Soil mulching is one of the common measures applied in organic agricultural production which could replace plastic films and protect the environment. In order to fully evaluate the effects of different straw mulching thicknesses on soil health, maize straw was mulched with the thicknesses of 0 cm (CK), 2 cm, 4 cm and 6 cm on soil surface to assess the effects on soil temperature (ST) and moisture (SM), soil pH, soil organic carbon (SOC), total nitrogen (TN), C/N, soil aggregates and soil bulk density (SBD) in a temperate organic vineyard. We found that straw mulching had a significant regulating effect, with soil moisture being elevated with increasing mulching thickness by 5.8%, 9.0% and 11.1% compared with CK. The soil SOC content increased by 3.0%, 2.4% and 2.3%. Although soil pH and C/N significantly (p < 0.05) increased, they fluctuated with increasing mulch thickness. Straw mulching also increased the content of >2 mm soil particle size and elevated the mean weight diameter (MWD) and geometric mean diameter (GMD). The increasing mulching thickness prolonged the effect on the stability of soil aggregates. The 4 cm maize straw mulching thickness has the best effect for ecologically and environmentally managing warm-temperate organic vineyards so it may have a great application prospect on a global scale.

Herbivory reflects the interaction between plants and insects in ecosystems, and its latitudinal patterns at the global scale have attracted widespread attention. While many studies support the latitudinal herbivory hypothesis, it remains contentious. This study, based on a global dataset of 1206 herbivory records, explored the global latitudinal patterns of insect herbivory on leaves and their influencing factors. We found that herbivory decreased with increasing latitude from the equator to the poles, supporting the latitudinal herbivory hypothesis. Latitude affected the variation in climate, soil nutrients, and plant functional traits, which ultimately affected herbivory. Plant functional traits were the key factors affecting the global latitudinal patterns of herbivory, with climatic factors playing an important regulatory role, while soil nutrients had a relatively minor impact, explaining 7.3%, 4.66%, and 0.98% of the latitudinal variation in herbivory, respectively. Specifically, plant height and mean annual temperature were the most important drivers of the global latitudinal patterns of herbivory, explaining 3.39% and 3.03%, respectively. Our study focused on two new perspectives—plant functional traits and soil nutrients. Although soil nutrients had a relatively minor influence on the latitudinal patterns of herbivory, we emphasized the significant impact of plant functional traits on the latitudinal patterns of herbivory. Our findings provide new insights into understanding and predicting the geographic patterns of herbivory and ecological interactions in the context of global climate change, offering important references and ecological significance.

This study focused on alfalfa (Medicago sativa cv. Xinmu No. 4) as the experimental material, and a two-year field plot controlled experiment was conducted to compare the effects of different co-application ratios of organic and chemical fertilizers on alfalfa yield, soil nutrient status, and soil biological characteristics. The six fertilization treatments were as follows: CM0 (100% cattle manure), CM1 (75% cattle manure + 25% chemical fertilizer), CM2 (50% cattle manure + 50% chemical fertilizer), CM3 (25% cattle manure + 75% chemical fertilizer), CM4 (100% chemical fertilizer), and CK (no fertilizer application). The results showed that alfalfa hay yield was highest under the CM3 treatment in both 2024 and 2025, representing increases of 38.03% and 40.85%, respectively, compared with the control (CK). Relative to the other treatments, CM3 significantly increased soil total nitrogen, alkali-hydrolyzable nitrogen, available phosphorus, readily available potassium, and organic matter contents. In addition, CM3 markedly enhanced the activities of soil nitrate reductase (NR), nitrite reductase (NiR), and the root enzymes glutamate synthase (GOGAT) and glutamine synthase (GS). The combined application of organic and chemical fertilizers significantly reshaped the soil bacterial community structure associated with alfalfa. Under the CM3 treatment, Chao1, Shannon, and ACE indices of soil bacterial diversity increased, whereas the Simpson index decreased. Moreover, the CM3 treatment was associated with higher relative abundances of several key bacterial phyla and genera. The 25% cattle manure plus 75% chemical fertilizer (CM3) treatment exhibited the strongest overall effects, significantly increasing total alfalfa hay yield, enhancing soil macronutrient availability and key enzyme activities, improving soil microbial α-diversity, and optimizing soil bacterial community structure. This treatment consistently outperformed the no-fertilizer control (CK) and all other organic–inorganic fertilizer combinations. Collectively, these findings provide robust scientific evidence supporting strategies to increase forage productivity, mitigate environmental impacts, and promote the sustainable development of the grassland industry.

