As terrestrial resources become increasingly scarce, the exploration and utilization of marine resources have become crucial for ensuring a stable resource supply. A maritime A-Frame is a specialized lifting mechanism mounted on the stern of a vessel, designed for deploying and retrieving heavy loads during subsea exploration. Real-time monitoring of the stress of A-Frames is essential for identifying potential failures and preventing accidents. This paper presents a stress-monitoring campaign conducted on a maritime A-Frame during a deep-sea mining project in the South China Sea. Fiber Bragg Grating (FBG) strain sensors were installed on the A-Frame to measure its stress responses throughout the deep-sea mining operation. The stress variations observed during the deployment and retrieval of a deep-sea mining vehicle were analyzed. The results indicate that the stress caused by the swinging motion of the A-Frame was significantly higher than that generated by the lifting and deployment of the mining equipment. Additionally, a finite element model (FEM) of the A-Frame was developed to estimate the stress of the hot spots by integrating the measured strain data. The analysis confirmed that the maximum stress experienced by the A-Frame was well below the allowable threshold, indicating that the structure had sufficient strength to withstand operational loads. In addition, the swing angle of the A-Frame significantly affects the stress value of the A-Frame, while lifting the mining vehicle has a very slight effect. Thus, it is advisable to accelerate the deployment and retrieval speeds of the mining vehicle and minimize the outward swing angle of the A-Frame. These findings provide valuable insights for optimizing the design and ensuring the safe operation of maritime A-Frames in deep-sea mining exploration.