated virus).
Hélène Sanfaçon; Indranil Dasgupta; Marc Fuchs; Alexander V. Karasev; Karel Petrzik; Jeremy R. Thompson; Ioannis Tzanetakis; René Van Der Vlugt; Thierry Wetzel; Nobuyuki Yoshikawa. Proposed revision of the family Secoviridae taxonomy to create three subgenera, “Satsumavirus”, “Stramovirus” and “Cholivirus”, in the genus Sadwavirus. Archives of Virology 2019, 165, 527 -533.
AMA StyleHélène Sanfaçon, Indranil Dasgupta, Marc Fuchs, Alexander V. Karasev, Karel Petrzik, Jeremy R. Thompson, Ioannis Tzanetakis, René Van Der Vlugt, Thierry Wetzel, Nobuyuki Yoshikawa. Proposed revision of the family Secoviridae taxonomy to create three subgenera, “Satsumavirus”, “Stramovirus” and “Cholivirus”, in the genus Sadwavirus. Archives of Virology. 2019; 165 (2):527-533.
Chicago/Turabian StyleHélène Sanfaçon; Indranil Dasgupta; Marc Fuchs; Alexander V. Karasev; Karel Petrzik; Jeremy R. Thompson; Ioannis Tzanetakis; René Van Der Vlugt; Thierry Wetzel; Nobuyuki Yoshikawa. 2019. "Proposed revision of the family Secoviridae taxonomy to create three subgenera, “Satsumavirus”, “Stramovirus” and “Cholivirus”, in the genus Sadwavirus." Archives of Virology 165, no. 2: 527-533.
A lichen body is formed most often from green alga cells trapped in a net of ascomycetous fungi and accompanied by endolichenic or parasitic fungi, other algae, and symbiotic or free-living bacteria. The lichen’s microcosmos is inhabited by mites, insects, and other animals for which the lichen is a source of food or a place to live. Novel, four-segmented dsRNA viruses were detected in saxicolous Chrysothrix chlorina and Lepraria incana lichens. Comparison of encoded genome proteins revealed classification of the viruses to the genus Alphachrysovirus and a relationship to chrysoviruses from filamentous ascomycetous fungi. We propose the names Chrysothrix chrysovirus 1 (CcCV1) and Lepraria chrysovirus 1 (LiCV1) as acronyms for these viruses. Surprisingly, observation of Chrysothrix chlorina hybridization with fluorescent-labelled virus probe by confocal microscope revealed that the CcCV1 virus is not present in the lichen body-forming fungus but in accompanying endolichenic Penicillium citreosulfuratum fungus. These are the first descriptions of mycoviruses from a lichen environment.
Karel Petrzik; Igor Koloniuk; Hana Sehadová; Tatiana Sarkisova. Chrysoviruses Inhabited Symbiotic Fungi of Lichens. Viruses 2019, 11, 1120 .
AMA StyleKarel Petrzik, Igor Koloniuk, Hana Sehadová, Tatiana Sarkisova. Chrysoviruses Inhabited Symbiotic Fungi of Lichens. Viruses. 2019; 11 (12):1120.
Chicago/Turabian StyleKarel Petrzik; Igor Koloniuk; Hana Sehadová; Tatiana Sarkisova. 2019. "Chrysoviruses Inhabited Symbiotic Fungi of Lichens." Viruses 11, no. 12: 1120.
A novel bisegmented double-stranded RNA virus was identified in the mycoparasitic and opportunistic fungus Hypomyces chrysospermus. The RNA1 genome segment comprises 1866 bp and encodes an RNA-dependent RNA polymerase (RdRp). The RNA2 segment comprises 1822 bp and encodes a capsid protein. Phylogenetic analysis of the RdRp protein indicated that this virus is a new member of genus Alphapartitivirus in the family Partitiviridae. We have designated this mycovirus as “Hypomyces chrysospermus partitivirus 1” (HcPV1). HcPV1 is highly transmissible with aleurioconidia and is present in large amounts within growing mycelium in comparison to the GAPDH reference gene.
