The metabolic system and immunology used to be seen as distinct fields of study. Recent developments in our understanding of how the immune system operates in health and disease have connected these fields to complex systems. An effective technique for identifying probable abnormalities of metabolic homeostasis brought on by disease is metabolomics, which is defined as the thorough study of small molecule metabolic intermediates within a biological system that collectively make up the metabolome. A prognostic metabolic biomarker with adequate prognostic accuracy for tuberculosis progression has recently been created. The rate-limiting host enzyme for the conversion of tryptophan to kynurenine, indoleamine 2,3-dioxygenase (IDO), is greatly elevated in the lungs of tuberculosis disease patients. Targeted study on tryptophan in tuberculosis disease indicates that such decreases may also resembled this upregulation. Although tuberculosis diagnosis has improved with the use of interferon release assay and tuberculosis nucleic acid amplification, tuberculosis control is made difficult by the lack of a biomarker to diagnose active tuberculosis disease. We hope that the reader of this work can develop an understanding of the advantages of metabolomics testing, particularly as a sort of testing that can be used for both diagnosing and monitoring a patient’s response to treatment for tuberculosis.

Approximately 10–35% of people living with HIV (PLHIV) on antiretroviral therapy (ART) fail to restore CD4⁺ T cell counts, a state known as immunological non-responder (INR) characterized by persistent immune activation and elevated cytokine levels. Objective: This study aimed to identify cytokines that can serve as biomarkers for immune activation and inflammation in INR patients. Methods: We conducted a cross-sectional study comparing two groups: INRs (PLHIV on ART with viral suppression) and immunological responders (IRs). We analyzed 40 samples of virologically suppressed PLHIV, measuring CD4⁺ T cell counts, viral load via RT-PCR, and cytokine levels through cytometric bead array (CBA). Results: The INR group exhibited significantly higher median serum levels of IL-6 (1.74 pg/mL vs. 0.94 pg/mL, p = 0.016) and IL-10 (1.65 pg/mL vs. 0.92 pg/mL, p = 0.03) compared to the IR group. Conclusions: Elevated IL-6 and IL-10 levels may serve as potential markers to distinguish INR from IR patients with areas under the curve (AUC) of 0.731 and 0.707, respectively.

Rainfall data in West Sumatra in 2001–2018 is assumed to have a seasonal pattern. Several methods can be used to model this data, including SSA and SARIMA. Each method has a different approach but has its own advantages. Therefore, a comparison will be made between the two based on the MAPE. According to the forecasting result, obtained MAPE for SSA is 17% and 22.75% for SARIMA.

Tetralogy of Fallot (TOF) is a congenital heart disease that is caused by four heart defects, namely VSD, overriding aorta, obstruction of the right ventricular outflow tract and right ventricle hypertrophy. These abnormalities, which affect the anatomic structure of the heart, cause poor oxygenated blood to be pumped out of the heart. Children have smaller veins than adults, which make obtaining blood sample much harder. An experimental design was utilized, which allows the researchers to manipulate one or more variables and measure the resultant effects on the dependent variables. The population in this study was all vein blood sample of paediatric patients under the age of ten in National Cardiovascular Centre Harapan Kita Jakarta with TOF. Eleven sample were choose randomly as the sample in this study. We took 3 millilitres of blood from each sample and gave Li-heparin. The samples were then stored for 30 minutes at 5° C, 22-25° C and 37 ° C. We then used these sample to conduct ALT and AST tests. Based on the results of these tests we conducted descriptive statistical analysis, the normality test, homogeneity test and ANOVA test. We found that there is no significant difference in the results of AST and ALT tests using the three different samples. This study aimed to examine the effects of different temperatures on the volume of Li-heparin plasma and transaminase activity in human blood with Tetralogy of Fallot. The highest plasma volume was obtained from the sample that was put 30 minutes at 37°C.