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Dr. Kamila Domińska
Medical University of Lodz, Department of Comparative Endocrinology,

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renin-angiotensin system
Ang II
relaxin
Ang-(1-7)
Ang-(1-9)
Ang-(3-7)
reproductive cancers

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Journal article
Published: 12 January 2021 in International Journal of Molecular Sciences
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Mycotoxins are present in everyday diet as common food and feed pollutants. A part of them is still concerned as so-called emerging mycotoxins. Due to the lack of toxicity data, the safety limits and detail molecular mechanism have been not established yet for all of them. Alternariol (AOH), as one of these mycotoxins, produced by Alternaria species, is so far reported as an estrogenic, genotoxic, and immunomodulatory agent; however, its direct effect on human health is not known. Especially, in the case of hormone-dependent tissues which are sensitive to both endogenic, as well as external estrogenic agents, it might be crucial to assess the effect of AOH. Thus, this study evaluated how exposure to AOH affects viability and motility of the human normal mammary gland epithelial in vitro model. We observed that AOH significantly affects viability of cells in a time- and dose-dependent manner. Moreover, the induction of oxidative stress, DNA damage, and cell cycle arrest in the G2/M cell cycle phase was observed. The motility of 184A1 cells was also significantly affected. On the molecular level, AOH induced antioxidative stress response via activation of Nuclear factor erythroid 2-related factor 2 (NRF2) signaling pathway agents, as well as decrease in the phosphorylation of protein kinase B (Akt) and p44/42 (ERK 1-2) molecules, indicating that AOH might affect crucial signaling pathways in both physiological and pathophysiological processes in breast tissue.

ACS Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Marta Justyna Kozieł; Kinga Anna Urbanek; Kamila Domińska; Agnieszka Wanda Piastowska-Ciesielska. Mycotoxin Alternariol (AOH) Affects Viability and Motility of Mammary Breast Epithelial Cells. International Journal of Molecular Sciences 2021, 22, 696 .

AMA Style

Karolina Kowalska, Dominika Ewa Habrowska-Górczyńska, Marta Justyna Kozieł, Kinga Anna Urbanek, Kamila Domińska, Agnieszka Wanda Piastowska-Ciesielska. Mycotoxin Alternariol (AOH) Affects Viability and Motility of Mammary Breast Epithelial Cells. International Journal of Molecular Sciences. 2021; 22 (2):696.

Chicago/Turabian Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Marta Justyna Kozieł; Kinga Anna Urbanek; Kamila Domińska; Agnieszka Wanda Piastowska-Ciesielska. 2021. "Mycotoxin Alternariol (AOH) Affects Viability and Motility of Mammary Breast Epithelial Cells." International Journal of Molecular Sciences 22, no. 2: 696.

Journal article
Published: 28 August 2020 in International Journal of Molecular Sciences
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The local renin–angiotensin system (RAS) plays an important role in the pathophysiology of the prostate, including cancer development and progression. The Ang-(1-9) and Ang-(3-7) are the less known active peptides of RAS. This study examines the influence of these two peptide hormones on the metabolic activity, proliferation and migration of prostate cancer cells. Significant changes in MTT dye reduction were observed depending on the type of angiotensin and its concentration as well as time of incubation. Ang-(1-9) did not regulate the 2D cell division of either prostate cancer lines however, it reduced the size of LNCaP colonies formed in soft agar, maybe through down-regulation of the HIF1a gene. Ang-(3-7) increased the number of PC3 cells in the S phase and improved anchorage-independent growth as well as mobility. In this case, a significant increase in MKI67, BIRC5, and CDH-1 gene expression was also observed as well as all members of the NF-kB family. Furthermore, we speculate that this peptide can repress the proliferation of LNCaP cells by NOS3-mediated G2/M cell cycle arrest. No changes in expression of BIRC5 and BCL2/BAX ratio were observed but a decrease mRNA proapoptotic BAD gene was seen. In the both lines, Ang-(3-7) improved ROCK1 gene expression however, increased VEGF and NOS3 mRNA was only seen in the PC3 or LNCaP cells, respectively. Interestingly, it appears that Ang-(1-9) and Ang-(3-7) can modulate the level of steroidogenic enzymes responsible for converting cholesterol to testosterone in both prostate cancer lines. Furthermore, in PC3 cells, Ang-(1-9) upregulated AR expression while Ang-(3-7) upregulated the expression of both estrogen receptor genes. Ang-(1-9) and Ang-(3-7) can impact on biological properties of prostate cancer cells by modulating inflammatory and steroidogenesis pathway genes, among others.

ACS Style

Kamila Domińska; Karolina Kowalska; Kinga Anna Urbanek; Dominika Ewa Habrowska-Górczyńska; Tomasz Ochędalski; Agnieszka Wanda Piastowska Ciesielska. The Impact of Ang-(1-9) and Ang-(3-7) on the Biological Properties of Prostate Cancer Cells by Modulation of Inflammatory and Steroidogenesis Pathway Genes. International Journal of Molecular Sciences 2020, 21, 6227 .

AMA Style

Kamila Domińska, Karolina Kowalska, Kinga Anna Urbanek, Dominika Ewa Habrowska-Górczyńska, Tomasz Ochędalski, Agnieszka Wanda Piastowska Ciesielska. The Impact of Ang-(1-9) and Ang-(3-7) on the Biological Properties of Prostate Cancer Cells by Modulation of Inflammatory and Steroidogenesis Pathway Genes. International Journal of Molecular Sciences. 2020; 21 (17):6227.

