The present work reports a simple, cost-effective, and ecofriendly method for the synthesis of silver nanoparticles (AgNPs) using Chrysanthemum indicum and its antibacterial and cytotoxic effects. The formation of AgNPs was confirmed by color change, and it was further characterized by ultraviolet–visible spectroscopy (435 nm). The phytochemical screening of C. indicum revealed the presence of flavonoids, terpenoids, and glycosides, suggesting that these compounds act as reducing and stabilizing agents. The crystalline nature of the synthesized particles was confirmed by X-ray diffraction, as they exhibited face-centered cubic symmetry. The size and morphology of the particles were characterized by transmission electron microscopy, which showed spherical shapes and sizes that ranged between 37.71–71.99 nm. Energy-dispersive X-ray spectroscopy documented the presence of silver. The antimicrobial effect of the synthesized AgNPs revealed a significant effect against the bacteria Klebsiella pneumonia, Escherichia coli, and Pseudomonas aeruginosa. Additionally, cytotoxic assays showed no toxicity of AgNPs toward 3T3 mouse embryo fibroblast cells (25 µg/mL); hence, these particles were safe to use.

Green synthesis of nanoparticles can be an important alternative compared to conventional physio-chemical synthesis. We utilized Scadoxus multiflorus leaf powder aqueous extract as a capping and stabilizing agent for the synthesis of pure zinc oxide nanoparticles (ZnO NPs). Further, the synthesized ZnO NPs were subjected to various characterization techniques. Transmission electron microscope (TEM) analysis showed an irregular spherical shape, with an average particle size of 31 ± 2 nm. Furthermore, the synthesized ZnO NPs were tested against Aedes aegypti larvae and eggs, giving significant LC₅₀ value of 34.04 ppm. Ovicidal activity resulted in a higher percentage mortality rate of 96.4 ± 0.24 at 120 ppm with LC₅₀ value of 32.73 ppm. Anti-fungal studies were also conducted for ZnO NPs against Aspergillus niger and Aspergillus flavus, which demonstrated a higher inhibition rate for Aspergillus flavus compared to Aspergillus niger.