Future climate change will have serious impacts on species survival and distribution and will likely lead to the extinction of some species classified as endangered. Carpinus tientaiensis (Betulaceae), a unique and endangered species in China, has restricted distribution and a small population, indicating an urgent need for its protection. However, research on its current distribution or the influence that climate change will have on its future survival and distribution is limited. We used a MaxEnt model and ArcGIS software to predict the current and future niches of C. tientaiensis. The current suitable distribution area of C. tientaiensis is small, mainly in east China, south Zhejiang and Anhui, and central and southern mountainous areas of Taiwan province. The core suitable areas are concentrated in the Xianxialing and Kuocang mountains in south Zhejiang, the southern mountains of Taiwan, and the Dabie, Huangshan and Jiuhua mountains in south Anhui. Among the 15 BIOCLIM variables examined, the precipitation of the driest quarter (bio17) was found to be the most important factor limiting C. tientaiensis survival and distribution. Future field investigations will focus on the Xianxialing and Kuocang mountains, as they may have unidentified wild C. tientaiensis communities. In the future, the Kuocang, Dapan and Tiantai mountains in east Zhejiang, and the high-altitude areas of Dabie and Jiuhua mountains in south Anhui, will be suitable for C. tientaiensis ex situ conservation and cultivation. However, the suitable distribution and core suitable areas for C. tientaiensis will decrease sharply as they are susceptible to climate shocks. Moreover, the suitable distribution area of C. tientaiensis is predicted to move slightly north and obviously eastward. Therefore, we suggest that strengthen conservation and management efforts for C. tientaiensis in its original habitats, and actively carry out ex situ conservation and artificial breeding in botanical gardens.

Rapid urbanization and increasing land scarcity have made urban agriculture and efficient space utilization critical directions in modern agriculture. Ougan, a fruit tree valued for both its economic and ecological benefits, holds significant promise for dwarfing cultivation techniques. In this study, a root-irrigation method was used to apply paclobutrazol at various concentrations (200, 500, 1000, 1500, and 2000 mg/L) to Ougan seedlings, with a control group for comparison. Growth parameters include an average daily increase of plant height, stem girth, new branches, and new branch girth, as well as physiological indices such as leaf SPAD values, leaf nitrogen content, net photosynthetic rate, stomatal conductance, intercellular CO₂ concentration, and transpiration rate, were measured during both spring and summer growth periods. The results demonstrate that PBZ exerts a distinct concentration-dependent regulatory effect on Ougan growth: higher concentrations significantly inhibited plant height while promoting increases in stem diameter, with several parameters exhibiting a unimodal response. Short-term (spring) PBZ application enhanced certain photosynthetic parameters, such as net photosynthetic rate and stomatal conductance; however, prolonged exposure (summer) resulted in a decline in photosynthetic efficiency and overall leaf physiological status. Through comprehensive evaluation using principal component analysis and PLS-SEM, the 500 mg/L PBZ treatment was identified as achieving the optimal balance between growth inhibition and the maintenance of photosynthetic and nutritional status, closely approximating the ideal dwarfing effect. This study elucidates the complex regulatory effects of PBZ on the growth, photosynthesis, and carbon assimilation of Ougan through natural climate, providing robust technical parameters and theoretical support for future dwarf cultivation practices. These findings facilitate the development of dwarf fruit trees into bonsai vegetation, demonstrating significant horticultural application potential.

Bingdao ancient tree tea (BATT), a type of raw Pu-erh tea, is renowned for its brewing durability, characterized by a unique aroma and flavor. To explore the dynamic changes in infusion quality and the impact of multiple steeping process, BATT was brewed 14 times, and its sensory attributes, infusion color, and chemical composition were assessed across different brewing intervals. The color of the tea infusion remained relatively stable throughout the brewing process. Sensory evaluation indicated that BATT exhibited optimal sensory quality between the third and seventh infusions. While the leaching of polyphenols showed minimal variation across brews, the concentrations of ester-catechins, non-ester catechins, free amino acids, and caffeine after the seventh brewing decreased by 28.82%, 21.83%, 28.86%, and 40.37%, respectively. Our results indicated that higher concentrations of flavor compounds in the BATT infusion appeared between the fourth and seventh brews. This study provides a theoretical basis for understanding the brewing characteristics of BATT.