To clarify the effects of nitrogen application rates on production performance, nutritional quality, and nitrogen use efficiency in a silage maize/sweet sorghum intercropping system, this study established a 3:3 intercropping treatment (B), a silage maize monoculture (S), sweet sorghum monoculture (T)—as well as six nitrogen application rates: 0 (N0), 120 (N1), 180 (N2), 240 (N3), 300 (N4), and 360 kg ha⁻¹ (N5). The results showed that both the cropping system and nitrogen application rate significantly influenced crop growth and yield formation. The N4 (300 kg ha⁻¹) treatment yielded the best results, promoting plant height and dry matter accumulation in both crops. Under intercropping conditions, the biomass of silage maize at the milky stage was significantly higher than that under the N0 treatment. Excessive nitrogen application (N5) did not further promote crop growth and, instead, weakened the complementary benefits of intercropping. The 3:3 intercropping combination with the N4 nitrogen application rate yielded higher forage yields and Feed Value; hay yield and crude protein yield increased by 37.86% and 52.04%, respectively, compared to N0, while crude protein content increased by 10.70%, resulting in a comprehensive production advantage over monoculture. At the same time, the N4 treatment offered a higher return on nitrogen fertilizer investment and helped maintain total nitrogen levels in the 0–40 cm soil layer. In summary, a 3:3 intercropping system of silage maize and sweet sorghum, combined with the application of 300 kg ha⁻¹ of nitrogen fertilizer, is the optimal cultivation model under the conditions of this experiment and can serve as a preliminary recommendation for optimizing nitrogen fertilizer management in grass forage intercropping systems in arid irrigation areas.

Global demand for alfalfa, a vital forage resource for ruminants, is growing. Currently, ruminant production is expanding rapidly in parts of Asia and Africa, further driving demand for high-quality alfalfa forage. Nevertheless, long-term indiscriminate fertilization and imbalanced nutrient input ratios in alfalfa production not only lower fertilizer use efficiency and crop productivity but also induce a range of soil degradation and environmental issues, thereby constraining the sustainable development of the forage-livestock industry. In this study, the alfalfa cultivar ‘Xinmu No. 4’ (Medicago sativa cv. ‘Xinmu No. 4’) was used as the experimental material. A two-year field block experiment was performed to investigate the effects of different organic–inorganic fertilizer ratios on the nutrient use efficiency of alfalfa, with six fertilization treatments: CM0 (100% cow manure), CM1 (75% cow manure + 25% chemical fertilizer), CM2 (50% cow manure + 50% chemical fertilizer), CM3 (25% cow manure + 75% chemical fertilizer), CM4 (100% chemical fertilizer), and CK (no fertilization application). The results demonstrated that the CM3 treatment (25% cow manure combined with 75% chemical fertilizer) significantly enhanced nitrogen, phosphorus, and potassium accumulation in alfalfa relative to the CK, sole cow manure (CM0), and sole chemical fertilizer (CM4) treatments (p < 0.05). Additionally, plant nutrient accumulation in 2025 was markedly higher than that in 2024 across treatments (p < 0.05). Combined organic and inorganic fertilization substantially improved the agronomic use efficiency of nitrogen, phosphorus, and potassium in alfalfa, with the CM3 ratio exhibiting the most pronounced beneficial effect. For alfalfa cultivation in Xinjiang, the combined application of 25% cow manure and 75% chemical fertilizer is recommended to optimize nutrient accumulation and utilization efficiency, ultimately boosting alfalfa yield and quality.