Tobacco cultivation and industrialization are characterized by the production of trillions of pre-harvest and post-harvest waste biomasses each year with the resulting negative effects on the environment. The leaves of blunt, pre-harvest waste, could be further used to obtain bioactive metabolites, i.e., polyphenols and alkaloids, for its potential cosmetic use. This study was conducted to obtain bio-compounds from pre-harvest tobacco leaf waste (var. Virginia) by applying conventional and green solvents (NaDES). Leaves and ground leaf waste were characterized based on their microscopic features. Conventional solvents, such as water, acetone, ethanol, and non-conventional solvents, such as Natural Deep Eutectic Solvents (NaDES), i.e., sucrose:lactic acid (LAS), frutose:glucose:sucrose (FGS), lactic acid:sucrose:water (SALA), choline chloride:urea (CU), and citric acid: propylene glycol (CAP) were used for bioactive extraction from tobacco waste powder. CU, FGS, and acetone/ethanol had similar behavior for the best extraction of alkaloids (6.37–11.23 mg ACE/g tobacco powder). LAS, FGS, SALA, and CU were more effective in phenolic compound extraction than conventional solvents (18.13–21.98 mg AGE/g tobacco powder). Because of this, LAS and SALA could be used to obtain phenolic-enriched extracts with lower alkaloid content rather than CU and FGS. Extracts of the powder obtained with conventional solvent or CU showed a high level of sugars (47 mg/g tobacco powder) The ABTS antioxidant capacity of tobacco leaf powder was higher in the extracts obtained with CU, FGS, and acetone (SC₅₀ 1.6–5 µg GAE/mL) while H₂O₂ scavenging activity was better in the extracts obtained with LAS, CAP and SALA (SC₅₀ 3.8–8.7 µg GAE/mL). Due to the biocompatibility of the NaDES with the components of tobacco leaf waste, the opportunity to apply these extracts directly in antioxidant formulations, such as cosmetics, phytotherapic, and other formulations of topic use seems promising. Furthermore, NaDES constituents, i.e., urea and organic acid can also have beneficial effects on the skin.

Some Plant Growth-Promoting Rhizobacteria (PGPR) can induce protection against pathogens, increasing plant tolerance to various diseases. This so-called biocontrol activity is replacing harmful practices in agriculture caused by the use of agrochemicals. Azospirillum brasilense is one of the PGPR already effectively used as a resistance inducer in several crops. The aim of this study was to evaluate the protective effect of PGPR A. brasilense strains isolated from strawberry and petunia plants (REC3, 2A1, 2A2, and 2E1) against the fungal pathogen Macrophomina phaseolina, which is the causal agent of the strawberry charcoal rot disease. In vitro antagonism assays and enzymatic tests on Petri dishes revealed no direct inhibition on M. phaseolina growth by any of the A. brasilense strains. However, strawberry plants treated with REC3 and 2A1 strains increased callose and lignin deposition and stomatal closure compared to untreated plants. In addition, treatments with either bacterial strains induced a defense response in strawberry plants against virulent isolates of M. phaseolina evidenced by an increased tolerance to the charcoal rot disease. These results suggest that A. brasilense REC3 and 2A1 strains can be used for the activation of innate immunity in strawberry plants as a strategy for managing charcoal rot in a sustainable and environmentally friendly way.

The production of smokeable tobacco for use in cigarettes is characterized by the production of pre-harvest and post-harvest waste, with ensuing undesirable effects on the environment. The inflorescences of tobacco after blunting, deflowering, or topping are considered pre-harvest waste and left in the field. Using green and ecofriendly solvents such as Natural deep eutectic solvents (NaDESs), these wastes could be used to obtain antioxidant molecules of interest in cosmetics. Taking into account its potential as plant matrix to obtain metabolites of commercial interest, tobacco inflorescences and inflorescence powders of different particle sizes were characterized by optic and electronic microscopy. Thus, the powdered inflorescences were extracted with four conventional solvents, i.e., distilled water (DW), acetone: distilled water (AW), ethanol 70° (EW), methanol (Me), and five NaDESs, i.e., lactic acid: sucrose (LAS), lactic acid: sucrose: distilled water (SALA), fructose: glucose: sucrose: distilled water (FGS), choline chloride: urea: distilled water (CU), and citric acid: propylene glycol (CAP). Among the tested NADESs, SALA was the most promising solvent; higher extraction yields of total phenolic compound (3420.0 ± 9.4 µg GAE/mL) than conventional solvents were attained and it was the only selective solvent to phenolics. CU was the best solvent for flavonoids and alkaloids extraction (215.3 ± 3.2 µg QE/mL and 392.3 ± 8.0 µg ACE/mL, respectively). All extracts showed antioxidant activity. A heatmap with dendrogram and main component analysis showed that acid-based NaDESs are grouped together, this group being the one with the best performance in H₂O₂ scavenging. The extracts obtained with green solvents could be used directly in cosmetic formulations as antioxidant ingredients because both tobacco flower oil and flower extracts are listed in the cosmetic ingredients database as non-toxic products. Additionally, the demand for sustainable ecological cosmetics is growing. In this sense, NaDESs represent an opportunity to develop innovative extracts with unique phytochemical fingerprints and biological activities.