Karel Petrzik; Abu Bakar Siddique. A mycoparasitic and opportunistic fungus is inhabited by a mycovirus. Archives of Virology 2019, 164, 2545 -2549.
AMA StyleKarel Petrzik, Abu Bakar Siddique. A mycoparasitic and opportunistic fungus is inhabited by a mycovirus. Archives of Virology. 2019; 164 (10):2545-2549.
Chicago/Turabian StyleKarel Petrzik; Abu Bakar Siddique. 2019. "A mycoparasitic and opportunistic fungus is inhabited by a mycovirus." Archives of Virology 164, no. 10: 2545-2549.
The family Partitiviridae consists of dsRNA viruses with genome separated into two segments and encoding replicase and capsid protein only. We examined the nucleotide diversity expressed as the ratio dN/dS of nonsynonymous and synonymous substitutions, which has been calculated for 12 representative viruses of all five genera of partitiviruses. We can state that strong purifying selection works on both the RdRp and CP genes and propose that putative positive selection occurs also on the RdRp genes in two viruses. Among the 95 evaluated viruses, wherein both segments had been sequenced, 8 viruses in betapartitiviruses and 9 in alphapartitiviruses were identified as reassortment candidates because they differ extremely in their CP identity even as they are related in terms of RdRp. Furthermore, there are indications that reassortants are present among isolates of different viruses.
Karel Petrzik. Evolutionary forces at work in partitiviruses. Virus Genes 2019, 55, 563 -573.
AMA StyleKarel Petrzik. Evolutionary forces at work in partitiviruses. Virus Genes. 2019; 55 (5):563-573.
Chicago/Turabian StyleKarel Petrzik. 2019. "Evolutionary forces at work in partitiviruses." Virus Genes 55, no. 5: 563-573.
During their lifetime, perennial woody plants are expected to face multiple infection events. Furthermore, multiple genotypes of individual virus species may co-infect the same host. This may eventually lead to a situation where plants harbor complex communities of viral species/strains. Using high-throughput sequencing, we describe co-infection of sweet and sour cherry trees with diverse genomic variants of two closely related viruses, namely prunus virus F (PrVF) and cherry virus F (CVF). Both viruses are most homologous to members of the Fabavirus genus (Secoviridae family). The comparison of CVF and PrVF RNA2 genomic sequences suggests that the two viruses may significantly differ in their expression strategy. Indeed, similar to comoviruses, the smaller genomic segment of PrVF, RNA2, may be translated in two collinear proteins while CVF likely expresses only the shorter of these two proteins. Linked with the observation that identity levels between the coat proteins of these two viruses are significantly below the family species demarcation cut-off, these findings support the idea that CVF and PrVF represent two separate Fabavirus species.
Igor Koloniuk; Tatiana Sarkisova; Karel Petrzik; Ondřej Lenz; Jaroslava Přibylová; Jana Fránová; Josef Špak; Leonidas Lotos; Christina Beta; Asimina Katsiani; Thierry Candresse; Varvara I. Maliogka. Variability Studies of Two Prunus-Infecting Fabaviruses with the Aid of High-Throughput Sequencing. Viruses 2018, 10, 204 .
AMA StyleIgor Koloniuk, Tatiana Sarkisova, Karel Petrzik, Ondřej Lenz, Jaroslava Přibylová, Jana Fránová, Josef Špak, Leonidas Lotos, Christina Beta, Asimina Katsiani, Thierry Candresse, Varvara I. Maliogka. Variability Studies of Two Prunus-Infecting Fabaviruses with the Aid of High-Throughput Sequencing. Viruses. 2018; 10 (4):204.
Chicago/Turabian StyleIgor Koloniuk; Tatiana Sarkisova; Karel Petrzik; Ondřej Lenz; Jaroslava Přibylová; Jana Fránová; Josef Špak; Leonidas Lotos; Christina Beta; Asimina Katsiani; Thierry Candresse; Varvara I. Maliogka. 2018. "Variability Studies of Two Prunus-Infecting Fabaviruses with the Aid of High-Throughput Sequencing." Viruses 10, no. 4: 204.
