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Zainol Haida
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, Malaysia

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Journal article
Published: 14 August 2020 in Plants
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Clinacanthus nutans, commonly known as Sabah snake grass, is one of the more important medicinal plants in Malaysia’s herbal industry. C. nutans has gained the attention of medical practitioners due to its wide range of bioactive compounds responsible for various biological activities, such as anti-cancer, anti-venom and anti-viral activities. Due to its high pharmacological properties, the species has been overexploited to meet the demands of the pharmaceutical industry. The present study was conducted to establish a suitable in vitro culture procedure for the mass propagation of C. nutans. Murashige and Skoog (MS) basal medium, supplemented with different types of cytokinins, auxins, basal medium strength and sucrose concentrations, were tested. Based on the results, a full-strength MS basal medium supplemented with 12 µM 6-benzylaminopurine (BAP) and 30 g/L sucrose was recorded as the best outcome for all the parameters measured including the regeneration percentage, number of shoots, length of shoots, number of leaves and fresh weight of leaves. In the analysis of the phenolics content and antioxidant activities, tissue-cultured leaf extracts assayed at 100 °C exhibited the highest phenolic content and antioxidant activities. The propagation of C. nutans via a plant tissue culture technique was recorded to be able to produce high phenolic contents as well as exhibit high antioxidant activities.

ACS Style

Zainol Haida; Jaafar Juju Nakasha; Mansor Hakiman. In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass). Plants 2020, 9, 1030 .

AMA Style

Zainol Haida, Jaafar Juju Nakasha, Mansor Hakiman. In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass). Plants. 2020; 9 (8):1030.

Chicago/Turabian Style

Zainol Haida; Jaafar Juju Nakasha; Mansor Hakiman. 2020. "In Vitro Responses of Plant Growth Factors on Growth, Yield, Phenolics Content and Antioxidant Activities of Clinacanthus nutans (Sabah Snake Grass)." Plants 9, no. 8: 1030.