Arbuscular mycorrhizal fungi (AMF) are symbiotic fungi that associate with the vast majority of terrestrial plants. Among non-vascular plants, while AMF associations are well-documented in liverworts and hornworts, there is a broad consensus that symbiotic associations do not occur in mosses. Here, we report the presence of AMF in the living material of mosses found in Chaco Serrano (Tucumán, Argentina). We found all characteristic structures of AMF when establishing an intimate connection with two moss species of Pottiaceae (Bryophyta). While Gertrudiella uncinicoma exhibited AMF with both Arum- and Paris-type morphologies, Pleurochaete luteola only displayed an Arum-type morphology. Plant tissue samples were subjected to high-throughput sequencing for AMF identification. We determined that Rhizophagus irregularis was a clear dominant species in both moss species, with Glomus sp. also being present as a less abundant element. In addition, we also reported the presence of vesicles, arbuscules, and spores adhered to the hyphae and the presence of septate endophytes. This finding expands our understanding of the interactions between AMF and non-vascular plants and prompt us to further characterize this interaction by considering the diversity of mycorrhizal associations with concurrent implications for the ecology of mosses and the functionality of the ecosystems.

Adesmia trijuga es un arbusto espinoso, micrófilo, nativo, perenne; crece entre 1100-3000 m snm, en regiones montañosas de quebradas húmedas o zonas áridas y rocosas; vulgarmente es conocida como “cuerno de cabra”, “añagua” o “suncho”. A. trijuga es polimórfica de hojas pinnadas con 3-5 pares de folíolos, forrajera, utilizada como leña y de amplia distribución en Argentina y Chile. Los antecedentes se refieren únicamente a la citogenética de poblaciones de la Región de Cuyo. El objetivo fue caracterizar los parámetros micrográficos vegetativos, histoquímica y citogenética de A. trijuga en poblaciones del NOA. Se recolectaron, al azar, 5 individuos en poblaciones ubicadas en Jujuy, Salta y Tucumán. El material fue fijado en FAA y herborizado; se aplicaron técnicas anatómicas, histoquímicas y citogenéticas convencionales. Los resultados evidenciaron hojas paripinadas, micrófilas, con folíolos de lámina entera, simétrica, obovada y venación pinnada-camptódroma-eucamptódroma. Foliolos anfistomáticos, pubescentes en ambas epidermis, con tricomas eglandulares y glandulares de 3 tipos; células epidérmicas isodiamétricas a rectangulares de paredes rectas a curvas con cutícula estriada; estomas anomocíticos, hemibraquiparacítico, braquiparacítico, anfibraquiparacítico, hemianfibraquiparacítico y hemianficiclocítico; mesofilo equilateral; haces vasculares colaterales con casquete de esclerénquina hacia floema y vaina parenquimática. Pecíolo subcircular, con tricomas eglandulares y glandulares idénticos a los de la lámina; 2-3 estratos de clorénquima en empalizada subepidermico; 5 haces vasculares colaterales. Espinas circulares con tricomas idénticos a los de la lámina y el pecíolo, crecimiento secundario con abundante esclerénquima. Tubos e idioblastos con fenoles se presentan en el mesofilo de la hoja, médula del pecíolo y en la espina. El número cromosómico fue de 2n=20 cromosomas. Los elementos de valor diagnóstico son: tipo de tricomas, cantidad y posición del tejido de sostén, idioblastos y tubos productores de fenoles y el número cromosómico.