During aetiological study of diseased red clover (Trifolium pratense L.) using high throughput sequencing, a novel virus with a 10 kb genome divided into two segments was discovered. The virus, tentatively named red clover associated varicosavirus (RCaVV), is phylogenetically related to classifiable members of the genus Varicosavirus (family Rhabdoviridae, order Mononegavirales). Analysis of mRNA levels from the individual RCaVV genes suggested possible differences in transcription regulation between rhabdoviruses with divided and undivided genomes.
Igor Koloniuk; Jana Fránová; Tatiana Sarkisova; Jaroslava Přibylová; Ondřej Lenz; Karel Petrzik; Josef Špak. Identification and molecular characterization of a novel varicosa-like virus from red clover. Archives of Virology 2018, 163, 2213 -2218.
AMA StyleIgor Koloniuk, Jana Fránová, Tatiana Sarkisova, Jaroslava Přibylová, Ondřej Lenz, Karel Petrzik, Josef Špak. Identification and molecular characterization of a novel varicosa-like virus from red clover. Archives of Virology. 2018; 163 (8):2213-2218.
Chicago/Turabian StyleIgor Koloniuk; Jana Fránová; Tatiana Sarkisova; Jaroslava Přibylová; Ondřej Lenz; Karel Petrzik; Josef Špak. 2018. "Identification and molecular characterization of a novel varicosa-like virus from red clover." Archives of Virology 163, no. 8: 2213-2218.
Mixed infection by three dsRNA viruses, a novel betapartitivirus, a gammapartitivirus, and a novel fusarivirus, has been identified in four isolates of the obligate alkalophilic fungus Sodiomyces alkalinus. The first, Sodiomyces alkalinus partitivirus 1 (SaPV1), is placed within the genus Betapartitivirus and is related to Ustilaginoidea virens partitivirus 2. The taxonomic position of the second virus is less clear as it shares high (85%) amino acid sequence identity but significantly low (77%) nucleotide sequence identity of the capsid protein with Colletotrichum truncatum partitivirus 1. The third, the novel Sodiomyces alkalinus fusarivirus 1 (SaFV1), is related to Fusarium poae fusarivirus 1. All the viruses show efficient vertical transmission through asexual and sexual spores. These novel coexisting viruses do not evoke apparent phenotypic alteration to their fungal host. This is the first description of a viral infection in an alkalophilic fungus.
Lenka Hrabáková; Alexey Grum-Grzhimaylo; Igor Koloniuk; Alfons J. M. Debets; Tatiana Sarkisova; Karel Petrzik. The alkalophilic fungus Sodiomyces alkalinus hosts beta- and gammapartitiviruses together with a new fusarivirus. PLOS ONE 2017, 12, e0187799 -e0187799.
AMA StyleLenka Hrabáková, Alexey Grum-Grzhimaylo, Igor Koloniuk, Alfons J. M. Debets, Tatiana Sarkisova, Karel Petrzik. The alkalophilic fungus Sodiomyces alkalinus hosts beta- and gammapartitiviruses together with a new fusarivirus. PLOS ONE. 2017; 12 (11):e0187799-e0187799.
Chicago/Turabian StyleLenka Hrabáková; Alexey Grum-Grzhimaylo; Igor Koloniuk; Alfons J. M. Debets; Tatiana Sarkisova; Karel Petrzik. 2017. "The alkalophilic fungus Sodiomyces alkalinus hosts beta- and gammapartitiviruses together with a new fusarivirus." PLOS ONE 12, no. 11: e0187799-e0187799.
The complete nucleotide sequence of a new RNA mycovirus in the KY isolate of Phomopsis longicolla Hobbs 1985 and its protoplasts subcultures p5, p9, and ME711 was discovered. The virus, provisionally named Phomopsis longicolla RNA virus 1 (PlRV1), was localized in mitochondria and was determined to have a genome 2822 nucleotides long. A single open reading frame could be translated in silico by both standard and mitochondrial genetic codes into a product featuring conservative domains for an RNA-dependent RNA polymerase (RdRp). The RdRp of PlRV1 has no counterpart among mycoviruses, but it is about 30% identical with the RdRp of plant ourmiaviruses. Recently, new mycoviruses related to plant ourmiaviruses and forming one clade with PlRV1 have been discovered. This separate clade could represent the crucial link between plant and fungal viruses.