Spreading is the key process for ensuring green tea quality. However, the effect of blue light intensity conditions on the formation of green tea aroma and the evolution of key volatile compounds has not been assessed to date. Four tea samples treated with different light conditions (blue light intensities) were used to investigate the effect of spreading treatment on changes in the composition and content of volatile compounds. Volatile compounds in green tea samples were detected using headspace-solid phase microextraction and gas chromatography-mass spectrometry under different light conditions. Orthogonal partial least squares discriminant analysis (OPLS-DA) and relative odor activity value (rOAV) analyses were then applied to clarify the best blue light condition for forming aroma and associated compounds. The 116 volatile compounds were detected in the green tea samples, of which alcohols were the most abundant. The findings demonstrated that MBL (middle-intensity blue light; 150 μmol/(m²∙s)) treatment was the most effective condition for developing an intense and persistent fruity and floral scent compared to HBL (high-intensity blue light; 300 μmol/(m²∙s)) and LBL (low-intensity blue light; 75 μmol/(m²∙s)). This study underscores how blue light intensity conditions shape green tea aromas and offers operational insights. It also provides a theoretical basis for controlling light conditions in the process of green tea spreading

Anthracnose is a leaf fungal disease caused by multiple Colletotrichum species. Currently, the predominant deployment of chemical agents for anthracnose control increases ecological pollution risks and potential food safety concerns. The comprehension of the pathogenic mechanism and physicochemical properties of anthracnose is, therefore, essential for effective prevention and control. In this study, the pathogenic strain (pathogen) was isolated from the infected tea plant (Camellia sinensis) leaves and was identified as Colletotrichum gloeosporioides based on microscope observations and gene sequences. This fungus exhibited optimal growth at 28 °C and a pH of 6, with a lethal temperature threshold of 53 °C on PDA plate medium. The 80% tea saponin and 10% polymycin B could effectively inhibit its mycelium growth. Notably, the 10% polyoxin B exhibits a stronger inhibitory effect with an EC₅₀ value of 1.07 mg mL⁻¹. Following infection with Colletotrichum gloeosporioides, the resistant cultivar ‘Zhongcha 108’ exhibited higher levels of H₂O₂ and O₂⁻ than the susceptible ‘Longjing 43’, with later symptom onset and slower disease progression. Although the exogenous treatment of methyl jasmonate (MeJA) did not inhibit C. gloeosporioides directly, it significantly reduced lesion areas in ‘Longjing 43’ leaves caused by C. gloeosporioides. This treatment increased peroxidase and superoxide dismutase activities, but limited malondialdehyde content, thereby enhancing ‘Longjing 43’ resistance to the pathogen. The findings provide scientific guidance for the anthracnose prevention and control in tea gardens.

Tea plants (Camellia sinensis) uniquely hyperaccumulate fluoride (F) and concurrently exhibit a preference for ammonium nitrogen (NH₄⁺-N) over nitrate nitrogen (NO₃⁻-N). However, the mechanistic basis for co-existence of NH₄⁺-N preference and F hyperaccumulation in C. sinensis remains unexplored. Here, we investigated F accumulation and translocation with varying N supplies (0 mM and 2.854 mM N with NH₄⁺-N:NO₃⁻-N ratios of 3:1, 4:0 and 0:4) and F concentrations (0, 8 and 16 mg·L⁻¹ NaF) to reveal the mechanism driving NH₄⁺-N preference and F hyperaccumulation in C. sinensis. Results show that NH₄⁺-N supply enhanced H⁺ efflux, mobilizing aluminum (Al) to form mobile Al-F complexes for translocation to shoots, thereby alleviating F toxicity in roots. This process was facilitated by transporters including CsCLCd, CsCLCe, CsCLCf2 and CsFEX. In contrast, NO₃⁻-N promoted root sequestration of F as immobile calcium (Ca)-F complexes, exacerbating damage. Under NO₃⁻-N supply, CsCLCb primarily mediated NO₃⁻ transport, while CsCLCc, CsCLCe, CsCLCf1, CsCLCf2 and CsFEX were involved in F transport. In leaves, CsCLCd, CsCLCe, CsCLCf1, CsCLCf2, CsCLCg and CsFEX mediated vacuolar sequestration under both N conditions. These findings elucidate that NH₄⁺-N preference is mechanistically linked to F hyperaccumulation through an Al-assisted translocation pathway, which confers tolerance by exporting F from roots.