Chicago/Turabian Style

Kamila Domińska; Karolina Kowalska; Kinga Anna Urbanek; Dominika Ewa Habrowska-Górczyńska; Tomasz Ochędalski; Agnieszka Wanda Piastowska Ciesielska. 2020. "The Impact of Ang-(1-9) and Ang-(3-7) on the Biological Properties of Prostate Cancer Cells by Modulation of Inflammatory and Steroidogenesis Pathway Genes." International Journal of Molecular Sciences 21, no. 17: 6227.

Review
Published: 27 June 2020 in International Journal of Molecular Sciences
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In addition to the classic, endocrine renin-angiotensin system, local renin-angiotensin system (RAS) has been documented in many tissues and organs, including the ovaries. The localization and functional activity of the two opposing axes of the system, viz. ACE1/Ang II/AT1 and ACE2/Ang-(1-7)/MAS1, differs between animal species and varied according to the stage of follicle development. It appears that the angiotensin peptides and their receptors participate in reproductive processes such as folliculogenesis, steroidogenesis, oocyte maturation, and ovulation. In addition, changes in the constituent compounds of local RAS may contribute to pathological conditions, such as polycystic ovary syndrome, ovarian hyperstimulation syndrome, and ovarian cancer. This review article examines the expression, localization, metabolism, and activity of individual elements of the ACE2/Ang-(1-7)/MAS1 axis in the ovaries of various animal species. The manuscript also presents the relationship between the secretion of gonadotropins and sex hormones and expression of Ang-(1-7) and MAS1 receptors. It also summarizes current knowledge regarding the positive and negative impact of ACE2/Ang-(1-7)/MAS1 axis on ovarian function.

ACS Style

Kamila Domińska. Involvement of ACE2/Ang-(1-7)/MAS1 Axis in the Regulation of Ovarian Function in Mammals. International Journal of Molecular Sciences 2020, 21, 4572 .

AMA Style

Kamila Domińska. Involvement of ACE2/Ang-(1-7)/MAS1 Axis in the Regulation of Ovarian Function in Mammals. International Journal of Molecular Sciences. 2020; 21 (13):4572.

Chicago/Turabian Style

Kamila Domińska. 2020. "Involvement of ACE2/Ang-(1-7)/MAS1 Axis in the Regulation of Ovarian Function in Mammals." International Journal of Molecular Sciences 21, no. 13: 4572.

Original article
Published: 20 June 2020 in Cell Biology and Toxicology
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Background Methylsulfonylmethane (MSM) is a commonly used diet supplement believed to decrease the inflammation in joints and fastens recovery in osteoarthritis, gastric mucosal injury, or obesity-related disorders. It was also suggested that MSM might play a beneficial role in cancer treatment. Purpose So far, the MSM might have a potentially beneficial effect in endometrial cancer (EC) treatment. Study design This study evaluated the effect and usefulness of MSM in combinatory therapy with known drug doxorubicin (DOX). Methods The effect of combinational treatment of MSM and DOX on the induction of apoptosis was evaluated in EC cell lines (ISHIKAWA, MFE-296, MFE-280). Results We observed that MSM itself induces apoptosis in EC cell lines, and pre-treatment with MSM for 24 h increases the sensitivity of EC cells to DOX-induced apoptosis and DNA damage and that effect might be regulated by p42/44 (Erk1/2) MAPK and Akt (protein kinase B). Conclusion These results for the first time show that MSM might act as a sensitizer of EC cells to known drugs, for which EC cells quickly acquire resistance.

ACS Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Dominika Kurczewska; Kamila Domińska; Kinga Anna Urbanek; Agnieszka Wanda Piastowska-Ciesielska. Methylsulfonylmethane sensitizes endometrial cancer cells to doxorubicin. Cell Biology and Toxicology 2020, 37, 261 -275.

AMA Style

Karolina Kowalska, Dominika Ewa Habrowska-Górczyńska, Dominika Kurczewska, Kamila Domińska, Kinga Anna Urbanek, Agnieszka Wanda Piastowska-Ciesielska. Methylsulfonylmethane sensitizes endometrial cancer cells to doxorubicin. Cell Biology and Toxicology. 2020; 37 (2):261-275.

Chicago/Turabian Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Dominika Kurczewska; Kamila Domińska; Kinga Anna Urbanek; Agnieszka Wanda Piastowska-Ciesielska. 2020. "Methylsulfonylmethane sensitizes endometrial cancer cells to doxorubicin." Cell Biology and Toxicology 37, no. 2: 261-275.

Journal article
Published: 22 March 2020 in Toxins
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Nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) is commonly expressed in prostate cancer (PCa) cells and is associated with increased proliferation, metastases and androgen independence. Zearalenone (ZEA) is one of the most common mycotoxins contaminating food, which might mimic estrogens and bind to estrogen receptors (ERs). The ratio of androgens to estrogens in men decreases physiologically with age, and is believed to participate in prostate carcinogenesis. In this study, we evaluated the role of NFκB and ERβ in the induction of oxidative stress in human PCa cells by ZEA. As observed, ZEA at a dose of 30 µM induces oxidative stress in PCa cells associated with DNA damage and G2/M cell cycle arrest. We also observed that the inhibition of ERβ and NFΚB via specific inhibitors (PHTPP and BAY 117082) significantly increased ZEA-induced oxidative stress, although the mechanism seems to be different for androgen-dependent and androgen-independent cells. Based on our findings, it is possible that the activation of ERβ and NFΚB in PCa might protect cancer cells from ZEA-induced oxidative stress. We therefore shed new light on the mechanism of ZEA toxicity in human cells.

ACS Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kamila Domińska; Kinga Anna Urbanek; Agnieszka Wanda Piastowska-Ciesielska. ERβ and NFκB—Modulators of Zearalenone-Induced Oxidative Stress in Human Prostate Cancer Cells. Toxins 2020, 12, 199 .

