Antibiotic resistance is a global concern for human, animal, and environmental health. Many studies have identified wastewater treatment plants and surface waters as major reservoirs of antibiotic resistant bacteria (ARB) and genes (ARGs). Yet their prevalence in urban karst groundwater systems remains largely unexplored. Considering the extent of karst groundwater use globally, and the growing urban areas in these regions, there is an urgent need to understand antibiotic resistance in karst systems to protect source water and human health. This study evaluated the prevalence of ARGs associated with resistance phenotypes at 10 urban karst features in Bowling Green, Kentucky weekly for 46 weeks. To expand the understanding of prevalence in urban karst, a spot sampling of 45 sites in the Tampa Bay Metropolitan area, Florida was also conducted. Specifically, this study considered tetracycline and extended spectrum beta-lactamase (ESBLs) producing, including third generation cephalosporin, resistant E. coli, and tetracycline and macrolide resistant Enterococcus spp. across the 443 Kentucky and 45 Florida samples. A consistent prevalence of clinically relevant and urban associated ARGs were found throughout the urban karst systems, regardless of varying urban development, karst geology, climate, or landuse. These findings indicate urban karst groundwater as a reservoir for antibiotic resistance, potentially threatening human health.

Streamflow monitoring for flood warning and watershed management applications in the United States is a cost-intensive venture, and usually performed by government agencies such as the US Geological Survey (USGS). With reduced resources across the federal agencies towards environmental monitoring, agencies and stakeholders are challenged to respond with cross-cutting, collaborative, and low-cost alternatives for streamflow monitoring. One such alternative is using low-cost environmental sensors and developing a real-time gage/sensor network using IoT (Internet of Things) devices. With this technology, smaller watersheds (e.g., HUC-8 and HUC-10 level) can be equipped with low-cost gages at many locations and a clear picture of the hydrological response can be obtained. This paper presents the development and implementation of a low-cost real-time water monitoring network for the Falling Water River (FWR) watershed in the middle Tennessee region in the US. To develop and implement this gage network, the following three tasks were performed: (i) assemble a low-cost, real-time internet enabled water level gage, (ii) field-test the sensor prototype and, (iii) deploy the sensors and build a network. A collaborative partnership was developed with stakeholders including the Tennessee Department of Environment and Conservation, Tennessee Department of Transportation, Burgess Falls State Park, City of Cookeville, and Friends of Burgess Falls. The performance of the gages in water level estimation was compared with the water levels measured with a nearby USGS streamgage. The comparison was performed for the 2020–2022 time period and at two levels: event-based comparison and a long-term comparison. Nine storm events were selected for the comparison, which showed “Very Good” agreement in terms of Coefficient of Determination (R²), Nash–Suttcliffe Efficiency (NSE), and percent bias (PBIAS) (except for four events). The mean squared error (MSE) ranged between 0.07 and 1.06 while the root mean squared error (RMSE) ranged between 3 inches and 12 inches. A long-term comparison was performed using Wilcoxon Signed-Rank test and Loess Seasonal Decomposition analysis, which showed that the differences between the two datasets is not significant and that they trended well across the two year period. The gages are currently installed along the main channel and tributaries of the Falling Water River, which also include portions of the Window Cliffs State Natural Area. With continued support from the stakeholders, the number of sensors are projected to increase, resulting in a dense sensor network across the watershed. This will over time enable the stakeholders to have a spatially variable hydrological response of the Falling Water River Watershed.

Place-based learning is an educational approach that centers around the concept of place. Intersecting watershed literacy with place-based education methods, this study explores a short-term place-based watershed outreach program in a rural high school in Tennessee, United States. As the community’s first known watershed outreach program, this pilot study aimed to improve the watershed literacy of its student participants by engaging them in the locally relevant and shared phenomenon of flooding. Overall, five units were developed, with each providing a unique interactive lecture and activity. The program’s short-term effectiveness in improving watershed literacy was evaluated anonymously through pre- and post-program assessments, as well as pre- and post-unit assessments. Ultimately, the program resulted in moderate short-term improvements in student watershed literacy, as measured by pre- and post-program surveys. The program content assessments resulted in an average percent change in watershed literacy of +34%. A Likert scale survey indicated that the students perceived an increase in watershed connectedness and confidence, but a decreased willingness to address watershed stormwater and flooding issues. Additional assessments are required to evaluate the long-term impacts of this outreach.