The exponential increase in medical waste production has increased the difficulty of waste management, resulting in higher medical waste dispersion into the environment. By employing a circular economy approach, it is possible to develop new materials by waste valorization. The employment of biodegradable and renewable agro-food, waste-derived materials may reduce the environmental impact caused by the dispersion of medical waste. In this work, tomato peel recovered cutin was blended with poly(L-lactide-co-ε-caprolactone) (PLAPCL) to develop new textiles for medical application through electrospinning. The textile fabrication process was studied by varying Cut content in the starting suspensions and by optimizing fabrication parameters. Devices with dense and porous structures were developed, and their morphological, thermal, and physical–chemical properties were evaluated through scanning electron microscopy, differential scanning calorimetry, thermogravimetric analysis, and Fourier transformed infrared spectroscopy. Textile material stability to γ-irradiation was evaluated through gel permeation chromatography, while its wettability, mechanical properties, and biocompatibility were analyzed through contact angle measurement, tensile test, and MTT assay, respectively. The LCA methodology was used to evaluate the environmental impact of textile production, with a specific focus on greenhouse gas (GHG) emissions. The main results demonstrated the suitability of PLAPCL–cutin blends to be processed through electrospinning and the obtained textile’s suitability to be used to develop surgical face masks or patches for wound healing.

Agricultural waste derivatives, particularly tomato cutin, a biopolymer found in the cuticular layer of plants, present a promising alternative for the development of sustainable materials in biomedical applications. Cutin, composed primarily of fatty acids and hydroxy acids, exhibits favorable biodegradability, biocompatibility, and hydrophobic properties, positioning it as a viable candidate for applications such as drug delivery systems, wound healing, and tissue engineering. This study investigates the extraction, characterization, and potential biomedical utilization of 10,16-dihydroxy hexadecenoic acid monomer derived from tomato cutin agro-waste. The cytotoxicity of cutin-based materials was evaluated through in vitro assays, demonstrating minimal toxicity and confirming their suitability for biomedical applications. The extraction process was optimized using various solvents, and the molecular characteristics of the extracted monomer were assessed using techniques such as Gel Permeation Chromatography (GPC), Gas Chromatography–Mass Spectroscopy (GC-MS) ¹H and ¹³C Nuclear Magnetic Resonance (NMR), Fourier Transformed Infrared (FT-IR) spectroscopy, Thermogravimetric Analysis (TGA), and Differential Scanning Calorimetry (DSC). MTT assay was also performed on NHDFs cultured in monomer solutions to assess their cytocompatibility. The findings suggest that cutin-based materials, when processed under environmentally sustainable conditions, offer an effective and biocompatible alternative to conventional synthetic polymers, opening new avenues for the development of sustainable biomedical products.