AMA Style

Karolina Kowalska, Dominika Ewa Habrowska-Górczyńska, Kamila Domińska, Kinga Anna Urbanek, Agnieszka Wanda Piastowska-Ciesielska. ERβ and NFκB—Modulators of Zearalenone-Induced Oxidative Stress in Human Prostate Cancer Cells. Toxins. 2020; 12 (3):199.

Chicago/Turabian Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kamila Domińska; Kinga Anna Urbanek; Agnieszka Wanda Piastowska-Ciesielska. 2020. "ERβ and NFκB—Modulators of Zearalenone-Induced Oxidative Stress in Human Prostate Cancer Cells." Toxins 12, no. 3: 199.

Short communication
Published: 25 September 2019 in Biochemical and Biophysical Research Communications
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There is growing evidence that renin-angiotensin system (RAS) components have been involved in the development of various types of cancers, including prostate cancer. This article for the first time reports the impact of Ang1-9 and Ang3-7 on viability and proliferation, migration and invasion of epithelial prostate cells. The results of this study clearly show that Ang1-9 and Ang3-7 exert different/opposite effects on in vitro biological properties of prostate cells. It appears that Ang1-9 has pro-cancer activities via the ability to induce cell divisions, enhance cell motility and stimulate the expression of such genes as vascular endothelial growth factor (VEGF), hypoxia-inducible factors (HIF-1), vimentin (VIM) and REL proto-oncogene, NF-kB subunit (REL). On the contrary, Ang3-7 did not show any mitogenic activity. Furthermore, this peptide hormone limited the migration of PNT1A cells probably by downregulation of VEGF and VIM expression. Finally, it is worth noting that both angiotensins have the ability to modulate gene expression for angiotensin receptors. Unfortunately, we could not unequivocally identify the type of angiotensin receptor responsible for signal transduction pathway involved in PNT1A cell survival and proliferation. Undoubtedly, further research and testing in this area are necessary.

ACS Style

Kamila Domińska; Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kinga Anna Urbanek; Tomasz Ochędalski; Agnieszka Wanda Piastowska-Ciesielska. The opposite effects of angiotensin 1-9 and angiotensin 3-7 in prostate epithelial cells. Biochemical and Biophysical Research Communications 2019, 519, 868 -873.

AMA Style

Kamila Domińska, Karolina Kowalska, Dominika Ewa Habrowska-Górczyńska, Kinga Anna Urbanek, Tomasz Ochędalski, Agnieszka Wanda Piastowska-Ciesielska. The opposite effects of angiotensin 1-9 and angiotensin 3-7 in prostate epithelial cells. Biochemical and Biophysical Research Communications. 2019; 519 (4):868-873.

Chicago/Turabian Style

Kamila Domińska; Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kinga Anna Urbanek; Tomasz Ochędalski; Agnieszka Wanda Piastowska-Ciesielska. 2019. "The opposite effects of angiotensin 1-9 and angiotensin 3-7 in prostate epithelial cells." Biochemical and Biophysical Research Communications 519, no. 4: 868-873.

Journal article
Published: 11 May 2019 in Toxins
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Deoxynivalenol (DON), known as vomitoxin, a type B trichothecene, is produced by Fusarium. DON frequently contaminates cereal grains such as wheat, maize, oats, barley, rye, and rice. At the molecular level, it induces ribosomal stress, inflammation and apoptosis in eukaryotic cells. Our findings indicate that DON modulates the viability of prostate cancer (PCa) cells and that the response to a single high dose of DON is dependent on the androgen-sensitivity of cells. DON appears to increase reactive oxygen species (ROS) production in cells, induces DNA damage, and triggers apoptosis. The effects of DON application in PCa cells are influenced by the mitogen-activated protein kinase (MAPK) and NFΚB- HIF-1α signaling pathways. Our results indicate that p53 is a crucial factor in DON-associated apoptosis in PCa cells. Taken together, our findings show that a single exposure to high concentrations of DON (2-5 µM) modulates the progression of PCa.

ACS Style

Dominika Ewa Habrowska-Górczyńska; Karolina Kowalska; Kinga Anna Urbanek; Kamila Domińska; Agata Sakowicz; Agnieszka Wanda Piastowska-Ciesielska. Deoxynivalenol Modulates the Viability, ROS Production and Apoptosis in Prostate Cancer Cells. Toxins 2019, 11, 265 .

AMA Style

Dominika Ewa Habrowska-Górczyńska, Karolina Kowalska, Kinga Anna Urbanek, Kamila Domińska, Agata Sakowicz, Agnieszka Wanda Piastowska-Ciesielska. Deoxynivalenol Modulates the Viability, ROS Production and Apoptosis in Prostate Cancer Cells. Toxins. 2019; 11 (5):265.

Chicago/Turabian Style

Dominika Ewa Habrowska-Górczyńska; Karolina Kowalska; Kinga Anna Urbanek; Kamila Domińska; Agata Sakowicz; Agnieszka Wanda Piastowska-Ciesielska. 2019. "Deoxynivalenol Modulates the Viability, ROS Production and Apoptosis in Prostate Cancer Cells." Toxins 11, no. 5: 265.

Journal article
Published: 07 February 2019 in Ecotoxicology and Environmental Safety
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Zearalenone (ZEA) - a fungal mycotoxin is reported to both cause the oxidative stress associated with death of cells as well as induction of the proliferation of cells, depending on its concentration and the type of cells. ZEA due to its structural similarity to naturally occurring estrogens is able to bind to estrogen receptors and triggers estrogen-associated signaling pathways. The aim of this study is to evaluate whether the induction of oxidative stress in normal epithelial prostate PNT1A cells is associated with estrogenic activity of ZEA. We observed that ZEA-induced oxidative stress in PNT1A cells is associated with a decrease in the oxidative stress defense enzymes expression, cell cycle arrest in G2/M cell cycle phase as well as the decreased migration of cells. The results also suggest that the observed effect might be associated with the nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB)- hypoxia inducible factor 1 alpha (HIF-1α) signaling pathway. The usage of estrogen receptor β (ERβ) selective antagonist 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]-phenol PHTPP showed that ERβ activity is able to decrease the ZEA-induced oxidative stress, but is not enough to counteract it, indicating that ZEA-induced oxidative stress is only partially associated with estrogenic activity of ZEA.

