Background: Antibiotics are widely used in food animals to prevent disease, promote growth and maintain productivity. Modern animal production practices involve regular use of antimicrobials, but abusive use can lead to harmful consequences. The aim of this review was to provide comprehensive information regarding the risk of regular application of antibiotics in food animals, the potential consequences and impact on human health, and options for the prevention of antibiotic resistance in the food chain. Review Results: Although organizations such as WHO, UN, and EU have taken steps to reduce and limit the use of antibiotics in animals, these are proving insufficient in developing countries. In many countries, the antibiotics used in animals appear to exceed that in humans. The abuse in food animals promotes the development of antibiotic-resistant bacteria and resistance genes that can be transmitted to humans through food consumption and direct contact with animal food or environment mechanisms. This can lead to human infections with antibiotic-resistant bacteria, causing treatment failure and, therefore, more severe and longer-lasting illnesses, increased hospitalization rates, more deaths and higher costs to society. Furthermore, as food animals and animal-derived foods are traded globally, they contribute to antibiotic resistance in countries far from where the problem originates. Conclusion: Overcoming the problem of antibiotic resistance requires a multidisciplinary approach with effective action coordination and information exchange between the agricultural, food, veterinary and health sectors. Through international cooperation, efforts should focus on reducing unnecessary antibiotics use and reducing the spread of antibiotic-resistant bacteria, but national-level action on antibiotic resistance and food safety is also essential.

Respiratory syncytial virus (RSV) and rhinovirus (RV)—the two primary causative viruses of bronchiolitis in children—have been studied extensively in terms of their associations with disease severity and serious late disease outcomes. In this study, we explored the differences in the clinical values of IFN-γ and IL-10 serum levels in RSV and RV bronchiolitis in the Bulgarian childhood population. Eighty-eight children with acute bronchiolitis, aged two months to two years, who were admitted to the General Pediatrics Clinic of University Hospital “Prof. St. Kirkovich”, Stara Zagora, Bulgaria served as this study’s subjects. The degree of wheezing and respiratory failure were classified. Naso-pharyngeal swabs were collected from all participants, and molecular identification of viruses was performed using real-time PCR. Serum samples were used to determine IFN-γ and IL-10 quantities using ELISA kits, and data are presented as the median IQR (25–75%). The total serum IL-10 levels were significantly enhanced in RSV-infected children compared to those infected with RV (14.4 (12.2–24.0) vs. 8.9 (7.2–12.5); p < 0.001) and the other viral bronchiolitis groups (14.4 (12.2–24.0) vs. 6.65 (3.9–15.3); p = 0.003). The highest IL-10 levels (14.4 (12.8–27.9)) were found in RSV-positive patients with first-degree respiratory failure. Almost identical serum IFN-γ levels were determined for RSV- and RV-positive bronchiolitis patients (3.2 (1.6–6.8) and 2.8 (1.1–7.3); p = 0.781). Variance analysis of IL-10 serum levels revealed statistically significant differences among the patient groups depending on the type of viral infection, concerning respiratory failure (p = 0.005) and wheezing severity (p = 0.017). Our findings show that the IL-10 levels and the type of virus have a combined effect on disease severity. These data might contribute to patients’ personalized/individualized therapy and the prevention of recurrent wheezing later in life.

Background: Food safety is of utmost importance to sustaining life and promoting good health and is a main problem in any country, regardless of economic and social development. Unsafe food containing harmful pathogens and their toxins cause hundreds of diseases, particularly affecting infants, young children, the elderly and sick people. Therefore, the rapid detection of foodborne pathogens is of great significance for public health. The aim of this review was to provide comprehensive information regarding methods for the detection of foodborne pathogens as well as genus Fusarium and their toxins, from fundamental to state-of-the-art, presenting their advantages and limitations in order to update current knowledge. Review Results: The traditional culture-based methods for the detection of pathogens in food are usually laborious, limited by complex sample preparation procedures, time-consuming, slow to provide results, and require well-trained professional staff. However, compared to these methods, the new ones - immunological methods, nucleic acid-based assays and biosensor-based methods are rapid, accurate, highly sensitive and specific, and easy to use. Precisely providing accurate real-time results would help limit foodborne disease outbreaks, ensure compliance with legislation setting maximum levels of pathogens in certain food categories, detect and improve the import of food contaminated with dangerous levels of pathogenic microorganisms, and ensure public health safety. Conclusion: The development of rapid and automated methods that detect real-time, small numbers of viable microbial cells within a given volume of food is vital in the prevention, transmission and treatment of foodborne diseases. Such methods would also offer a great commercial advantage in the food industry and related fields.

Fusarium spp. represent a critical threat to maize production and food safety due to their mycotoxin production. This study introduces a refined molecular identification protocol integrating four genomic regions—ITS1, IGS, TEF-1α, and β-TUB—for robust species differentiation of Fusarium spp. isolates from post-harvest maize in Bulgaria. The protocol enhances species resolution, especially for closely related taxa within the Fusarium fujikuroi species complex (FFSC). A newly optimized multiplex PCR strategy was developed using three primer sets, each designed to co-amplify a specific pair of toxigenic genes: fum6/fum8, tri5/tri6, and tri5/zea2. Although all five genes were analyzed, they were detected through separate two-target reactions, not in a single multiplex tube. Among 17 identified isolates, F. proliferatum (52.9%) dominated, followed by F. verticillioides, F. oxysporum, F. fujikuroi, and F. subglutinans. All isolates harbored at least one toxin biosynthesis gene, with 18% co-harboring genes for both fumonisins and zearalenone. This dual-protocol approach enhances diagnostic precision and supports targeted mycotoxin risk management strategies.

Background: Enterococci are ubiquitous and can be found in water, soil, plants and several food products. They are also considered part of the normal beneficial gastrointestinal flora of both humans and animals. The aim of this review was to provide comprehensive information regarding the benefits and risks of Enterococcus speciesuse in food or as probiotics. Review Results: The presence of enterococci in foodstuffs has long been considered a sign of poor sanitation. Contrariwise, due to their enzymatic and proteolytic activities, they are used as starters in food fermentation and play an important role in the development of the organoleptic properties of fermented foods. Enterococci can spoil processed meats but, in contrast, are important for the maturation and flavor development of some traditional cheeses and sausages. Because of the ability to produce bacteriocins possessing antimicrobial action, enterococci are used as protective cultures in food biopreservation. Additionally, they showed probiotic properties with various beneficial characteristics. However, in the last few decades, enterococci have emerged as one of the most important bacteria worldwide, major opportunistic agents causing a wide range of nosocomial infections. They are resistant or tolerant to many antimicrobials, and multidrug resistant enterococci belong to the so-called "therapeutic problematic microorganisms". According to WHO, vancomycin-resistant representatives are high-priority pathogens on the list of microorganisms for the treatment of which the development of new antibiotics is urgently needed. In addition, they possess multiple virulence factors. Conclusion: The role of enterococci in the pathogenesis of numerous and severe infections requires serious comments and discussion about the benefits and risks regarding their presence in food and their use as probiotics.