Lenka Hrabáková; Igor Koloniuk; Karel Petrzik. Phomopsis longicolla RNA virus 1 – Novel virus at the edge of myco- and plant viruses. Virology 2017, 506, 14 -18.
AMA StyleLenka Hrabáková, Igor Koloniuk, Karel Petrzik. Phomopsis longicolla RNA virus 1 – Novel virus at the edge of myco- and plant viruses. Virology. 2017; 506 ():14-18.
Chicago/Turabian StyleLenka Hrabáková; Igor Koloniuk; Karel Petrzik. 2017. "Phomopsis longicolla RNA virus 1 – Novel virus at the edge of myco- and plant viruses." Virology 506, no. : 14-18.
A new bisegmented dsRNA virus has been detected in shoots of blue spruce with bud blight disease symptoms and infection by Cucurbitaria piceae (Bortw.) fungus. The virus genome consists of two segments that are 2071 and 2257 nt long, encoding the putative RNA polymerase and capsid protein, respectively. Rosellinia necatrix partitivirus 1 is a closely related virus with 45 % amino acid sequence identity in the polymerase, and crimson clover cryptic virus 2 has 36 % amino acid sequence identity in the capsid protein. Based on taxonomic criteria, the virus should be classified as a member of the genus Betapartitivirus (family Partitiviridae). The name Cucurbitaria piceae partitivirus 1 and acronym CpPV1 are proposed.
Karel Petrzik; Igor Koloniuk; Tatiana Sarkisova; Lenka Hrabáková. Detection and genome sequence of a new betapartitivirus associated with Cucurbitaria piceae Borthw. fungus causing bud blight of spruce in the Czech Republic. Archives of Virology 2016, 161, 1405 -1409.
AMA StyleKarel Petrzik, Igor Koloniuk, Tatiana Sarkisova, Lenka Hrabáková. Detection and genome sequence of a new betapartitivirus associated with Cucurbitaria piceae Borthw. fungus causing bud blight of spruce in the Czech Republic. Archives of Virology. 2016; 161 (5):1405-1409.
Chicago/Turabian StyleKarel Petrzik; Igor Koloniuk; Tatiana Sarkisova; Lenka Hrabáková. 2016. "Detection and genome sequence of a new betapartitivirus associated with Cucurbitaria piceae Borthw. fungus causing bud blight of spruce in the Czech Republic." Archives of Virology 161, no. 5: 1405-1409.
A novel dsRNA virus was identified in the mycorrhizal fungus Thelephora terrestris (Ehrh.) and sequenced. This virus, named Thelephora terrestris virus 1 (TtV1), contains two reading frames in different frames but with the possibility that ORF2 could be translated as a fusion polyprotein after ribosomal -1 frameshifting. Picornavirus 2A-like motif, nudix hydrolase, phytoreovirus S7, and RdRp domains were found in a unique arrangement on the polyprotein. A new genus named Phlegivirus and containing TtV1, PgLV1, RfV1 and LeV is therefore proposed. Twenty species of oribatid mites were identified in soil material in the vicinity of T. terrestris. TtV1 was detected in large amounts in Steganacarus (Tropacarus) carinatus (C.L. Koch, 1841) and in much smaller amounts in Nothrus silvestris (Nicolet). This is the first description of mycovirus presence in oribatid mites
Karel Petrzik; Tatiana Sarkisova; Josef Starý; Igor Koloniuk; Lenka Hrabáková; Olga Kubešová. Molecular characterization of a new monopartite dsRNA mycovirus from mycorrhizal Thelephora terrestris (Ehrh.) and its detection in soil oribatid mites (Acari: Oribatida). Virology 2016, 489, 12 -19.
AMA StyleKarel Petrzik, Tatiana Sarkisova, Josef Starý, Igor Koloniuk, Lenka Hrabáková, Olga Kubešová. Molecular characterization of a new monopartite dsRNA mycovirus from mycorrhizal Thelephora terrestris (Ehrh.) and its detection in soil oribatid mites (Acari: Oribatida). Virology. 2016; 489 ():12-19.
