Our objective was to determine the effects of feeding 25-hydroxyvitamin D₃ [25(OH)D₃], or vitamin D₃ (cholecalciferol) on plasma, mineral, and metabolite concentrations, mineral balance, mineral excretion, rumination, energy balance, and milk production of dairy cows. We hypothesized that supplementing 3 mg/d of 25(OH)D₃ during the prepartum period would be more effective than supplementing vitamin D₃ at the National Research Council (2001) levels to minimize calcium imbalance during the transition period and improve milk production of dairy cows. Forty multiparous, pregnant nonlactating-Holstein cows were enrolled in this study. Body weight, body condition score, parity, and milk yield in the previous lactation (mean ± standard deviation) were 661 ± 59.2, 3.46 ± 0.35, 1.79 ± 0.87, and 33.2 ± 6.43 kg/d, respectively. Cows were enrolled into the blocks (n = 20 for each treatment) at 30 d of the expected day of calving to receive an acidogenic diet (373 g/kg of neutral detergent fiber and 136 g/kg of crude protein, dry matter basis; -110 mEq/kg) associated with the treatments: (1) control (CTRL), vitamin D₃ at 0.625 mg/d (equivalent to 25,000 IU of vitamin D₃/d) or (2) 25(OH)D₃ at 3 mg/d (equivalent to 120,000 IU of vitamin D₃/d). All cows were fed with the base ration for 49 d after calving. Blood samples were taken on d 7, 0, 1, 2, 21, and 42, relative to calving. No effect of treatment was observed for prepartum dry matter intake or body condition score. A trend for increase of ionized Ca was observed for the cows fed 25(OH)D₃, compared with the CTRL, but no effect of treatment was detected for total Ca or total P. Feeding 25(OH)D₃ increased colostrum yield. The plasmatic concentration of 25-hydroxyvitamin D₃ was increased with 25(OH)D₃ supplementation. 25-Hydroxyvitamin D₃ supplementation increased plasma glucose concentration at parturition. The postpartum dry matter intake was not influenced by treatments. Feeding 25(OH)D₃ increases milk yield, 3.5% fat-corrected milk, and energy-corrected milk and improves milk yield components in early lactation. Overall, these findings suggest that 25(OH)D₃ at 3 mg/d can improve the energy metabolism and lactation performance, compared with the current-feeding practice of supplementing vitamin D₃ at 0.625 mg/d.

The aim of this study was to evaluate the ruminal parameters of fermentation and degradation, kinetic flow of rumen digesta, and milk fatty acid composition of cows fed 52% chopped elephantgrass-based diets containing 0.0% (control), 1.5%, 3.0% and 4.5% soybean oil (SO) on a dry matter (DM) basis. Four rumen-cannulated Holstein x Gyr dairy cows with an average milk production of 15.6 ± 3.0 kg day-1 and 90 ± 25 days in milk were allocated in a 4 x 4 Latin square design. The results were analyzed by mixed models. Significant differences were declared at P ? 0.05, and P-values from 0.05 < P ? 0.10 were considered as a trend. The inclusion of SO in the diet had no effect on the ruminal pH or total volatile fatty acid concentration, but there was a quadratic effect on the ruminal ammonia nitrogen content and a trend for a linear reduction (P = 0.07) in the molar proportion of rumen acetate. Linear reductions were also observed in the DM and neutral detergent fiber (NDF) effective degradabilities of elephantgrass forage, but the fluid and particulate passage rates in the rumen and the DM and NDF intakes were unchanged by SO inclusion in the diet. Milk production, protein and lactose contents and yields were unaltered by dietary SO levels. There were linear reductions in the milk fat and total solids contents, but there was no effect of dietary treatments on their yields. The inclusion of soybean oil in the diet of Holstein x Gyr cows fed chopped elephantgrass improved the nutritional quality of milk fat as a result of increased contents of oleic, rumenic and vaccenic acids, which are beneficial to human health, and a concomitant reduction in hypercholesterolemic saturated fatty acids such as lauric, myristic and palmitic acids.

The aim of this study was to evaluate the forage mass and accumulation and nutritional value of cultivars Kennedy and BRS Integra (Urochloa ruziziensis), subjected to two planting densities (15 and 30 plants/m²) and three stubble height (15, 30 and 40 cm). The experimental design was a randomized block, in a 2x2x3 factorial, with three replications. The experimental period comprised two agrostological years. Plants managed under lower stubble height (15cm), showed lower leaf:stem ratio and higher canopy heights at harvest, based on the light interception of 95%. The highest forage mass was observed to cultivar BRS Integra under lower planting density (15 plants/m²). The two cultivars showed high nutritive value, without influence of studied factors. In general, the cultivars were similar for most variables studied. Planting with 15 plants/m² and moderate defoliation (30cm residue) resulted in higher forage accumulation, especially in the second year after planting. These targets are suggested for the management of U. ruziziensis cultivars.

This work evaluated the productive and structural characteristics of the BRS Zuri and BRS Quênia cultivars under rotational stocking management. The evaluations were divided into five periods according to rainfall regime (high, medium, and low rainfall) during 16 months of the years 2016-2018. Pastures were grazed when canopies intercepted 95% of incident light to stubbles corresponding to 50% of the pre-grazing height. The pre- and post-grazing heights varied as a function of cultivar, with higher values for BRS Zuri [...] ABSTRACT This work evaluated the productive and structural characteristics of the BRS Zuri and BRS Quênia cultivars under rotational stocking management. The evaluations were divided into five periods according to rainfall regime (high, medium, and low rainfall) during 16 months of the years 2016-2018. Pastures were grazed when canopies intercepted 95% of incident light to stubbles corresponding to 50% of the pre-grazing height. The pre- and post-grazing heights varied as a function of cultivar, with higher values for BRS Zuri

This study aimed to evaluate the chemical composition and fatty acid (FA) profile of chopped forage and silage of BRS Capiaçu elephant grass at four regrowth ages: 50, 70, 90 and 110 days. A randomized block design with five replications was used. The ensiling was carried out manually in experimental silos without wilting using no additives or bacterial inoculants. The results were analyzed using mixed models (P < 0.05). The model included treatment (regrowth age) as a fixed effect and block as a random effect. Linear and quadratic effects of the treatments were analyzed using orthogonal contrasts. There were linear increases in the dry matter (DM, g kg-1) and lignin (g kg-1 DM) contents and linear reductions in the in vitro DM digestibility (g kg-1) of chopped grass and silage as a function of regrowth age (P < 0.001). Quadratic effects (P ≤ 0.01) were observed for the chopped grass contents (g kg-1 DM) of crude protein (CP), ether extract (EE) and neutral detergent fiber (NDF) as a function of regrowth age. There were linear decreases (P < 0.0001) in the CP content (g kg-1 DM) and pH and linear increases (P < 0.001) in the EE and NDF contents (g kg-1 DM) in the silage as a function of regrowth age. There were linear decreases (P < 0.01) in the chopped grass contents and linear increases (P < 0.05) in the silage contents of total FAs, linoleic and α-linolenic acids (g kg-1 DM) asa function of regrowth age. BRS Capiaçu elephant grass must be harvested at up to 70 days of regrowth to obtain forage with good nutritional value and the highest levels of linoleic and α-linolenic acids (g kg-1 DM). To produce silages with adequate pH values and the highest levels of linoleic and α-linolenic acids (g kg-1 DM), BRS Capiaçu must be harvested between 90 and 110 days of regrowth.