Serpa cheese, a Portuguese Protected of Denomination Origin (PDO) cheese, known for its unique sensory attributes, is made from the raw milk of native sheep. In this preliminary work, ten samples of Serpa cheese were submitted for a sensory evaluation performed by an expert panel in a sensory laboratory accredited according to ISO 17025 for Serpa cheese parameters, and the panelists classified the cheeses based on texture, taste and odor scores, in accordance with the specifications for the classification of this type of cheese. All cheeses were analyzed by SPME-GC-MS. Following an exploratory unsupervised multivariate analysis, the supervised multivariate analysis by partial least squares—discriminant analysis (PLS-DA), associated the relative percent area of the identified volatiles with the classification of cheeses attributed by the sensory panel. Among the 144 compounds putatively identified, there was a pattern of compound distribution of some of them, such as acetoin, diacetyl, and 2,3-butanediol, leaning toward the cheese samples with high taste and odor scores, while other compounds, such as ethyl caprate, capric acid, and 3-methylindole, were more associated with the cheese samples rated with a low score. Despite the reduced number of samples that may have imposed some restrictions on the conclusions drawn, there was a clear trend in the volatiles’ distribution, allowing us to identify, based on the higher correlation loadings, potential candidates for the Serpa cheese sensory quality. This preliminary study presents, for the first time, an overview of the volatiles that are present in Serpa PDO cheese that may be responsible for the positive or negative sensory evaluation of this PDO cheese.

Under the principles of the circular economy and sustainability, consumers, the food industry and health authorities have interest in new natural food preservatives to prevent foodborne diseases and increase produce shelf life. This work aimed to evaluate the antimicrobial properties of cowpea plant extracts. Grain, pod and leaf extracts from five Portuguese cowpea accessions were characterized in terms of their phenolic content. The values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined against pathogenic and non-pathogenic bacteria, as well as on post-harvest pathogenic filamentous fungi. In general, the phenolic content of pod extracts was higher than that of grains and leaves, although leaf extracts had the highest content of a broad-spectrum antibacterial flavonoid, quercetin. Grain extracts exhibited no detectable antimicrobial activity. In contrast, leaf and pod extracts from all five accessions generally displayed bactericidal effects. For bacteria, pod extracts showed MICs ranging from 5.1 to 87.7 mg/mL and MBCs from 20.3 to 87.7 mg/mL. Leaf extracts showed the most promising results, with MICs and MBCs ranging from 1.1 to 9.1 mg/mL. The results against fungi were not so expressive; nevertheless, P. expansum was inhibited by 9 L leaf extract even if at a higher concentration (MIC = 18 mg/mL) than those obtained for bacteria. The Portuguese variety Fradel (1E) showed very promising antibacterial activity, with leaf extracts showing low MBC values (from 2.3 to 9.1 mg/mL). The obtained results indicate that cowpea pods and leaves have antimicrobial properties and could potentially be used as a source of compounds for food preservation.