This study resulted from the demand for seeds with physiological qualities and studies in germination tests applied for seed improvement aimed at productive and homogeneous harvests. The objective of this study was to improve the classification of seeds in germination tests by introducing YOLO as a classification tool for germinated or nongerminated seeds to specify the results and optimize the analysis period. Germination tests were performed for Glycine max (soybean) seeds, and the capture of images from the tests and conventional categorization was performed by uncorrelated individuals, for the processing of these images and application to YOLO. Subsequently, graphical analyses of the YOLO results and comparison metrics with conventional categorization were performed to determine the accuracy of YOLO as a seed categorization tool. The results derived from the analysis of the graphs and comparisons to the conventional methodology of seed classification showed the effectiveness of YOLO for classifying seeds as germinated or nongerminated, reaching 95% accuracy in seed classification, beyond the range of 0–0.110 of the prediction errors, determined by the application of the methodology of mean square error, highlighting the efficiency of YOLO.