ACS Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kinga Anna Urbanek; Kamila Domińska; Agata Sakowicz; Agnieszka Wanda Piastowska-Ciesielska. Estrogen receptor β plays a protective role in zearalenone-induced oxidative stress in normal prostate epithelial cells. Ecotoxicology and Environmental Safety 2019, 172, 504 -513.

AMA Style

Karolina Kowalska, Dominika Ewa Habrowska-Górczyńska, Kinga Anna Urbanek, Kamila Domińska, Agata Sakowicz, Agnieszka Wanda Piastowska-Ciesielska. Estrogen receptor β plays a protective role in zearalenone-induced oxidative stress in normal prostate epithelial cells. Ecotoxicology and Environmental Safety. 2019; 172 ():504-513.

Chicago/Turabian Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kinga Anna Urbanek; Kamila Domińska; Agata Sakowicz; Agnieszka Wanda Piastowska-Ciesielska. 2019. "Estrogen receptor β plays a protective role in zearalenone-induced oxidative stress in normal prostate epithelial cells." Ecotoxicology and Environmental Safety 172, no. : 504-513.

Journal article
Published: 25 October 2018 in Scientific Reports
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Angiotensin 1–7 (Ang1–7) is an endogenous bioactive component of the renin-angiotensin system (RAS). In addition to its cardiovascular properties, its anti-proliferative and anti-angiogenic traits are believed to play important roles in carcinogenesis. The present study examines the influence of Ang1–7 on processes associated with development and progression of prostate cancer cells. Our findings indicate that while Ang1–7 (1 nM; 48 h) can effectively reduce cell proliferation in DU-145, it can induce a significant decrease in the expression of MKI67 in LNCaP. In both cell lines we also observed a reduction in colony size in soft agar assay. A various changes in gene expression were noted after exposure to Ang1–7: those of anti- and pro-apoptotic agents and the NF-kB family of transcription factors, as well as mesenchymal cell markers and vascular endothelial growth factor A (VEGFA). In addition, Ang1–7 was found to modulate cell adhesion and matrix metallopeptidase (MMP) activity. Changes were also observed in the levels of angiotensin receptors and sex steroid hormone receptors. Ang1–7 reduced the levels of estrogen receptor alpha gene (ESR1) and increased the expression of estrogen receptor beta gene (ESR2) in all prostate cancer cells; it also up-regulated androgen receptor (AR) expression in androgen-sensitive cells but contradictory effect was observed in androgen- irresponsive cell lines. In summary, the results confirm the existence of complex network between the various elements of the local RAS and the molecular and cellular mechanisms of prostate cancerogenesis. The response of cancer cells to Ang1–7 appears to vary dependently on the dose and time of incubation as well as the aggressiveness and the hormonal status of cells.

ACS Style

Kamila Domińska; Piotr Okła; Karolina Kowalska; Dominika Habrowska-Górczyńska; Kinga Urbanek; Tomasz Ochędalski; Agnieszka Piastowska-Ciesielska. Angiotensin 1–7 modulates molecular and cellular processes central to the pathogenesis of prostate cancer. Scientific Reports 2018, 8, 1 -12.

AMA Style

Kamila Domińska, Piotr Okła, Karolina Kowalska, Dominika Habrowska-Górczyńska, Kinga Urbanek, Tomasz Ochędalski, Agnieszka Piastowska-Ciesielska. Angiotensin 1–7 modulates molecular and cellular processes central to the pathogenesis of prostate cancer. Scientific Reports. 2018; 8 (1):1-12.

Chicago/Turabian Style

Kamila Domińska; Piotr Okła; Karolina Kowalska; Dominika Habrowska-Górczyńska; Kinga Urbanek; Tomasz Ochędalski; Agnieszka Piastowska-Ciesielska. 2018. "Angiotensin 1–7 modulates molecular and cellular processes central to the pathogenesis of prostate cancer." Scientific Reports 8, no. 1: 1-12.

Journal article
Published: 10 October 2018 in Environmental Toxicology and Pharmacology
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A major challenge in the management of prostate cancer (PC) is to limit tumor growth and metastases. Targeted therapies applying natural compounds might be potentially useful in PC treatment. Methylsulfonylmethane (MSM), also known as organic sulfur, is a dietary supplement used for various clinical purposes, mostly known for its anti-inflammatory properties. Therefore, we decided to evaluate the effect of MSM on PC cells LNCaP, PC3 and DU-145, which represent different in vitro models of PC. We observed that MSM decreases the viability and invasiveness of PC cells through the induction of apoptosis and cell cycle arrest in the G0/G1 cell cycle phase. Moreover, MSM in a low dose (200 mM) is able to reduce the migration and invasion of PC cells. Considering the low overall body toxicity and insignificant side effects of MSM, its apoptosis-inducing properties might be used in PC treatment in the future.

ACS Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kamila Domińska; Kinga Anna Urbanek; Agnieszka Wanda Piastowska-Ciesielska. Methylsulfonylmethane (organic sulfur) induces apoptosis and decreases invasiveness of prostate cancer cells. Environmental Toxicology and Pharmacology 2018, 64, 101 -111.

