Subway stations are complex spaces with high passenger density and mobility, making evacuation efficiency particularly important. The position of evacuation signage is a key factor affecting the efficiency of passenger wayfinding. This study constructed a virtual subway station scenario by virtual reality technology (VR), recording and analyzing the evacuation time and exit selection of 60 participants. The results showed that hanging evacuation signage, with their superior visual advantages, can greatly improve passenger wayfinding efficiency, followed by signage affixed to walls and columns, and finally signage on the ground. This study has provided a theoretical basis for the scientific layout and optimization of evacuation signage positions in subway stations, thereby enhancing passenger safety and evacuation efficiency.