Long non-coding RNAs (lncRNAs) have been found to play a vital role in several gene regulatory networks involved in the various biological processes in plants related to stress response. However, systematic analyses of lncRNAs expressed in rice Cadmium (Cd) stress are seldom studied. Thus, we presented the characterization and expression of lncRNAs in rice root development at an early stage in response to Cd stress. The lncRNA deep sequencing revealed differentially expressed lncRNAs among Cd stress and normal condition. In the Cd stress group, 69 lncRNAs were up-regulated and 75 lncRNAs were down-regulated. Furthermore, 386 matched lncRNA-mRNA pairs were detected for 120 differentially expressed lncRNAs and 362 differentially expressed genes in cis, and target gene-related pathway analyses exhibited significant variations in cysteine and methionine metabolism pathway-related genes. For the genes in trans, overall, 28,276 interaction relationships for 144 lncRNAs and differentially expressed protein-coding genes were detected. The pathway analyses found that secondary metabolites, such as phenylpropanoids and phenylalanine, and photosynthesis pathway-related genes were significantly altered by Cd stress. All of these results indicate that lncRNAs may regulate genes of cysteine-rich peptide metabolism in cis, as well as secondary metabolites and photosynthesis in trans, to activate various physiological and biochemical reactions to respond to excessive Cd. The present study could provide a valuable resource for lncRNA studies in response to Cd treatment in rice. It also expands our knowledge about lncRNA biological function and contributes to the annotation of the rice genome.

Mapping major quantitative trait loci (QTL) responsible for rice seed germinability under low temperature (GULT) can provide valuable gene sources for cold tolerant rice lines breeding. In this study, 124 rice backcross recombinant inbred lines (BRILs) derived from indica cv. Changhui 891 and japonica cv. 02428 were genotyped through re-sequencing technology, and a bin map including 3057 bins spanning 1266.5 cM with an average resolution of 0.41 cM between markers was generated. Based on the new high-density genetic map, six QTL ranged from 40 kb to 140 kb in Nipponbare genome were fine-mapped. Among these, qCGR8 and qGRR11, whose 02428 alleles could increase GULT and seed germination recovery rate after cold stress, respectively, were detected. qNGR1 and qNGR4 may be two major QTL affecting indica Changhui 891 germination under normal condition. qGRR1 and qGRR8 are two QTL affecting the seed germination recovery rate after cold stress, the Changhui 891 alleles can improve seed germination rate after cold stress dramatically. These QTL would be a highly valuable genetic factor for cold tolerant rice lines breeding. Moreover, the BRILs developed in this study serve as an excellent system for mapping and studying genetic basis of rice complex traits.

Multidrug and toxic compound extrusion (MATE) proteins are involved in many physiological functions of plant growth and development. Although an increasing number of MATE proteins have been identified, the understanding of MATE proteins is still very limited in rice. In this study, 46 MATE proteins were identified from the rice (Oryza sativa) genome by homology searches and domain prediction. The rice MATE family was divided into four subfamilies based on the phylogenetic tree. Tandem repeats and fragment replication contribute to the expansion of the rice MATE gene family. Gene structure and cis-regulatory elements reveal the potential functions of MATE genes. Analysis of gene expression showed that most of MATE genes were constitutively expressed and the expression patterns of genes in different tissues were analyzed using RNA-seq. Furthermore, qRT-PCR-based analysis showed differential expression patterns in response to salt and drought stress. The analysis results of this study provide comprehensive information on the MATE gene family in rice and will aid in understanding the functional divergence of MATE genes.

N⁶-methyladenosine (m⁶A) is the most prevalent internal modification present in the mRNAs of all higher eukaryotes. However, the role of the m⁶A methylomes in rice is still poorly understood. With the development of the MeRIP-seq technique, the in-depth identification of mRNAs with m⁶A modification has become feasible. A study suggested that m⁶A modification is crucial for posttranscriptional regulation related to Cd²⁺-induced malignant transformation, but the association between m⁶A modification in plants and Cd tolerance has not been reported. We investigated the m⁶A methylomes in the roots of a cadmium (Cd)-treated group and compared them with the roots in the control (CK) group by m⁶A sequencing of cv. 9311 and cv. Nipponbare (NIP) plants. The results indicated that Cd leads to an altered modification profile in 3,406 differential m⁶A peaks in cv. 9311 and 2,065 differential m⁶A peaks in cv. NIP. KEGG pathway analysis of the genes with differentially modified m⁶A peaks indicated that the “phenylalanine”, “tyrosine and tryptophan biosynthesis”, “glycine”, “adherens junctions”, “glycerophospholipid metabolism” and “threonine metabolism” signalling pathways may be associated with the abnormal root development of cv. 9311 rice due to exposure to Cd. The “arginine”, “proline metabolism”, “glycerolipid”, and “protein processing in endoplasmic reticulum” metabolism pathways were significantly enriched in genes with differentially modified m⁶A peaks in cv. NIP. Unlike that in Arabidopsis, the m⁶A-modified nucleotide position on mRNAs (m⁶A peak) distribution in rice exhibited a preference towards both the stop codon and 3′ untranslated regions (3′ UTRs). These findings provide a resource for plant RNA epitranscriptomic studies and further increase our knowledge on the function of m⁶A modification in RNA in plants.