This study investigates the rare seaweed alga Dictyota bartayresiana lamour for biological activity. Antioxidant and antibacterial activities were examined. An MTT assay was carried out to examine cytotoxicity activity against colon cancer cells. The HPTLC analysis was performed for four different extracts, which exhibited clear flavonoid band formation at 254 nm and 366 nm with varied ranges of R_f values: methanolic extract (R_f 0.87), acetone extract (R_f 0.82), and benzene (R_f 0.83). Methanolic Extract Fraction One (MEF1) has a distinct band formation at 366 nm, it is shown to have the highest inhibition (6.20 ± 0.53 mm) against Escherichia coli, and the MTT assay reveals that the aqueous extract of Dictyota bartayresiana extract has an IC₅₀ value of 300 µg/mL. It is divulged that methanolic extract shows the highest phytochemical compound level among the four extracts of Dictyota bartayresiana. A GC/MS analysis was employed to investigate the flavonoid profile of the crude seaweed extract. Although LC/MS is typically preferred for flavonoid analysis due to thermal sensitivity, GC/MS was used in this study owing to time constraints, with optimized conditions to reduce thermal degradation. The GC-MS analysis identified Quinoline and other flavonoids, suggesting potential bioactivity. The cytotoxicity activity of MEF1 shows that the development of a promising drug may be evaluated from a seaweed source. The present study provides excellent insight with the first report of the biologically active compound of Dictyota bartayresiana.

The main objective of this was to evaluate the potential drug for treating colon cancer and compound must be extracted from natural source especially in seaweed. The above mentioned compounds taken from GC-MS studies of Dictyotabartayresiana. There are more number of compounds present in GC-MS analysis but hexadecane, hexadecanoic acid methyl ester and quinoline, 1,2-dihydro-2,2,4-trimethyl compounds have shown best acivities compared to others that proven in previous research. NIST and SDBS databases are helps to draw the compound structure, name, fragments, molecular weight, chemical formula, hydrogen bond donor and acceptor count. The protein were obtained from PDB (Research Collaboratory for Structural Bioinformatics, Protein Data Bank) which is repository for 3D structural data of large biological macromolecules. Auto Dock tools was utilized to generate grids, calculate dock score and evaluate the conformers of activators bound in the active site of protein as targets. The compound hexadecanoic acid methyl ester (C17 H34 O2) has the binding affinity of -6.60 kcal/mol that similar to results obtained from Quinoline, 1,2-dihydro-2,2,4-trimethyl- (C12 H15N) and Hexadecane (C16H34) shows the binding affinity of -6.20 kcal/mol which greater than standard drug 5-fluorouracil (C4H3FN2O2). In present study technically proven the anticancer property of Dictyotabartayresiana. To conclude, these resulting compounds are might be an alternate to synthetic anticancer drugs available in the market.