This study aimed to evaluate an auto-verification system for serological markers of infectious diseases in a clinical laboratory, with the goal of ensuring reliable results while shortening the turnaround time for reports, reducing the need for human resources, and improving work efficiency. Based on the guidelines and clinical requirements, personalized auto-verification rules were established. Then, the serological marker results of 144,927 samples (including hepatitis B, hepatitis C, Treponema pallidum, and human immunodeficiency virus) were used for verification, and the performance of the auto-verification system was evaluated. The auto-verification system enabled our laboratory to shorten the turnaround time by 21.13% (from 369.54 minutes to 291.47 minutes) while increasing the sample volume. The pass rate, sensitivity, specificity, false positive rate, and false negative rate of the auto-verification system were 54.81%, 100%, 54.99%, 45.01% and 0%, respectively. Further analysis of the reasons for the failure of auto-verification revealed that a portion of the samples were blocked due to technical issues with the laboratory information system. Further improvements are needed in the future. Establishing an appropriate auto-verification system based on the actual situation of the laboratory is an important means to ensure medical safety, while improving the efficiency of the clinical laboratory and reducing labor costs.

The endoplasmic reticulum–Golgi apparatus system is an important organelle regulating male reproduction. USO1 vesicle transport factor (USO1), as an important molecule in this system, is a general vesicular transport factor and regulates various biological processes in vivo. However, the potential role of USO1 in mammalian testis development and spermatogenesis has not been investigated. We documented the presence of USO1 in mouse germ cells and its functional roles by generating Uso1-knockout germ cell lines. Uso1 depletion suppressed cell proliferation and growth while stimulating apoptosis in GC1 and GC2 cells. In addition, the Uso1 knockout blocked cell cycle progression and weakened DNA damage repair. Mechanistically, USO1 is associated with male reproduction by regulating the expression of genes involved in spermatogenesis, and we found evidence that USO1 is closely linked to centriolar satellites (CSs), which may play an important biological role. Overall, our findings reveal a vital role for USO1 in male fertility and offer a significant understanding of the functions of golgin proteins in reproductive biology.