To determine the in vitro antimicrobial activity of N-acetylcysteine (NAC) against common pathogens associated with infectious keratitis in dogs and cats, clinical isolates of Staphylococcus (S.) pseudintermedius (n = 20), Streptococcus (St.) canis (n = 10) and Pseudomonas (P.) aeruginosa (n = 7) of canine and feline infectious ulcerative keratitis and a quality control strain (P. aeruginosa DSM 19880) were tested. The minimal inhibitory concentration (MIC) of NAC concentrations was determined using microdilution methodology. For S. pseudintermedius and P. aeruginosa, NAC concentrations in the range of 1.56 mg/mL (0.156%) to 100 mg/mL (10%), and for St. canis, concentrations ranging from 0.195 mg/mL (0.0195%) to 6.25 mg/mL (0.625%) were tested. For S. pseudintermedius, the MIC was 3.12 mg/mL (0.312%) for all tested isolates. For P. aeruginosa isolates and the quality control strain, the MIC ranged from 3.12 mg/mL (0.312%) to 6.25 mg/mL (0.625%). For St. canis, the MIC ranged from 1.56 mg/mL (0.156%) to 3.12 mg/mL (0.312%). NAC has an in vitro antimicrobial activity against three bacterial species commonly found in infectious keratitis in dogs and cats and therefore may be a promising alternative or adjuvant to topical antibiotics. The results warrant a clinical pilot study to assess the potential of NAC to reduce or replace the use of topical antibiotics in line with the One Health approach.

Noninvasive blood pressure (NIBP) device performance in dogs may be influenced by extreme pressures and altered systemic vascular resistance (SVR). This study evaluated the agreement of two NIBP devices (HDO and petMAP) with invasive blood pressure (IBP) measurements, compliance with hypertension consensus statement criteria, and their trending ability (TA) across varying blood pressure and SVR ranges in awake and anesthetized dogs. Seven healthy Beagles were studied, with IBP recorded from the dorsal metatarsal artery and NIBP cuffs placed randomly on the front limb, hind limb, or base of the tail. Cardiac output was determined by thermodilution, and the systemic vascular resistance index (SVRI) was calculated by a standard formula. Bland–Altman, concordance rate, and polar plot analyses were used for statistical analysis. A total of 752 and 640 paired measurements were obtained for HDO and petMAP, respectively. Both devices showed good agreement with IBP for mean arterial pressure (MAP) at low blood pressure and the SVRI. At high blood pressure and the SVRI, agreement weakened, with substantial underestimation of systolic arterial pressure (SAP). Both devices demonstrated moderate to good TA for MAP and SAP. Overall, the best agreement was observed for MAP at a low SVRI, while agreement was moderate at hypertension (petMAP) and a high SVRI (petMAP, HDO).