AMA Style

Karolina Kowalska, Dominika Ewa Habrowska-Górczyńska, Kamila Domińska, Kinga Anna Urbanek, Agnieszka Wanda Piastowska-Ciesielska. Methylsulfonylmethane (organic sulfur) induces apoptosis and decreases invasiveness of prostate cancer cells. Environmental Toxicology and Pharmacology. 2018; 64 ():101-111.

Chicago/Turabian Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kamila Domińska; Kinga Anna Urbanek; Agnieszka Wanda Piastowska-Ciesielska. 2018. "Methylsulfonylmethane (organic sulfur) induces apoptosis and decreases invasiveness of prostate cancer cells." Environmental Toxicology and Pharmacology 64, no. : 101-111.

Research article
Published: 13 August 2018 in Journal of Cellular Biochemistry
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In mammalian cells, angiotensin II (AngII) binds to 2 distinct high‐affinity plasma membrane receptors: angiotensin receptor 1 (AT1R) and angiotensin receptor 2 (AT2R). Healthy human endometrium from women of reproductive age expresses all of the components of the renin‐angiotensin system. Many studies suggest that AngII, acting via AT1R, may have a role in the development and progression of cancer, which changes the expression of angiogenic factors, AngII and AT1R are correlated with the presence of endometrial cancer (EC). The aim of the current study was to identify the effects of AngII on the proliferation, cell cycle progression, apoptosis and mobility of ISHIKAWA, MFE296 and MFE280 EC cells with silenced AT1R. It also examines epithelial‐mesenchymal transition markers by gene expression analysis. The obtained results suggest that the silencing of AT1R expression alters the migration and invasion ability of EC cells. However, this silencing is not sufficient to inhibit the effects of AngII on EC cells, suggesting that AngII plays a more complex role in the development of EC.

ACS Style

Zuzanna E. Matysiak‐Burzyńska; Magdalena Nowakowska; Kamila Domińska; Karolina Kowalska; Elżbieta Płuciennik; Agnieszka W. Piastowska‐Ciesielska. Silencing of angiotensin receptor 1 interferes with angiotensin II oncogenic activity in endometrial cancer. Journal of Cellular Biochemistry 2018, 119, 9110 -9121.

AMA Style

Zuzanna E. Matysiak‐Burzyńska, Magdalena Nowakowska, Kamila Domińska, Karolina Kowalska, Elżbieta Płuciennik, Agnieszka W. Piastowska‐Ciesielska. Silencing of angiotensin receptor 1 interferes with angiotensin II oncogenic activity in endometrial cancer. Journal of Cellular Biochemistry. 2018; 119 (11):9110-9121.

Chicago/Turabian Style

Zuzanna E. Matysiak‐Burzyńska; Magdalena Nowakowska; Kamila Domińska; Karolina Kowalska; Elżbieta Płuciennik; Agnieszka W. Piastowska‐Ciesielska. 2018. "Silencing of angiotensin receptor 1 interferes with angiotensin II oncogenic activity in endometrial cancer." Journal of Cellular Biochemistry 119, no. 11: 9110-9121.

Journal article
Published: 01 July 2018 in Biochemical and Biophysical Research Communications
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The ACE2/Ang1-7/MAS axis was involved in the cell proliferation, migration and apoptosis of many types of reproductive tissues. The research was conducted on prostate epithelial cells, immortalized by Simian Virus 40. We examined the influence of Ang 1–7 on biological properties of PNT1A cells after 24- or 48-h treatment. The employed selective antagonists of angiotensin receptors allowed evaluation of the receptor mediating Ang1-7 action. Our data clearly indicate that Ang1-7 can decrease cell proliferation and epithelial-to-mesenchymal transition of PNT1A cells via inactivation of PI3K axis and modulation of expression of the NF-kB gene family. Furthermore, it counteracts oxidant stress and inflammation in prostate cells by inhibition of VEGF expression and MMPs activation as well as by modulating the level of ERα and ERβ. On the other hand, this heptapeptide can promote cell survival by alteration of expression of anti- and pro-apoptotic members as well as compensatory up-regulation of AR expression. Summary, the results confirm the existence of a complicated dependence networks between the various elements of the local RAS and steroid hormone receptor pathways in prostate gland. Furthermore, shows the chances of using ACE2/Ang1-7/MAS pathway as a novel therapeutic target in prevention and treatment of prostate diseases.

ACS Style

Kamila Domińska; Piotr Okła; Karolina Kowalska; Dominika Habrowska-Górczyńska; Kinga Urbanek; Tomasz Ochędalski; Agnieszka Piastowska-Ciesielska. Influence and mechanism of Angiotensin 1-7 on biological properties of normal prostate epithelial cells. Biochemical and Biophysical Research Communications 2018, 502, 152 -159.

AMA Style

Kamila Domińska, Piotr Okła, Karolina Kowalska, Dominika Habrowska-Górczyńska, Kinga Urbanek, Tomasz Ochędalski, Agnieszka Piastowska-Ciesielska. Influence and mechanism of Angiotensin 1-7 on biological properties of normal prostate epithelial cells. Biochemical and Biophysical Research Communications. 2018; 502 (1):152-159.

Chicago/Turabian Style

Kamila Domińska; Piotr Okła; Karolina Kowalska; Dominika Habrowska-Górczyńska; Kinga Urbanek; Tomasz Ochędalski; Agnieszka Piastowska-Ciesielska. 2018. "Influence and mechanism of Angiotensin 1-7 on biological properties of normal prostate epithelial cells." Biochemical and Biophysical Research Communications 502, no. 1: 152-159.

Review article
Published: 03 April 2018 in Journal of Applied Toxicology
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Deoxynivalenol (DON) is a type B trichothecene, produced by the Fusarium species. Exposure to DON might cause disruptive effects such as reduced weight gain, neuroendocrine changes and immune modulation in animals (rats, dogs, pigs). There is huge concern that similar effects can be observed in humans. DON is a potential regulator of intracellular steroidogenesis. It is also possible that DON will be involved in the regulation of miRNAs connected with steroidogenesis. This review summarizes the latest knowledge about the influence of DON on steroidogenesis and human hormonal balance.

