Recent advances in DNA research have increased the necessity for museums to preserve not only morphological specimens but also their DNA, leading us to maintain tissue samples linked to specimens at −80 °C. DNA analysis has become an essential tool for taxonomic research and biodiversity assessment; however, freezer storage for all samples is impractical due to space limitations and operational costs. This creates a pressing need to develop more widely applicable DNA preservation methods. We investigated the comparative effects of traditional preservation methods versus DESS (DMSO/EDTA/saturated NaCl solution) preservation on both morphology and DNA integrity using museum specimens from various taxonomic groups. Our results demonstrated that DESS preservation maintained high-quality DNA fragments exceeding >15 kb at room temperature across all examined species, with nematode samples maintaining DNA integrity even after 10 years of storage. When preserving whole organisms, the optimal preservation solution conditions for maintaining both morphological features and DNA integrity varied among species. Notably, DNA integrity was maintained even after complete evaporation of the DESS solution. These findings suggest that DESS utilization for specimen DNA preservation is effective across many species, not only for long-term storage in environments without freezer facilities but also for temporary preservation until freezing.

Taxonomic knowledge provides a scientific name to each organismal group and is thus indispensable information for understanding biodiversity. However, the various perspectives of classifying organisms and changes in taxonomic knowledge have led to in

The Adoxophyes tea tortrix (Lepidoptera: Tortricidae) is a group of leaf rollers that cause enormous economic losses on tea and apple crops. In East Asia, taxonomic ambiguity of the Adoxophyes orana complex (AOC), which consists of A. orana, A. dubia, A. honmai, and A. paraorana, has persisted for decades because of vague diagnostic characters. In this study, differences in the AOC were examined to improve species identification, determine genetic variations, and develop control strategies. Analyses revealed that A. orana comprised 2 lineages, a widely distributed Palearctic lineage and an East Asian lineage that was nested with other Asian species. Genetic divergence of >3% is proposed to confirm the AOC species that would benefit subsequent taxonomic revision. The monophyletic Taiwanese A. sp. with 2.8–4% from other AOC species appeared to suggest it as an independent taxon, and low interspecific divergence between A. honmai and A. dubia of 0.3% indicated possibility of recent divergence or intraspecific variations. Our result further suggested that the Z9-14:Ac ratio of semiochemicals could be a reference for the reblending of pheromone attractants in Taiwanese tea plantations. Moreover, the AOC species appeared to have a tendency of specific geographic distributions, with A. dubia and A. honmai in Japan and China, A. paraorana in Korea, and A. sp. in Taiwan. Maintaining the unique genetic composition of Adoxophyes species in each geographic region and preventing the possible invasions into those AOC-free countries through the transportation of host plants are essential in managing the AOC in East Asia.

Lepidoptera specimens deposited at the Department of Zoology, National Museum of Nature and Science

Neuroptera specimens deposited at the Department of Zoology, National Museum of Nature and Science

Language localization is a process of adapting the needs of a certain linguistic community, including its cultural contexts, to adjust the nuance and wording of the original language. It is not just a translation from English, the de facto language of publication in the scientific community. This is particularly important in a linguistically diverse region such as Asia, including Japan, and thus language localization is crucial for developing an environment that enables the collection and utilization of biodiversity-related data in their first language. The Japan Biodiversity Information Initiative (JBIF) is an organization aiming to carry out activities of the Global Biodiversity Information Facility (GBIF) in Japan. In this presentation, we will introduce our experiences of language localization in JBIF, which includes improving access, adapting Japanese names (a kind of vernacular name), and images. JBIF has been working to improve the Japanese community’s access to GBIF data, translating the GBIF website, and a variety of other activities. These works are related to one of our missions, “utilization of biodiversity data.” Furthermore, Science Museum Net (S-Net), a portal site operated by the National Museum of Nature and Science (NMNS), has been collecting natural history specimen data from research institutions and museums, including NMNS itself; has disseminated data through GBIF; and has provided data searchability for domestic users. Language localization has contributed to internationally disseminating biodiversity information by improving access to global platforms. S-Net has adopted a data format that includes items written in Japanese and corresponding occurrence terms in the Darwin Core Standard (Darwin Core Task Group 2009), as well as several unique fields requested by domestic users. S-Net also designed the data entry flow, which allows the entire process—from submission to domestic and/or international publication—to be completed in Japanese. With this data entry process, S-Net has collected more than 7.3 million data records, the largest number in Asia, from more than 100 museums and institutions over the past 20 years. Along with government support, language localization inevitably helps reduce barriers for many participants, including small museums with limited capacity to submit specimen data. In this context, language localization is particularly crucial for the treatment of species names. In the Japanese community, many organisms have “Japanese names” that corresponds to each taxon. As Japanese names are familiar to both the scientific community and citizens in Japan, Japanese names are more widely used than scientific names. Therefore, scientific and citizen communities must be able to access biodiversity information using Japanese names. JBIF has provided a service to the GBIF database by converting the Japanese name to a scientific name using the species name dictionary. This required the development of a species list that includes Japanese names in addition to scientific names and serves as the backbone of the database. This localization could also apply to several Asian countries where there is a vernacular name system similar to that of Japan. Language localization also plays an important role in developing databases that include accompanying images with biodiversity information, a growing trend in Japan. With the amendment of the Museum Act in 2023, museums in Japan are now required to digitize and publish specimens and materials in their possession. By publishing and sharing specimen images, various uses beyond science are expected. Some museums, including NMNS, already have databases that can publish accompanying images with text data, while other museums, especially small museums, do not. S-Net is expected to add a function to publish images. S-Net is also connected to an integrated platform for searching aggregated metadata and images in various fields in Japan, such as data from museums, galleries, and libraries. Images should also contribute to improving the accuracy of biodiversity-related data by complementing language localization. During digitization, typing errors and misinterpretations may occur, and raw data such as handwritten labels may be lost. Publication of such accompanying information in images, other than the specimen itself, should allow data users to validate accuracy and identify potential errors to be corrected. S-Net should expand its role from improving image datasets by developing an image release system. To achieve this, it is essential to cultivate a culture of Findability, Accessibility, Interoperability and Reuse (FAIR) and open data, and to establish Creative Commons licensing and data handling protocols among stakeholders in Japan. We believe that our work serves as a valuable precedent for collection and dissemination of biodiversity information from Asia, and for fostering collaboration among research communities, citizens, and governments at appropriate scales in Asian countries and the rest of the world.