Identifying gene regulatory networks (GRNs) at the resolution of single cells has long been a great challenge, and the advent of single-cell multi-omics data provides unprecedented opportunities to construct GRNs. Here, we propose a novel strategy to integrate omics datasets of single-cell ribonucleic acid sequencing and single-cell Assay for Transposase-Accessible Chromatin using sequencing, and using an unsupervised learning neural network to divide the samples with high copy number variation scores, which are used to infer the GRN in each gene block. Accuracy validation of proposed strategy shows that approximately 80% of transcription factors are directly associated with cancer, colorectal cancer, malignancy and disease by TRRUST; and most transcription factors are prone to produce multiple transcript variants and lead to tumorigenesis by RegNetwork database, respectively. The source code access are available at: https://github.com/Cuily-v/Colorectal_cancer.

As part of the drug repurposing process, it is imperative to predict the interactions between drugs and target proteins in an accurate and efficient manner. With the introduction of contrastive learning into drug-target prediction, the accuracy of drug repurposing will be further improved. However, a large part of DTI prediction methods based on deep learning either focus only on the structural features of proteins and drugs extracted using GNN or CNN, or focus only on their relational features extracted using heterogeneous graph neural networks on a DTI heterogeneous graph. Since the structural and relational features of proteins and drugs describe their attribute information from different perspectives, their combination can improve DTI prediction performance. We propose a relational similarity-based graph contrastive learning for DTI prediction (RSGCL-DTI), which combines the structural and relational features of drugs and proteins to enhance the accuracy of DTI predictions. In our proposed method, the inter-protein relational features and inter-drug relational features are extracted from the heterogeneous drug–protein interaction network through graph contrastive learning, respectively. The results demonstrate that combining the relational features obtained by graph contrastive learning with the structural ones extracted by D-MPNN and CNN enhances feature representation ability, thereby improving DTI prediction performance. Our proposed RSGCL-DTI outperforms eight SOTA baseline models on the four benchmark datasets, performs well on the imbalanced dataset, and also shows excellent generalization ability on unseen drug–protein pairs.

With the rapid development of Internet of Things (IoT), massive sensor data are being generated by the sensors deployed everywhere at an unprecedented rate. As the number of Internet of Things devices is estimated to grow to 25 billion by 2021, when facing the explicit or implicit anomalies in the real-time sensor data collected from Internet of Things devices, it is necessary to develop an effective and efficient anomaly detection method for IoT devices. Recent advances in the edge computing have significant impacts on the solution of anomaly detection in IoT. In this study, an adaptive graph updating model is first presented, based on which a novel anomaly detection method for edge computing environment is then proposed. At the cloud center, the unknown patterns are classified by a deep leaning model, based on the classification results, the feature graphs are updated periodically, and the classification results are constantly transmitted to each edge node where a cache is employed to keep the newly emerging anomalies or normal patterns temporarily until the edge node receives a newly updated feature graph. Finally, a series of comparison experiments are conducted to demonstrate the effectiveness of the proposed anomaly detection method for edge computing. And the results show that the proposed method can detect the anomalies in the real-time sensor data efficiently and accurately. More than that, the proposed method performs well when there exist newly emerging patterns, no matter they are anomalous or normal.

As food security gains increasing attention, automated pest monitoring is crucial for agricultural early warning systems. However, in practical light-trap capturing sensors, the extremely small scale of pests and complex background interference, such as unexpected reflection and occlusions, severely undermine the performance of existing models, resulting in frequent missed and false detections. To deal with these challenges, this study proposes EP-YOLO, an enhanced lightweight detection architecture based on YOLOv8n. Specifically, to retain the spatial pixels of micro-targets during downsampling and isolate pest features while eliminating background noise without compromising channel information, the Spatial-to-Depth Convolution (SPD) module and the Efficient Multi-Scale Attention (EMA) module are introduced. We evaluate our model through experiments on Pest24, a dataset consisting of 24 tiny pest categories. The results demonstrate that EP-YOLO achieves a mAP@50 and mAP@50:95 of 70.5% and 47.3%, respectively, improving upon the baseline by 1.1% and 1.9%. Furthermore, EP-YOLO achieves a significant improvement in detecting certain extremely small pests. For example, Rice planthopper and Plutella xylostella show improvements of 8.4% and 3.1%, respectively, compared to the baseline. In conclusion, the physical limitations of detecting tiny pests are successfully overcome by EP-YOLO, providing a robust and deployable design for real-time agricultural monitoring systems.

The stereo matching method based on a space-aware network is proposed, which divides the network into three sections: Basic layer, scale layer, and decision layer. This division is beneficial to integrate residue network and dense network into the space-aware network model. The vertical splitting method for computing matching cost by using the space-aware network is proposed for solving the limitation of GPU RAM. Moreover, a hybrid loss is brought forward to boost the performance of the proposed deep network. In the proposed stereo matching method, the space-aware network is used to calculate the matching cost and then cross-based cost aggregation and semi-global matching are employed to compute a disparity map. Finally, a disparity-post processing method is utilized such as subpixel interpolation, median filter, and bilateral filter. The experimental results show this method has a good performance on running time and accuracy, with a percentage of erroneous pixels of 1.23% on KITTI 2012 and 1.94% on KITTI 2015.