Chicago/Turabian StyleKarel Petrzik; Tatiana Sarkisova; Josef Starý; Igor Koloniuk; Lenka Hrabáková; Olga Kubešová. 2016. "Molecular characterization of a new monopartite dsRNA mycovirus from mycorrhizal Thelephora terrestris (Ehrh.) and its detection in soil oribatid mites (Acari: Oribatida)." Virology 489, no. : 12-19.
The complete nucleotide sequence of a novel virus from red currant, provisionally named currant virus A (CuVA), was determined. The genome is 7925 nucleotides long and has a 3'-poly(A) tail. The genome organization with two overlapping open reading frames is similar to that of capilloviruses, but the CuVA genome is about 600 nucleotides longer than that of the longest known capillovirus, cherry virus A. The RNA is predicted to encode a polyprotein with domains of methyltransferase, 2OG-Fe(II) oxygenase, papain-like protease, RNA helicase, RdRp, and capsid protein. Phylogenetic analysis confirms that CuVA is a new and distinct member of the genus Capillovirus.
Karel Petrzik; Jaroslava Přibylová; Igor Koloniuk; Josef Špak. Molecular characterization of a novel capillovirus from red currant. Archives of Virology 2016, 161, 1083 -1086.
AMA StyleKarel Petrzik, Jaroslava Přibylová, Igor Koloniuk, Josef Špak. Molecular characterization of a novel capillovirus from red currant. Archives of Virology. 2016; 161 (4):1083-1086.
Chicago/Turabian StyleKarel Petrzik; Jaroslava Přibylová; Igor Koloniuk; Josef Špak. 2016. "Molecular characterization of a novel capillovirus from red currant." Archives of Virology 161, no. 4: 1083-1086.
The complete nucleotide sequences of RNA1 and RNA2 of the Holandský červený strain of currant latent virus (CuLV) were determined using next-generation sequencing. The RNA1 is predicted to encode a polyprotein 2124 amino acid long with RdRp motifs. The RNA2 is predicted to encode a polyprotein 957 amino acid long with homology to the capsid protein of apple latent spherical virus and cherry rasp leaf virus. Phylogenetic analysis confirms that CuLV is a new distinct member of the genus Cheravirus.
Karel Petrzik; Igor Koloniuk; Jaroslava Přibylová; Josef Špak. Complete genome sequence of currant latent virus (genus Cheravirus, family Secoviridae). Archives of Virology 2015, 161, 491 -493.
AMA StyleKarel Petrzik, Igor Koloniuk, Jaroslava Přibylová, Josef Špak. Complete genome sequence of currant latent virus (genus Cheravirus, family Secoviridae). Archives of Virology. 2015; 161 (2):491-493.
Chicago/Turabian StyleKarel Petrzik; Igor Koloniuk; Jaroslava Přibylová; Josef Špak. 2015. "Complete genome sequence of currant latent virus (genus Cheravirus, family Secoviridae)." Archives of Virology 161, no. 2: 491-493.
Igor Koloniuk; Lenka Hrabáková; Karel Petrzik. Addendum to: Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana. Archives of Virology 2015, 160, 2139 -2139.
AMA StyleIgor Koloniuk, Lenka Hrabáková, Karel Petrzik. Addendum to: Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana. Archives of Virology. 2015; 160 (8):2139-2139.
Chicago/Turabian StyleIgor Koloniuk; Lenka Hrabáková; Karel Petrzik. 2015. "Addendum to: Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana." Archives of Virology 160, no. 8: 2139-2139.
Beauveria bassiana is a ubiquitous entomopathogen infecting hundreds of insect species. We have determined the genomic organization and the complete nucleotide sequence of a novel virus isolated from the isolate A24 of B. bassiana. Phylogenetic analysis of the polymerase gene reveals that the virus, tentatively named Beauveria bassiana virus 1, belongs to the family Amalgaviridae and represents a distinct lineage of amalgaviruses infecting fungi.
