One strategy for improving fertility in cattle is administration of GnRH or human chorionic gonadotropin (hCG) during the luteal phase, which increases progesterone (P4) secretion and delays luteolysis. To provide an overview of how GnRH or hCG treatment between 4 and 15 d after artificial insemination (AI) improves pregnancy per AI (P/AI) in cows, a meta-analysis was performed on 107 different trials from 52 publications. Data from 18,082 treated cows and 18,385 untreated controls were meta-analyzed. The meta-analysis explained the relative risk for P/AI with GnRH or hCG treatment under various circumstances. The results did not show any difference in P/AI between cows treated with hCG and cows treated with GnRH. Compared with no treatment, treatment with GnRH or hCG improved the chances of P/AI in cows with very poor (60.1%). Moreover, treatment with GnRH and hCG improved the chances of P/AI in primiparous cows. The improvement was much better in primiparous cows with very low fertility. Treatment with buserelin at a dose above 10 µg and with hCG at a dose above 2,500 IU was associated with increased chances of P/AI compared with lower doses. Treatment with GnRH 10 d after AI was also associated with increased chances of P/AI compared with earlier treatment. The present meta-analysis showed that the use of GnRH and hCG after AI should be focused on cows expected to have low or moderate fertility. Day and dose of treatment have to be considered as well.

Eight sandfly-borne phleboviruses were found to circulate in North Africa. Phleboviruses detected in sandflies were Toscana (TOSV), Sandfly Fever Sicilian (SFSV), Sandfly Fever Naples (SFNV), Cyprus (CYPV), Punique (PUNV), Utique, Saddaguia, and Medjerda Valley (MVV) viruses, yielding an overall infection rate of 0.02–0.6%. Phlebotomus perniciosus and Phlebotomus longicuspis were the most common vector species in the region. TOSV seroprevalence in dogs from Algeria (4.56%) and Tunisia (7.5%) was low and close, unlike SFSV (38.1%) and PUNV (43.5%), which were restricted to Tunisia. SFSV (1.3–21%) and TOSV (3.8–50%) were the most prevalent among humans. TOSV was frequently detected and symptomatically confirmed in both Algeria (3.8%) and Tunisia (12.86%). Other sandfly-borne phleboviruses have also been detected but less importantly, such as SFNV in Morocco (2.9%) and Tunisia (1.1%) and PUNV (8.72%), CYPV (2.9%), and MVV (1.35%) in Tunisia. Their distribution was mainly northern. Overall, 15.9% of the healthy population were seropositive for sandfly-borne phleboviruses, with evidenced cocirculation. Noticeably, studies conducted in Morocco were mostly interested in TOSV in sandflies. Available data from Libya and Egypt were scant or historical. Further elaboration is required to check the sporadic detection of less-prevalent phleboviruses and fully elucidate the epidemiological situation.

Background and Aim: Ultrasonographic fetal sexing is of utmost economic importance for horse breeders. Relatively, a few studies have been conducted to determine fetal sex in mare using transrectal Doppler ultrasound. This study aimed to compare two sexing techniques, two-dimensional (2D) mode and color Doppler ultrasonography. Materials and Methods: The study was conducted on 39 mares under field conditions. Examinations were performed using the ultrasonic model device (Medison SonoAce Pico, South Korea), equipped with real-time 3-7 MHz convex multifrequency transducer. Fetal sex diagnosis was carried out in two periods of pregnancy, early period (57-80 days of gestation) and late period (80-150 days of gestation). Results: No difference (p=0.4) was observed between the efficiency of the 2D mode and Doppler ultrasound in sex determination with the respective frequency of 74% and 85%. The best time to sex the early fetus was between 57 and 70 days of gestation and between 90 and 120 days for the older fetus. Conclusion: The accuracy of sex determination can be increased using Doppler mode compared to 2D mode, especially in the early period between 57 and 70 days, in male more than female sex.

Background and Aim: Marek's disease (MD) is a lymphoproliferative disease that occurs in chickens. In the absence of control measures, MD causes devastating losses to commercial poultry flocks. Vaccination has enabled dramatic success in the prevention and control of MD. However, the MD vaccination program has failed frequently, and occasional clinical outbreaks have been reported in the vaccinated flocks as well. The present study aimed to describe the clinical and histopathological characteristics of the field cases of MD in broiler breeder flocks. Materials and Methods: A survey on the update of MD occurrence in Algerian broiler breeder flocks was conducted from June 2020 to September 2020. Ten vaccinated broiler breeder flocks located in Central Algeria and having progressive tumors in different visceral organs were evaluated for MD virus infection by conducting a histopathological examination of the birds. Results: The age of the birds affected with MD ranged from 13 to 22 weeks. The mortality rate varied sensitively from 4% to 10%. The clinical symptoms reported in the affected flocks included locomotor, nervous, digestive, and respiratory symptoms. Necropsy of the dead or euthanized birds revealed visceral lymphomatosis in several organs and macroscopic changes in the peripheral nerves (including loss of longitudinal striation, color change [grayish], and volume increase). The histopathological findings included the infiltration and proliferation of lymphocytes and blast cells (lymphoblasts) in various organs of the birds, which are the typical characteristics of MD and, therefore, confirmed the field infection of MD in these birds. Conclusion: The present study provided evidence for the high prevalence of MD in the broiler breeder flocks vaccinated with a bivalent vaccine (turkey herpesvirus+Rispens) at the hatchery. The findings of the present study may indicate high-level failure of vaccination in these birds.

Elevated ammonia and urea, common byproducts of nitrogen metabolism, are increasingly found in dairy cows and may negatively impact reproductive function. However, their effects on bull sperm remain unclear. This study aimed to assess the impact of ammonia and urea at physiologically relevant concentrations on key sperm characteristics. Thawed bull semen was incubated under five treatment conditions: control (no added ammonia or urea), low urea (LU), high urea (HU), low ammonia (LA), and high ammonia (HA). Sperm motility and kinematics were assessed using Computer-Assisted Semen Analysis (CASA), and sperm viability, DNA integrity, mitochondrial membrane potential (MMP), and oxidative stress markers were evaluated using flow cytometry. HA significantly reduced MMP (p = 0.008) and several motility parameters, including progressive motility and velocity (VCL, VSL, ALH), compared to LA and control groups. A decrease in sperm viability was observed in the HA group compared to LA at the beginning of incubation. While HU reduced MMP (p = 0.002), sperm motility was not significantly affected compared to LU. No significant differences were found in DNA fragmentation or oxidative stress biomarkers between groups. These results highlight the impact of ammonia, specifically, on sperm mitochondrial function and motility, which are crucial for successful fertilization.