Reducing the punicalagin content is an effective strategy for eliminating the astringency of pomegranate juice. In this study, pomegranate juice was used as the raw material, and tannase was applied to convert punicalagin into ellagic acid and gallic acid. The effects of tannase concentration, reaction time, and temperature on juice deastringency were evaluated, along with the antioxidant and physicochemical properties of the treated juice. The results demonstrated that, under optimal conditions (33.9 U/100 mL tannase, 30 °C, 90 min reaction time), the punicalagin content decreased by 27.8%, while the ellagic acid and gallic acid levels increased by 24.2% and 32.3%, respectively, effectively reducing the juice’s astringency. Under these conditions, the total phenolic content reached 110 mg/100 g, with a free radical scavenging capacity of 69.8%, significantly enhancing the juice’s antioxidant properties. These results suggest that tannase treatment of pomegranate juice enhances the polyphenol content, thereby improving its health benefits without compromising the product quality.
This study develops a novel dual-layer chitosan (CS)/pectin film incorporating grape skin anthocyanin extract (GSAE) and lignin to address critical limitations in cherry preservation. Unlike traditional methods that leave harmful residues, this bilayer design separately integrates functional components: GSAE for targeted antioxidant/antibacterial action and lignin for ultraviolet (UV) blocking. This targeted incorporation enables synergistic performance unattainable with single-layer or conventional approaches. The films, fabricated with lignin concentrations from 1% to 15% (w/v), demonstrated excellent mechanical integrity (assessed with structural characterization), optimized gas barrier performance, and effective UV attenuation (achieved via lignin incorporation). Antibacterial analyses confirmed substantial inhibition against Staphylococcus aureus and Escherichia coli. Crucially, cherry preservation tests showed that the 15% lignin film (PG/CL15%) reduced weight loss, preserved firmness, and extended shelf life by 8 days—a significant quantitative improvement over uncoated fruit. Structural characterization (TGA, FT-IR, and XRD) verified successful GSAE/lignin embedding via hydrogen bonding. Beyond cherries, this dual-layer, bio-based design offers a promising template for the active packaging of other perishable produce sensitive to oxidation, microbial spoilage, and UV degradation, which enhances its industrial relevance.
Daqu quality plays a crucial role in the entire fermentation process of Baijiu. There is no empirical evidence for a scientific consensus on the storage period of Jiang-flavor Daqu and its quality evaluation. This study took Jiang-flavor Daqu from a liquor enterprise in Sichuan Province as the research object. It explored the changes in physicochemical indexes, microbial communities, and volatile flavor substances of the Daqu within 0–180 days of storage. Combined with simulated brewing experiments, it analyzed the effects of different storage periods of Daqu on fermented grain fermentation and the base wine quality and clarified the metabolic differences between Daqu stored for 30 days and 180 days by means of metabolomics. The results showed that the saccharification power and fermentation power of Daqu first increased and then stabilized, reaching 205 mg/g·h and 0.71 g/g·72, respectively, at 180 days. The microbial diversity first increased and then decreased, with Virgibacillus and Oceanobacillus alternately serving as the dominant bacteria. The flavor substances were more abundant within 60 days of storage, while the content of pyrazine compounds was the highest at 180 days. The wine yield of Daqu stored for 30 days was 2.26 times that of Daqu stored for 180 days. The brewing stage had the greatest impact on metabolites, and flavonoid synthesis was the key metabolic pathway. This study provides theoretical support for the scientific storage of Jiang-flavor Daqu and the standardization of its quality.