ACS Style

Kinga Anna Urbanek; Dominika Ewa Habrowska-Górczyńska; Karolina Kowalska; Anna Stańczyk; Kamila Domińska; Agnieszka Wanda Piastowska-Ciesielska. Deoxynivalenol as potential modulator of human steroidogenesis. Journal of Applied Toxicology 2018, 38, 1450 -1459.

AMA Style

Kinga Anna Urbanek, Dominika Ewa Habrowska-Górczyńska, Karolina Kowalska, Anna Stańczyk, Kamila Domińska, Agnieszka Wanda Piastowska-Ciesielska. Deoxynivalenol as potential modulator of human steroidogenesis. Journal of Applied Toxicology. 2018; 38 (12):1450-1459.

Chicago/Turabian Style

Kinga Anna Urbanek; Dominika Ewa Habrowska-Górczyńska; Karolina Kowalska; Anna Stańczyk; Kamila Domińska; Agnieszka Wanda Piastowska-Ciesielska. 2018. "Deoxynivalenol as potential modulator of human steroidogenesis." Journal of Applied Toxicology 38, no. 12: 1450-1459.

Journal article
Published: 28 February 2018 in Toxins
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Zearalenone (ZEA), a mycotoxin produced in the genus Fusarium, binds to estrogen receptors (ER) and is therefore regarded as an endocrine disruptor. ZEA has also been found to modulate the proliferation and apoptosis of prostate cancer cells in a dose-dependent manner. This study evaluates whether the effect of a low dose of ZEA (0.1 and 0.001 nM) on the invasion and migration of prostate cancer cell line PC3 is associated with ERs expression. The invasion and migration was evaluated by modified Boyden chamber assay, scratch assay, gelatin zymography, Real Time qPCR (RTqPCR) and Western blot. The involvement of ERs was evaluated with the selective ER antagonists: estrogen receptor α (ERα) antagonist 1,3-bis (4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy) phenol]-1H-pyrazole dihydrochloride (MPP) and estrogen receptor β (ERβ) antagonist 4-[2–phenyl-5,7–bis (trifluoromethyl) pyrazolo [1,5-a]-pyrimidin-3-yl] phenol (PHTPP). ZEA was found to modulate cell motility dependent on estrogen receptors, particularly ERα. Increased cell migration and invasion were associated with increased MMP-2 and MMP-9 activity as well as the up-regulation of the EMT-associated genes vimentin (VIM), zinc finger E-box-binding homeobox 1/2 (ZEB1/2) and transforming growth factor β 1 (TGFβ1). In conclusion, ZEA might modulate the invasiveness of prostate cancer cells dependently on ERα expression.

ACS Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kinga Anna Urbanek; Kamila Domińska; Agnieszka Wanda Piastowska-Ciesielska. Estrogen Receptor α Is Crucial in Zearalenone-Induced Invasion and Migration of Prostate Cancer Cells. Toxins 2018, 10, 98 .

AMA Style

Karolina Kowalska, Dominika Ewa Habrowska-Górczyńska, Kinga Anna Urbanek, Kamila Domińska, Agnieszka Wanda Piastowska-Ciesielska. Estrogen Receptor α Is Crucial in Zearalenone-Induced Invasion and Migration of Prostate Cancer Cells. Toxins. 2018; 10 (3):98.

Chicago/Turabian Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kinga Anna Urbanek; Kamila Domińska; Agnieszka Wanda Piastowska-Ciesielska. 2018. "Estrogen Receptor α Is Crucial in Zearalenone-Induced Invasion and Migration of Prostate Cancer Cells." Toxins 10, no. 3: 98.

Journal article
Published: 25 September 2017 in International Journal of Molecular Medicine
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Angiotensin II (AngII), the main peptide of the renin‑angiotensin system (RAS), is involved in the proliferation of different types of cells, normal and pathological as well. The protein tyrosine kinases (PTKs) play an important role in the growth, differentiation and apoptosis of cells. AngII action depends on the hormonal milieu of the cell, and on sex steroid influence. Angiotensin 1‑7 (Ang1‑7), metabolite of AngII, shows opposite action to AngII in cells. The present study aimed to examine the influence of 17β‑estradiol and testosterone on AngII and Ang1‑7 action on PTK activity in androgen‑independent humane prostate cancer cell line DU145. Cell cultures of human prostate cancer DU145 cells were used as a source of PTKs. Cultures were exposed to different concentrations of AngII (5x10‑11 to 5x10‑9 M). The incubation with hormones lasted 15 min to limit the genomic effects of steroids. In the phosphorylation reaction, we used γ32P‑ATP as a donor of phosphate and a synthetic peptide, Poly(Glu, Tyr) (4:1), as a substrate. The specific activities of PTKs were defined as pmol of 32P incorporated into 1 mg of exogenous Poly(Glu, Tyr) per minute (pmol/mg/min). Our findings suggest that testosterone and 17β‑estradiol may change the effects of angiotensins in a rapid non‑genomic way, probably via membrane‑located receptors. The most significant change was caused by testosterone, whose effect was most significant on changes caused by Ang1‑7. AngII‑induced changes in phosphorylation appeared to be insensitive to the presence of testosterone, but were modified by 17β‑estradiol.

ACS Style

Kamila Domińska; Antoni Kowalski; Tomasz Ochędalski; Elżbieta Rębas. Effects of testosterone and 17β-estradiol on angiotensin-induced changes in tyrosine kinase activity in the androgen-independent human prostate cancer cell line, DU145. International Journal of Molecular Medicine 2017, 40, 1573 -1581.