Igor Koloniuk; Lenka Hrabáková; Karel Petrzik. Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana. Archives of Virology 2015, 160, 1585 -1588.
AMA StyleIgor Koloniuk, Lenka Hrabáková, Karel Petrzik. Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana. Archives of Virology. 2015; 160 (6):1585-1588.
Chicago/Turabian StyleIgor Koloniuk; Lenka Hrabáková; Karel Petrzik. 2015. "Molecular characterization of a novel amalgavirus from the entomopathogenic fungus Beauveria bassiana." Archives of Virology 160, no. 6: 1585-1588.
Trebouxia aggregata (Archibald) Gärtner (phylum Chlorophyta, family Trebouxiaceae), a lichen symbiotic alga, has been identified as host of the well-known herbaceous plant virus Cauliflower mosaic virus (CaMV, family Caulimoviridae). The alga had been isolated from Xanthoria parietina more than 70 years ago and has been maintained in a collection since that time. The CaMV detected in this collection entry has now been completely sequenced. The virus from T. aggregata is mechanically transmissible to a herbaceous host and induces disease symptoms there. Its genome differs by 173 nt from the closest European CaMV-D/H isolate from cauliflower. No site under positive selection was found on the CaMV genome from T. aggregata. We therefore assume that the virus’s presence in this alga was not sufficiently long to fix any specific changes in its genome. Apart from this symbiotic alga, CaMV capsid protein sequences were amplified from many other non-symbiotic algae species maintained in a collection (e.g., Oonephris obesa, Elliptochloris sp., Microthamnion kuetzingianum, Chlorella vulgaris, Pseudococcomyxa sp.). CaMV-free Chlorella vulgaris was treated with CaMV to establish virus infection. The virus was still detected there after five passages. The virus infection is morphologically symptomless on Chlorella algae and the photosynthesis activity is slightly decreased in comparison to CaMV-free alga culture. This is the first proof as to the natural presence of CaMV in algae and the first demonstration of algae being artificially infected with this virus.
Karel Petrzik; Jan Vondrák; Jana Kvíderová; Jaromír Lukavský. Platinum Anniversary: Virus and Lichen Alga Together More than 70 Years. PLOS ONE 2015, 10, e0120768 .
AMA StyleKarel Petrzik, Jan Vondrák, Jana Kvíderová, Jaromír Lukavský. Platinum Anniversary: Virus and Lichen Alga Together More than 70 Years. PLOS ONE. 2015; 10 (3):e0120768.
Chicago/Turabian StyleKarel Petrzik; Jan Vondrák; Jana Kvíderová; Jaromír Lukavský. 2015. "Platinum Anniversary: Virus and Lichen Alga Together More than 70 Years." PLOS ONE 10, no. 3: e0120768.
A new virus affecting currants was detected in the Czech Republic in several cultivars of black, red and white currants and in a currant colonized by the aphid Cryptomyzus ribis. The infection was not associated with any symptoms on the plants. The virus has a divided genome consisting of two single-stranded, polyadenylated RNAs, and partial sequences of RNA1 and RNA2 indicate that it is closely related to Cherry rasp leaf virus and Apple latent spherical virus (Secoviridae). It is regarded as a new member in the genus Cheravirus, and the name Currant latent virus (CuLV) is proposed.
Karel Petrzik; Jaroslava Přibylová; Josef Špak; Jan Havelka. Partial genome sequence of currant latent virus, a new chera-like virus related to Apple latent spherical virus. Journal of General Plant Pathology 2015, 81, 142 -145.
AMA StyleKarel Petrzik, Jaroslava Přibylová, Josef Špak, Jan Havelka. Partial genome sequence of currant latent virus, a new chera-like virus related to Apple latent spherical virus. Journal of General Plant Pathology. 2015; 81 (2):142-145.
Chicago/Turabian StyleKarel Petrzik; Jaroslava Přibylová; Josef Špak; Jan Havelka. 2015. "Partial genome sequence of currant latent virus, a new chera-like virus related to Apple latent spherical virus." Journal of General Plant Pathology 81, no. 2: 142-145.