Microalgae-based bioremediation is increasingly recognized as a sustainable, efficient, and straightforward technology. Despite this growing interest, the potential of Parachlorella hussii for metal biosorption remains underexplored. This study is the first report evaluating the metal biosorption activity in Parachlorella hussii ACOI 1508 (N9), highlighting the impact of the culture age on the monosaccharide composition and its correlation to the metal binding capacity. The capsular strain (N9) was isolated from the hypersaline ecosystem—Lake Chott Aïn El-Beida—in southeastern Algeria. Cultivated in Bold’s Basal medium, the strain produced 0.807 ± 0.059 g L⁻¹ of RPSs and 1.975 ± 0.120 g L⁻¹ of CPSs. Biochemical analysis of the extracts revealed a high total sugar content (% w/w) that ranged from 62.98 ± 4.87% to 95.60 ± 87% and a low protein content (% w/w) that ranged from 0.49 ± 0.08% to 1.35 ± 0.69%, with RPS-D7 and RPS-D14 having high molecular weight (≥2 MDa). HPLC-based monosaccharide characterization demonstrated compositional differences between the exponential and stationary phases, with rhamnose dominating (~55%) in RPS-D14 and with the presence of uronic acids comprising 7–11.3%. Metal removal efficiency was evaluated using the whole biomass in two growth phases. Copper uptake exhibited the highest capacity, reaching 18.55 ± 0.61 mg Cu g⁻¹ DW at D14, followed by zinc removal with 6.52 ± 0.61 mg Zn g⁻¹ DW. Interestingly, removal efficiencies increased to about twofold during the stationary phase, reaching 51.15 ± 1.14% for Cu, 51.08 ± 3.35% for Zn, and 36.55 ± 3.09% for Ni. The positive results obtained for copper/zinc removal highlight the biosorption potential of P. hussii, and notably, we found that the metal removal capacity significantly improved with culture age—a parameter that has been poorly investigated in prior studies. Furthermore, we observed a growth phase-dependent modulation in monosaccharide composition, which correlated with enhanced functional properties of the excreted biomolecules involved in biosorption. This metabolic adjustment suggests an adaptive response that may contribute to the species’ effectiveness in heavy metal uptake, underscoring its novelty and biotechnological relevance.