AMA Style

Kamila Domińska, Antoni Kowalski, Tomasz Ochędalski, Elżbieta Rębas. Effects of testosterone and 17β-estradiol on angiotensin-induced changes in tyrosine kinase activity in the androgen-independent human prostate cancer cell line, DU145. International Journal of Molecular Medicine. 2017; 40 (5):1573-1581.

Chicago/Turabian Style

Kamila Domińska; Antoni Kowalski; Tomasz Ochędalski; Elżbieta Rębas. 2017. "Effects of testosterone and 17β-estradiol on angiotensin-induced changes in tyrosine kinase activity in the androgen-independent human prostate cancer cell line, DU145." International Journal of Molecular Medicine 40, no. 5: 1573-1581.

Journal article
Published: 01 August 2017 in Chemosphere
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Zearalenone (ZEA) is a nonsteroidal mycotoxin produced by several fungi of the genus Fusarium spp. It is known to play various roles in the regulation of the prostate cancer cell cycle, including carcinogenesis. The present study evaluates the influence of ZEA on the mitochondrial metabolism, plasma membrane permeabilization and cell cycle of prostate cancer cells. At concentrations of 100 nM and 0.3 nM, ZEA caused a decrease in the oxidative activity of mitochondria, as well as increases in LDH release, apoptosis induction and the number of cells in the G0/G1 phase. The opposite effect was observed for lower concentrations (0.1 nM and 0.001 nM). These in vitro studies indicate that ZEA might have pro- and antiproliferative properties in prostate cancer cells, at concentrations 0.1 nM, 0.001 nM and 0.3 nM, 100 nM, respectively.

ACS Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kamila Domińska; Agnieszka Wanda Piastowska-Ciesielska. The dose-dependent effect of zearalenone on mitochondrial metabolism, plasma membrane permeabilization and cell cycle in human prostate cancer cell lines. Chemosphere 2017, 180, 455 -466.

AMA Style

Karolina Kowalska, Dominika Ewa Habrowska-Górczyńska, Kamila Domińska, Agnieszka Wanda Piastowska-Ciesielska. The dose-dependent effect of zearalenone on mitochondrial metabolism, plasma membrane permeabilization and cell cycle in human prostate cancer cell lines. Chemosphere. 2017; 180 ():455-466.

Chicago/Turabian Style

Karolina Kowalska; Dominika Ewa Habrowska-Górczyńska; Kamila Domińska; Agnieszka Wanda Piastowska-Ciesielska. 2017. "The dose-dependent effect of zearalenone on mitochondrial metabolism, plasma membrane permeabilization and cell cycle in human prostate cancer cell lines." Chemosphere 180, no. : 455-466.

Journal article
Published: 11 July 2017 in Acta Biochimica Polonica
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The aim of this study was to expand our knowledge about anticancer activity of some polyamine derivatives with quinoline or chromane as terminal moieties. Tested compounds were evaluated in vitro towards metastatic human prostate adenocarcinoma (PC3), human carcinoma (DU145) and mammary gland adenocarcinoma (MCF7) cell lines. Cell viability was estimated on the basis of mitochondrial metabolic activity using watersoluble tetrazolium WST1 to establish effective concentrations of the tested compounds under experimental conditions. Cytotoxic potential of polyamine derivatives was determined by the measurement of lactate dehydrogenase activity released from damaged cells, changes in mitochondrial membrane potential, the cell cycle distribution analysis and apoptosis assay. It was revealed that the tested polyamine derivatives differed markedly in their antiproliferative activity. Bischromane derivative 5a exhibited a rather cytostatic than cytotoxic effect on the tested cells, whereas quinoline derivative 3a caused changes in cell membrane integrity, inhibited cell cycle progression, as well as induced apoptosis of prostate and breast cancer cells which suggest its potential application in cancer therapy.

ACS Style

Marta Szumilak; Małgorzata Gałdyszyńska; Kamila Dominska; Andrzej Stanczak; Agnieszka Wanda Piastowska-Ciesielska. Anticancer activity of some polyamine derivatives on human prostate and breast cancer cell lines. Acta Biochimica Polonica 2017, 64, 307 -313.

AMA Style

Marta Szumilak, Małgorzata Gałdyszyńska, Kamila Dominska, Andrzej Stanczak, Agnieszka Wanda Piastowska-Ciesielska. Anticancer activity of some polyamine derivatives on human prostate and breast cancer cell lines. Acta Biochimica Polonica. 2017; 64 (2):307-313.

Chicago/Turabian Style

Marta Szumilak; Małgorzata Gałdyszyńska; Kamila Dominska; Andrzej Stanczak; Agnieszka Wanda Piastowska-Ciesielska. 2017. "Anticancer activity of some polyamine derivatives on human prostate and breast cancer cell lines." Acta Biochimica Polonica 64, no. 2: 307-313.

Journal article
Published: 12 May 2017 in Molecules
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Polyamine conjugates with bicyclic terminal groups including quinazoline, naphthalene, quinoline, coumarine and indole have been obtained and their cytotoxic activity against PC–3, DU–145 and MCF–7 cell lines was evaluated in vitro. Their antiproliferative potential differed markedly and depended on both their chemical structure and the type of cancer cell line. Noncovalent DNA-binding properties of the most active compounds have been examined using ds–DNA thermal melting studies and topo I activity assay. The promising biological activity, DNA intercalative binding mode and favorable drug-like properties of bis(naphthalene-2-carboxamides) make them a good lead for further development of potential anticancer drugs.

