Miombo is the most extensive ecosystem in southern Africa, being strongly driven by fire, climate, herbivory, and human activity. Soils are major regulating and supporting services, sequestering nearly 50% of the overall carbon and comprising a set of yet unexploited functions. In this study, we used next-generation Illumina sequencing to assess the patterns of bacterial soil diversity in two pioneer Miombo species, Brachystegia boehmii and Brachystegia spiciformis, along a fire gradient, in ferric lixisol and cambic arenosol soils. In total, 21 phyla, 51 classes, 98 orders, 193 families, and 520 genera were found, revealing a considerably high and multifunctional diversity with a strong potential for the production of bioactive compounds and nutrient mobilization. Four abundant genera characterized the core microbiome among plant species, type of soils, or fire regime: Streptomyces, Gaiella, Chthoniobacter, and Bacillus. Nevertheless, bacterial networks revealed a higher potential for mutualistic interactions and transmission of chemical signals among phylotypes from low fire frequency sites than those from high fire frequency sites. Ecological networks also revealed the negative effects of frequent fires on the complexity of microbial communities. Functional predictions revealed the core “house-keeping” metabolisms contributing to the high bacterial diversity found, suggesting its importance to the functionality of this ecosystem.

The wild dioecious plant Corema album, endemic to the Atlantic coast of the Iberian Peninsula, has potential to become a new crop and to integrate the berry fruit market. Due to its potential, the aim of this study was to propose a BBCH (Biologische Bundesanstalt, Bundessortenamt and CHemical Industry) scale for the species. Based on field observation, the principal growth stages were described and sequentially established. Photographs and stereomicroscope acquisition software were used to illustrate the phenological stages of male and female plants. The scale includes seven principal stages starting with bud development (stage 0), followed by leaf development (Stage 1), main stem elongation (Stage 3), inflorescence emergence (Stage 5), flowering (Stage 6), fruit development (Stage 7) and ending with fruit maturation (stage 8), with mesostages for male and female inflorescence (stage 5) and flower development (stage 6). Through field observations carried out in plants in different growth conditions, the proposition of this extended BBCH scale was made, to have a standardized system for Corema album phenological stages. This study will have an application especially in fields of agronomy and plant breeding and will also be a stepping stone for the inclusion of this species as a novelty crop in a nearby future.