The capacity of carbon sinks varies among the different types of ecosystems, and whether national parks, as an important type of nature reserve, have a high carbon sink capacity (CSC) and whether eco-tourism in national parks affects their CSC are the main scientific issues discussed. Using MODIS Net Primary Production (NPP) product data, this study analysed the spatiotemporal variation in carbon sources and sinks (CSSs) in the ecosystem of Huangshan National Park from 2000 to 2020, as well as the impact of tourism on these carbon sources and sinks. The findings indicate that, while the ecosystems of national parks generally have a strong CSC, they may not always function as carbon sinks, and during the study period, Huangshan National Park served as a carbon source for four years. Temporally, the CSSs in the ecosystem of the national park exhibit a cyclical pattern of change with a four-year cycle and strong seasonality, with spring and autumn functioning as carbon sinks, and summer and winter as carbon sources. Spatially, the CSSs of the national park ecosystem exhibited a vertical band spectrum of spatial distribution, and the CSC showed a trend of gradual enhancement from low altitude to high altitude. Tourism is a major factor that has an impact on the CSC of national park ecosystems.