ACS Style

Marta Szumilak; Małgorzata Gałdyszyńska; Kamila Dominska; Irena I. Bak-Sypien; Anna Merecz-Sadowska; Andrzej Stańczak; Boleslaw T. Karwowski; Agnieszka W. Piastowska-Ciesielska. Synthesis, Biological Activity and Preliminary in Silico ADMET Screening of Polyamine Conjugates with Bicyclic Systems. Molecules 2017, 22, 794 .

AMA Style

Marta Szumilak, Małgorzata Gałdyszyńska, Kamila Dominska, Irena I. Bak-Sypien, Anna Merecz-Sadowska, Andrzej Stańczak, Boleslaw T. Karwowski, Agnieszka W. Piastowska-Ciesielska. Synthesis, Biological Activity and Preliminary in Silico ADMET Screening of Polyamine Conjugates with Bicyclic Systems. Molecules. 2017; 22 (5):794.

Chicago/Turabian Style

Marta Szumilak; Małgorzata Gałdyszyńska; Kamila Dominska; Irena I. Bak-Sypien; Anna Merecz-Sadowska; Andrzej Stańczak; Boleslaw T. Karwowski; Agnieszka W. Piastowska-Ciesielska. 2017. "Synthesis, Biological Activity and Preliminary in Silico ADMET Screening of Polyamine Conjugates with Bicyclic Systems." Molecules 22, no. 5: 794.

Journal article
Published: 26 April 2017 in Molecular Medicine Reports
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An increasing number of researchers are focusing on the influence of local peptide hormones such as angiotensin II (Ang II) and relaxin 2 (RLN2) in the regulation of inflammation and carcinogenesis. The interaction between the renin‑angiotensin system (RAS) and relaxin family peptide system (RFPS) is known to influence the proliferation, adhesion and migration of normal and cancer prostate cell lines. The aim of the present study was to evaluate changes in the expression of nuclear factor‑κB subunit 1 (NFKB1), nuclear factor‑κB subunit 2 (NFKB2), REL proto‑oncogene nuclear factor‑κB p65 subunit (REL), RELA proto‑oncogene nuclear factor‑κB subunit (RELA) and RELB proto‑oncogene nuclear factor‑κB subunit (RELB) mRNA caused by Ang II and RLN2. The members of NF‑kB family are involved in many processes associated with cancer development and metastasis. Reverse transcription‑quantitative polymerase chain reaction analysis identified that both peptide hormones have an influence on the relative expression of nuclear factor‑κB. Following treatment with either peptide, NFKB1 expression was downregulated in all prostate cancer cell lines (LNCaP, DU‑145 and PC3), but not in normal epithelial cells (PNT1A). Conversely, RELB mRNA was enhanced only in non‑cancerous prostate cells. RELA expression was strongly stimulated in the most aggressive cell line, whereas REL mRNA was unchanged. In many cases, the effect was strictly dependent on the cell line and/or the type of peptide: Ang II increased expression of both RELA and REL genes in the androgen‑dependent cell line while RLN2 enhanced NFKB2 and RELA mRNA in androgen‑independent cells (DU‑145). Further research is needed to understand the regulation of NF‑κB family members by key renin‑angiotensin system and RFPS peptides in prostate cancer cells; however, prostate carcinogenesis appears to be influenced by the balance between the cross‑regulation of nuclear factor‑κB (NF‑κB) and androgen receptor pathways by Ang II and relaxin 2.

ACS Style

Kamila Domińska; Karolina Kowalska; Zuzanna Elżbieta Matysiak; Elżbieta Płuciennik; Tomasz Ochędalski; Agnieszka Piastowska-Ciesielska. Regulation of mRNA gene expression of members of the NF-κB transcription factor gene family by angiotensin II and relaxin 2 in normal and cancer prostate cell lines. Molecular Medicine Reports 2017, 15, 4352 -4359.

AMA Style

Kamila Domińska, Karolina Kowalska, Zuzanna Elżbieta Matysiak, Elżbieta Płuciennik, Tomasz Ochędalski, Agnieszka Piastowska-Ciesielska. Regulation of mRNA gene expression of members of the NF-κB transcription factor gene family by angiotensin II and relaxin 2 in normal and cancer prostate cell lines. Molecular Medicine Reports. 2017; 15 (6):4352-4359.

Chicago/Turabian Style

Kamila Domińska; Karolina Kowalska; Zuzanna Elżbieta Matysiak; Elżbieta Płuciennik; Tomasz Ochędalski; Agnieszka Piastowska-Ciesielska. 2017. "Regulation of mRNA gene expression of members of the NF-κB transcription factor gene family by angiotensin II and relaxin 2 in normal and cancer prostate cell lines." Molecular Medicine Reports 15, no. 6: 4352-4359.

Journal article
Published: 21 June 2016 in Oncology Reports
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Spandidos Publications is a scientific publisher with a long-standing international reputation for excellent standards and high quality science publications

ACS Style

Magdalena Nowakowska; Zuzanna Matysiak-Burzyńska; Karolina Kowalska; Elżbieta Płuciennik; Kamila Domińska; Agnieszka Piastowska-Ciesielska. Angiotensin II promotes endometrial cancer cell survival. Oncology Reports 2016, 36, 1101 -1110.

AMA Style

Magdalena Nowakowska, Zuzanna Matysiak-Burzyńska, Karolina Kowalska, Elżbieta Płuciennik, Kamila Domińska, Agnieszka Piastowska-Ciesielska. Angiotensin II promotes endometrial cancer cell survival. Oncology Reports. 2016; 36 (2):1101-1110.

Chicago/Turabian Style

Magdalena Nowakowska; Zuzanna Matysiak-Burzyńska; Karolina Kowalska; Elżbieta Płuciennik; Kamila Domińska; Agnieszka Piastowska-Ciesielska. 2016. "Angiotensin II promotes endometrial cancer cell survival." Oncology Reports 36, no. 2: 1101-1110.