Undisturbed soils are essential ecosystems with high microbial diversity. Microorganisms present in the soil can regulate biogeochemical cycles, making available and transforming different minerals in the soil, such as nitrogen, phosphorus and sulfur. In this study, the microbiota of undisturbed soils was characterized using an integrated approach of 16S rRNA ribosomal gene amplicon analysis and classical microbiology techniques. Phylum-level analyses revealed a high abundance of Proteobacteria, Acidobacteria, Verrucomicrobia and Actinobacteria, key groups in nutrient recycling, organic matter decomposition and plant-microorganism interaction. In the genus analysis, Nitrospira spp., Candidatus Koribacter spp., Burkholderia spp., Bacillus spp., Flavobacterium spp. and Pedomicrobium spp. were identified, with important functions in nitrification, plant growth promotion, organic matter degradation, and recovery of degraded soils. On the other hand, by using selective and differential media, it was possible to demonstrate the presence of microorganisms such as Enterobacter spp. and Hafnia spp., with the ability to solubilize phosphorus and potassium and produce siderophores, which are likely contributing to the biogeochemical cycles and plant growth within the soil studied.