This study assessed axial and appendicular fractures in crab-eating foxes (Cerdocyon thous; Linnaeus, 1766) admitted to the Reference Center for Wildlife. Medical records and plain radiographs of C. thous were retrospectively evaluated. Seventy-four free-ranging C. thous were admitted over 10 years, of which 21 had fractures in the appendicular and/or axial skeleton. Appendicular skeleton fracture was verified in 42.86 % of the animals, five of whom had long bone involvement and four the hip bones. More than one fracture in the appendicular skeleton occurred in 14.28 % of the animals, with the most common being a long bone fracture with a hip fracture. Axial skeleton fractures were observed in 9.52 % of the cases, with one fracture located in the vertebral column and the other in the head. Five animals died and nine were euthanized due to injury severity, prognosis and/or poor outcome after treatment. Seven animals showed good evolution after treatment, and four animals were released. In conclusion, hip bone fractures, isolated or combined with injuries to other bones of the axial and/or appendicular skeleton, were the most common injuries in C. thous. The characteristics of multiple trauma were associated with high mortality and complications.

In vitro embryo production (IVP) protocols generally use supplementation with fetal bovine serum (FBS) in its culture media. Although it contains essential nutrients and growth factors for the embryo, exposure to serum can affect embryonic morphology and biochemistry, in addition to being possibly related to increased fetal weight at birth. In this sense, multipotent stromal cells (MSCs), given the wide range of secreted factors, could help improve IVP protocols. This study verified whether cell proliferation factors secreted by multipotent stromal cells obtained from endometrial tissue (eMSCs) stimulate the development and production of better quality embryos. For that purpose, bovine embryos produced in vitro were co-cultured with eMSCs and evaluated for cleavage index and blastocyst formation, as well as total cell count, trophoblast cell count and apoptotic cell count. It is concluded that the production of bovine embryos associated with co-culture with eMSCs presented similar results to the use of traditional media, showing an improvement regarding the total number of cells, in which the embryos cultivated in the presence of eMSCs and absence of FBS presented a higher number of total cells than those cultured in the absence of eMSCs and FBS.

A retrospective analysis was performed on free-ranging crab-eating foxes (Cerdocyon thous) admitted to a Center for Wildlife Medicine and Research (CEMPAS), located in Botucatu, during a 9-year period (2013-2021). A total of 73 animals were evaluated, comprising 41 adults, 14 subadults, and 18 pups. Regarding sex, 45 were males, 23 were females, and 5 the information was not provided. The causes of admission included rescued healthy animal (n = 25), animals with fractures due to motor vehicle collision (n = 13), animals with fractures of unknown cause (n = 10), traumatized animals with no fracture (n = 11), and other health conditions (n = 14). The lowest number of admissions was observed in 2019 and 2020. Included all years, the spring showed the highest number of admitted animals. Among the healthy animals, 44% (11/25) remained in the institution, 24% (6/25) died, 16% (4/25) were released back into the wild, 4% (1/25) were submitted to euthanasia, 4% (1/25) escaped from the enclosure and 8% (2/25) had no outcomes information. Regarding individuals with fractures due to motor vehicle collisions, 46.1% (6/13) were submitted to euthanasia, and 30.8% (4/13) were released back to nature, and 23.1% (3/13) died. Of those rescued due to fractures of unknown cause, 50% (5/10) were submitted to euthanasia, 30% (3/10) died, 10% (1/10) remained in the institution, and 10% (1/10) were released back to the nature. The geographic distribution of Cerdocyon thous rescues admitted to CEMPAS between 2013 and 2021 was concentrated in Botucatu and surrounding municipalities, likely reflecting the institution’s area of operation. This scenario represents a significant challenge for species conservation and requires the adoption of complementary measures to mitigate its negative impacts. Among these, the implementation of public policies aimed at reducing wildlife–vehicle collisions and preventing interactions between domestic animals and wildlife stands out as essential. In summary, most Cerdocyon thous individuals admitted to the CEMPAS were adult males, rescued near paved roads and frequently presenting signs of trauma. These findings highlight the crucial role of Wildlife Rescue and Rehabilitation Centers in mitigating anthropogenic threats to wildlife.

An adult female southern tamandua (Tamandua tetradactyla) was rescued and taken to a veterinary centre for wildlife care. The patient's evaluation revealed an abnormal neurological status, along with prostration, diarrhoea, dehydration and bilateral corneal ulcers. Insecticide poisoning (carbamate, organophosphate or organochlorine) and canine distemper were initially considered as possible diagnoses, and supportive treatment was instituted. During screening with abdominal ultrasound and radiographic examinations, an advanced pregnancy stage was identified. Four days after its admission, the female experienced profuse oral and vaginal bleeding, abdominal contractions and fetal heart rate oscillations. The team decided to wait for natural delivery or abortion, considering the female's vulnerable clinical condition. Fetal death was confirmed by ultrasound within the following 12 hours, leading to an emergency caesarean section. The surgical approach was successful, and the patient showed progressive clinical improvement, returning to the natural environment after a month of recovery.